The use of bull spermatozoa suspension for cytotoxicity evaluation

Es'kov, A. P.; Kayumov, Rinat; Sokolov, Anatoly

doi:10.1016/j.toxlet.2008.06.414

Cited by 3 publications

(2 citation statements)

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“…Mouse spermatozoa could be explored as it has been recently demonstrated that they can detect bisphenol A toxicity , but that would require castration or killing of animals to retrieve the spermatozoa from the epididymis . Bull spermatozoa have also been reported as detectors of toxic compounds but with a proven lower sensitivity to detect cytotoxicity compared to boar spermatozoa . Both pig and cattle breeding are based on AI, but while AI in porcine is performed with liquid‐refrigerated semen , frozen‐thawed semen is mostly used in cattle .…”

Section: Why Using Boar Spermatozoa In Drug‐induced Toxicity Assessment?mentioning

confidence: 99%

The Usefulness of Sperm Kinematics in Drug‐Induced Toxicity Assessment

Vicente-Carrillo¹

2018

Basic Clin Pharma Tox

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In vitro drug-induced toxicity assessment demands the usability and validation of suitable cell models, obtained non-invasively and pain-free. Sperm suspensions, painlessly and easily obtained from breeding boars, have been proven as suitable biosensors for pre-clinical toxicology screening and ranking of lead compounds in the drug development processes on a kinematics-based assay. Having a limited number of mitochondria and depending on these mitochondria and on cytoplasmic glycolysis for the energy needed for motility and for plasma membrane functionality, spermatozoa become a suitable model for capturing multiple modes of action of drugs and other chemicals acting via measurements of sperm motility. In this MiniReview, the usability of boar spermatozoa as detectors of cytotoxicity based on sperm motility measurements is discussed.

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Section: Why Using Boar Spermatozoa In Drug‐induced Toxicity Assessment?mentioning

confidence: 99%

The Usefulness of Sperm Kinematics in Drug‐Induced Toxicity Assessment

Vicente-Carrillo¹

2018

Basic Clin Pharma Tox

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“…Mouse spermatozoa, recently demonstrated to detect bisphenol A toxicity by a CatSper dependent mechanism (Wang et al 2016), might be used as detectors of toxicity, but requires castration or killing of animals to retrieve the spermatozoa from the epididymis (Dusells and Vrana 2007). Bull spermatozoa have also been reported as detectors of toxic compounds (Eskov et al 2008(Eskov et al , 2009), but with a proven lower sensitivity to detect toxic compounds compared to boar spermatozoa (Jääskeläinen et al 2003). Both pig and cattle breeding rely on AI, but while AI in pigs is performed with liquid semen (Wagner and Thibier 2000), frozen/thawed semen is mostly used in cattle (Rodríguez-Martínez and Barth 2007).…”

Section: Discussionmentioning

confidence: 99%

Sperm Membrane Channels, Receptors and Kinematics : Using boar spermatozoa for drug toxicity screening

Vicente-Carrillo¹

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About the cover:The figure in the front cover displays boar spermatozoa where AQP-9 (upper-left corner), CatSper 3 (upper-right corner) and the δ-opioid receptor (lower-left corner) are immunolocalized. A representative output of the Qualisperm™ software depicting each detected spermatozoon and its motility trajectory (in yellow) is provided in the lower-right corner.During the course of the research underlying this Thesis, Alejandro Vicente Carrillo was enrolled in Forum Scientium, a multidisciplinary doctoral program at Linköping University. Printed by LiU-Tryck, Linköping, Sweden, 2016 To my family MAIN SUPERVISOR Heriberto Rodríguez-Martínez ABSTRACTInternal fertilization usually implies that a spermatozoon, with intact attributes for zygote formation, passes all hurdles during its transport through the female genitalia and reaches the oocyte. During this journey, millions to billions of other spermatozoa perish. Spermatozoa are highly differentiated motile cells without synthetic capabilities. They generate energy via glycolysis and oxidative phosphorylation to sustain motility and to maintain the stability and functionality of their plasma membrane. In vivo, they spend their short lifespan bathing in female genital tract fluids of different origins, or are in vitro exposed to defined media during diverse sperm handling i.e. extension, cryopreservation, in vitro fertilization, etc. Being excitable cells, spermatozoa respond in vivo to various stimuli during pre-fertilization (capacitation, hyperactivation, oocyte location) and fertilization (acrosome reaction, interaction with the oocyte) events, mediated via diverse membrane ion-conducting channels and ligand-gated receptors. The present Thesis has mapped the presence and reactivity (sperm intactness and kinematics) of selected receptors, water and ion channels in ejaculated boar spermatozoa. The final aim was to find a relevant alternative cell type for in vitro bioassays that could ease the early scrutiny of candidate drugs as well as decreasing our needs for experimental animals according to the 3R principles. Spermatozoa are often extended, cooled and thawed to warrant their availability as fertile gametes for breeding or in vitro testing. Such manipulations stress the cells via osmotic variations and hence spermatozoa need to maintain membrane intactness by controlling the exchange of water and the common cryoprotectant glycerol, via aquaporins (AQPs). Both AQPs-7 and -9 were studied for membrane domain changes in cauda-and ejaculated spermatozoa (un-processed, extended, chilled or frozen-thawed). While AQP-9 maintained location through source and handling, thawing of ejaculated spermatozoa clearly relocated the labelling of AQP-7, thus appearing as a relevant marker for non-empirical studies of sperm cryopreservation. Alongside water, spermatozoa interact with calcium (Ca 2+ ) via the main Ca 2+ sperm channel CatSper. Increments in intracellular Ca 2+ initiate motility hyperactivation and the acrosome reaction. The four subunits of the CatSper ch...

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