The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids

Plante, Marc; Bailey, Bruce; Acworth, Ian N.

doi:10.1007/978-1-60761-322-0_23

Cited by 21 publications

(16 citation statements)

References 14 publications

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“…A higher content of phospholipids was found in the yolk of examined eggs compared to those of Adeyeye (250 mg 100 g –1 or 2.5 g kg −1 ), El Bagir et al . (97.03 mg g –1 ) and Plante et al . (86.6 mg g –1 ).…”

Section: Resultsmentioning

confidence: 79%

Comparative analysis of nutrient content and energy of eggs from different chicken genotypes

et al. 2019

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BACKGROUND Eggs are important foods in the daily diet of humans and have great biological activity and a high digestibility. Egg yolk is a good source of biologically active substances such as fatty acids, phospholipids, sterols and tocopherols. The eggs of seven chicken genotypes were analyzed for their chemical composition, and a detailed study of the lipids in egg yolk was conducted. RESULTS Energy composition of the egg yolk and egg albumen was 29.06–30.51 MJ kg−1 and 19.77–20.93 MJ kg−1 respectively. Regarding their chemical composition: water ranged from 471.7 to 515.4 g kg−1 and 878.3–885.9 g kg−1; fat content in dry matter ranged from 607 to 647 g kg−1 and 6.7–11.6 g kg−1; protein varied from 302 to 331.7 g kg−1 and 823.6–892.5 g kg−1; ash ranged from 33.7 to 37.7 g kg−1 and 63.8–74.0 g kg−1; and nitrogen‐free extracts ranged from 12.7 to 36.5 g kg−1 and 35.0–96.2 g kg−1. The sterols and phospholipids in the yolk lipids were 16–26 g kg−1 and 59–127 g kg−1. The main fatty acids in the lipids were oleic (39.1–47.3%) and palmitic (26.0–35.5%) acids. Cholesterol in the yolk lipids ranged from 15.9 to 25.9 g kg−1. Phosphatidylcholine (389–573 g kg−1), phosphatidylethanolamine (219–355 g kg−1) and phosphatidylinositol (112–284 g kg−1) were the main phospholipids. The content of saturated fatty acids in the phospholipids was significantly higher than that in triacylglycerols. CONCLUSION Small variations in the chemical composition of eggs from seven different genotypes were observed. Significant differences in the fatty acid compositions of the main classes of phospholipids and the triacylglycerol fraction were established. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 79%

Comparative analysis of nutrient content and energy of eggs from different chicken genotypes

et al. 2019

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“…The lipid-containing chloroform fraction was analyzed by normal-phase HPLC. Phosphatidylcholine and phosphatidylethanolamine levels in the samples were calculated using an Ultimate 3000 high performance liquid chromatography system (Thermo, Germering, Germany) coupled with a Corona charged aerosol detector instrument (Thermo, Chelmsford, MA, USA) [46]. Chromatographic separation was carried out using a Phenomenox Silica (150 x 4.6 mm; 3μ) column at a flow rate of 1 ml/min.…”

Section: Methodsmentioning

confidence: 99%

Iron-Regulated Phospholipase C Activity Contributes to the Cytolytic Activity and Virulence of Acinetobacter baumannii

et al. 2016

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Acinetobacter baumannii is an opportunistic Gram-negative pathogen that causes a wide range of infections including pneumonia, septicemia, necrotizing fasciitis and severe wound and urinary tract infections. Analysis of A. baumannii representative strains grown in Chelex 100-treated medium for hemolytic activity demonstrated that this pathogen is increasingly hemolytic to sheep, human and horse erythrocytes, which interestingly contain increasing amounts of phosphatidylcholine in their membranes. Bioinformatic, genetic and functional analyses of 19 A. baumannii isolates showed that the genomes of each strain contained two phosphatidylcholine-specific phospholipase C (PC-PLC) genes, which were named plc1 and plc2. Accordingly, all of these strains were significantly hemolytic to horse erythrocytes and their culture supernatants tested positive for PC-PLC activity. Further analyses showed that the transcriptional expression of plc1 and plc2 and the production of phospholipase and thus hemolytic activity increased when bacteria were cultured under iron-chelation as compared to iron-rich conditions. Testing of the A. baumannii ATCC 19606T plc1::aph-FRT and plc2::aph isogenic insertion derivatives showed that these mutants had a significantly reduced PC-PLC activity as compared to the parental strain, while testing of plc1::ermAM/plc2::aph demonstrated that this double PC-PLC isogenic mutant expressed significantly reduced cytolytic and hemolytic activity. Interestingly, only plc1 was shown to contribute significantly to A. baumannii virulence using the Galleria mellonella infection model. Taken together, our data demonstrate that both PLC1 and PLC2, which have diverged from a common ancestor, play a concerted role in hemolytic and cytolytic activities; although PLC1 seems to play a more critical role in the virulence of A. baumannii when tested in an invertebrate model. These activities would provide access to intracellular iron stores this pathogen could use during growth in the infected host.

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“…The method was adopted from dionex.com [70] with modifications. Samples were prepared by diluting 1 mg of analyte in 1 mL of methanol/chloroform (1:1, v / v ).…”

Section: Methodsmentioning

confidence: 99%

An Extract from Shrimp Processing By-Products Protects SH-SY5Y Cells from Neurotoxicity Induced by Aβ25–35

et al. 2017

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Increased evidence suggests that marine unsaturated fatty acids (FAs) can protect neurons from amyloid-β (Aβ)-induced neurodegeneration. Nuclear magnetic resonance (NMR), high performance liquid chromatography (HPLC) and gas chromatography (GC) assays showed that the acetone extract 4-2A obtained from shrimp Pandalus borealis industry processing wastes contained 67.19% monounsaturated FAs and 16.84% polyunsaturated FAs. The present study evaluated the anti-oxidative and anti-inflammatory effects of 4-2A in Aβ25–35-insulted differentiated SH-SY5Y cells. Cell viability and cytotoxicity were measured by using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Quantitative PCR and Western blotting were used to study the expression of neurotrophins, pro-inflammatory cytokines and apoptosis-related genes. Administration of 20 μM Aβ25–35 significantly reduced SH-SY5Y cell viability, the expression of nerve growth factor (NGF) and its tyrosine kinase TrkA receptor, as well as the level of glutathione, while increased reactive oxygen species (ROS), nitric oxide, tumor necrosis factor (TNF)-α, brain derived neurotrophic factor (BDNF) and its TrkB receptor. Aβ25–35 also increased the Bax/Bcl-2 ratio and Caspase-3 expression. Treatment with 4-2A significantly attenuated the Aβ25–35-induced changes in cell viability, ROS, GSH, NGF, TrkA, TNF-α, the Bax/Bcl-2 ratio and Caspase-3, except for nitric oxide, BDNF and TrKB. In conclusion, 4-2A effectively protected SH-SY5Y cells against Aβ-induced neuronal apoptosis/death by suppressing inflammation and oxidative stress and up-regulating NGF and TrKA expression.

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The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids

Cited by 21 publications

References 14 publications

Comparative analysis of nutrient content and energy of eggs from different chicken genotypes

Comparative analysis of nutrient content and energy of eggs from different chicken genotypes

Iron-Regulated Phospholipase C Activity Contributes to the Cytolytic Activity and Virulence of Acinetobacter baumannii

An Extract from Shrimp Processing By-Products Protects SH-SY5Y Cells from Neurotoxicity Induced by Aβ25–35

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