The use of biodegradable polyurethane scaffolds for cartilage tissue engineering: potential and limitations

Grad, Sibylle; Kupcsik, László; Górna, Katarzyna; Gogolewski, Sylwester; Alini, Mauro

doi:10.1016/s0142-9612(03)00462-9

Cited by 264 publications

(160 citation statements)

References 56 publications

Supporting

Mentioning

155

Contrasting

Unclassified

Order By: Relevance

“…However, it is believed that synthesized proteins diffuse out of the highly porous structure and cannot be sufficiently incorporated into the extracellular matrix, resulting in a prolonged limiting effect of the material (Grad et al, 2003). Here, spheroids were combined with fibrin hydrogel and already showed in the first week of culture a migration from PU particles into the fibrin matrix.…”

Section: Discussionmentioning

confidence: 99%

Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Mauth

Bono²,

Haas³

et al. 2009

eCM

View full text Add to dashboard Cite

Nowadays, intervertebral disc (IVD) degeneration is one of the principal causes of low back pain involving high expense within the health care system. The long-term goal is the development of a medical treatment modality focused on a more biological regeneration of the inner nucleus pulposus (NP). Hence, interest in the endoscopic implantation of an injectable material took center stage in the recent past. We report on the development of a novel polyurethane (PU) scaffold as a mechanically stable carrier system for the reimplantation of expanded autologous IVDderived cells (disc cells) to stimulate regenerative processes and restore the chondrocyte-like tissue within the NP. Primary human disc cells were seeded into newly developed PU spheroids which were subsequently encapsulated in fibrin hydrogel. The study aims to analyze adhesion properties, proliferation capacity and phenotypic characterization of these cells. Polymerase chain reaction was carried out to detect the expression of genes specifically expressed by native IVD cells. Biochemical analyses showed an increased DNA content, and a progressive enhancement of total collagen and glycosaminoglycans (GAG) was observed during cell culture. The results suggest the synthesis of an appropriate extracellular matrix as well as a stable mRNA expression of chondrogenic and/or NP specific markers. In conclusion, the data presented indicate an alternative medical approach to current treatment options of degenerated IVD tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Mauth

Bono²,

Haas³

et al. 2009

eCM

View full text Add to dashboard Cite

show abstract

“…Another primary materials studied mostly to fabricate scaffolds are polymers such as polylactic acid [35,36], polyglycolic acid [37], polyurethane [38], and a number of copolymers [39][40][41]. Natural polymer-based scaffolds have excellent bioactivity, biodegradability but poor mechanical properties.…”

Section: Bioceramic Scaffold Materialsmentioning

confidence: 99%

Bioceramic Scaffolds

Amin¹,

Ewais²

2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

View full text Add to dashboard Cite

Millions of peoples in the world suffer from their bone damage tissues by disease or trauma. Every day, thousands of surgical procedures are performed to replace or repair these tissues. The availability of these tissues is a big problem, and their costs are expensive. The repair of these defects has become a major clinical and socioeconomic need with the increase of aging population and social development. The emerge of tissue engineering (TE) is considered as a glimmer of hope to contribute in solving this problem. It aims at the regeneration of damaged tissues with restoring and maintaining the function of human bone tissues using the combination of cell biology, materials science, and engineering principles. In this chapter, the current state of the tissue engineering in particular bioceramic scaffolds was discussed. Concept of tissue engineering was explored. Bioceramic scaffold materials, their processing techniques, challenges taken into consideration the design of the scaffolds, and their in-vitro and in-vivo studies were highlighted. The scaffolds with extra-functionalities such as drug release ability and clinical applications were mentioned.

show abstract

“…The amount of hard segments influences the degree of phase separation, which in turn affects physical and mechanical properties [30], degradation rate and biocompatibility [32][33]. By varying the molecular weight of polyol and the composition of the different segments, properties of PUR can be tuned up for use in many areas of TE [34][35][36][37][38]. In this respect, the range of mechanical and morphological properties that can be obtained with PUR is significantly larger than with commonly used medical grade biodegradable polymers for example: PLA, PGA, poly(DL-lactide) (PDLLA) [39][40][41][42].…”

Section: Polyurethanes In Tementioning

confidence: 99%

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

Kucińska-Lipka¹,

St.²,

Janik³

et al. 2016

ChChT

View full text Add to dashboard Cite

Abstract. The introduction of the paper was devoted to the main items of Tissue Engineering (TE) and the way of porous structure obtaining as scaffolds. Furthermore, the significant role of the scaffold design in TE was described. It was shown, that properly designed polyurethanes (PURs) find application in TE due to the proper physicochemical, mechanical and biological properties. Then the use of L-ascorbic acid (L-AA) in PUR systems for TE was described. L-AA has been applied in this area due to its suitable biological characteristics and antioxidative properties. Moreover, L-AA influences tissue regeneration due to improving collagen synthesis, which is a primary component of the extracellular matrix (ECM). Modification of PUR with L-AA leads to the materials with higher biocompatibility and such system is promising for TE applications.

show abstract

The use of biodegradable polyurethane scaffolds for cartilage tissue engineering: potential and limitations

Cited by 264 publications

References 56 publications

Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Cell-seeded polyurethane-fibrin structures – A possible system for intervertebral disc regeneration

Bioceramic Scaffolds

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

Contact Info

Product

Resources

About