The Use of Ciprofloxacin for Decontamination of LSCC-H 32 Cell Line of Chicken Embryonal Fibroblasts Contaminated by Mycoplasma arginini

Fischer, O.; Hájková, Marie; Hořínová, Z.; Jurmanová, K; Zendulková, D.

doi:10.2754/avb199261010051

Cited by 2 publications

(5 citation statements)

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“…ся к группе катионных поверхностно-активных веществ, а именно к четвертичным аммониевым со единениям. По параметрам токсикометрии мирамистин классифицируется как малоопасное вещество 1 . Препарат активен против большинства вирусов, бактерий, грибов и простейших.…”

Section: оригинальные статьи | ящур Original Articles | Foot-and-moutunclassified

“…Исследование биологической активности антибиотиков предполагает оценку их цитотоксичности, которая проявляется в возникновении патологических изменений в клетках [1]. В зависимости от концентрации действующего вещества и восприимчивости клеточных рецепторов возможен широкий спектр изменений на уровне клетки, которые могут проявляться в виде цитостатического эффекта либо в форме цитоцидного влияния, вызывающего ее гибель [4].…”

Section: оригинальные статьи | ящур Original Articles | Foot-and-moutunclassified

“…В зависимости от концентрации действующего вещества и восприимчивости клеточных рецепторов возможен широкий спектр изменений на уровне клетки, которые могут проявляться в виде цитостатического эффекта либо в форме цитоцидного влияния, вызывающего ее гибель [4]. 1 ГОСТ 12.1.007-76. Система стандартов безопасности труда (ССБТ).…”

Section: оригинальные статьи | ящур Original Articles | Foot-and-moutunclassified

“…Данные прокариотические микроорганизмы плотно прилегают к клеточной мембране, получают из нее различные питательные вещества и ростовые факторы. Метаболиты микоплазм, в том числе перекись водорода и аммиак, накапливаются в культуральной среде и могут оказывать выраженное цитопатическое действие [1,2,3]. Микоплазмы вызывают существенные метаболические нарушения в клетках, следствием этого могут быть изменения уровня синтеза РНК и белков, состава клеточной мембраны, появление хромосомных аберраций, индукция или активация экспрессии цитокинов, резкое снижение пролиферативной активности [4].…”

Section: Introductionunclassified

“…Микоплазмы устойчивы к сульфаниламидам, пенициллинам, стрептомицину, но чувствительны к антибиотикам тетрациклинового ряда, макролидам и фторхинолонам [1,3]. В настоящее время в ветеринарной практике применяется препарат мирамистин (бензилдиметил [3-(миристоиламино)пропил] аммоний хлорид моногидрат), обладающий широким бактерицидным действием.…”

Section: Introductionunclassified

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Miramistin effect on BHK-21 and PSGK-30 cell line proliferation and FMD virus reproduction in these cells

Доронин

Лозовой

Пронин

et al. 2020

Veterinariâ segodnâ

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At present, Miramistin drug (benzyldimethyl [3-(myristoylamino) propyl] ammonium chloride monohydrate), which has a broad bactericidal effect, is used in veterinary practice. This antiseptic is active against most viruses, mycoplasmas, bacteria, fungi and protozoa. According to toxicometric parameters, Miramistin is classified as a low-hazard substance. Use of cell structures as test systems for assessing the toxicity of pharmacological substances instead of conventional tests on experimental animals allows us to better clarify the possible mechanism of the effect of the investigated substance. Since many types of mycoplasmas affect the genitourinary system organs, to assess the cytotoxicity of Miramistin kidney mammalian cells of various mammals can be used as test systems, in particular, the newborn Syrian hamster (VNK-21), Siberian mountain ibex (PSGK-30), and others. The possibility of using BHK-21 and PSGK-30 cell monolayer as test systems for assessing the baseline cytotoxicity of Miramistin was shown. When studying toxicity of the drug, the effect of its various concentrations on the cell morphology was studied, the number of viable cells and the total protein content were determined as an indicator of the cell mass increase. The results of a cytomorphological study indicate that the Miramistin maximum permissible concentration of for BHK-21 and PSGK-30 cell monolayer is 25 μg/cm3. The use of this antibacterial drug in higher concentrations caused the appearance and increased signs of endogenous intoxication and degeneration. When evaluating the proliferative activity of cells under the influence of the Miramistin antibiotic in different concentrations, it was found that increasing the dose to 50, 75, 100, 125, 150 μg/cm3 leads to decrease in the rate of cell growth compared to the control. The Miramistin content in the growth medium in an amount of up to 25 μg/cm3 did not affect the intensity of protein synthesis. Presence of Miramistin in the culture medium at the maximum permissible concentration causes a slight decrease in the reproduction of foot and mouth disease virus in BHK-21 and PSGK-30 cell cultures by 4.5 and 4.0%, respectively, compared with the control without antibiotic. Since mycoplasmas are the most common contaminants of cell cultures, further studies will be aimed at exploring the possibility of using Miramistin for decontamination of BHK-21 and PSGK-30 cell monolayers.

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Section: оригинальные статьи | ящур Original Articles | Foot-and-moutunclassified

Section: Introductionunclassified

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Miramistin effect on BHK-21 and PSGK-30 cell line proliferation and FMD virus reproduction in these cells

Доронин

Лозовой

Пронин

et al. 2020

Veterinariâ segodnâ

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Establishment and Characteristics of a Cell Line from Fetal Bovine Thyroid (FBTY)

Fischer¹,

Machatkova²,

Pecůchová³

et al. 1994

Acta Vet. Brno

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Fischer 0., M. Machatkova, L. Pecuchova, D. Zendulkova and Z. H 0 fin 0 va: Establishment and Characteristics ofa Cell linefrom FetalBovine Thyroid (FBIT). Acta vet. Bmo, 63, 1994: 81-87.Characteristics of a cell line from fetal bovine thyroid were tested in the 150th passage. The line was adapted to growth in the minimal essential medium supplemented with 10 % bovine fetal semm and it had an epithelioid morphology. After seeding of lxlO" cellslml, the culture density was higher than lxl()f> cellslrnl on day 6. The FBTY line was heteroploid. The average number of chromosomes was 48.8 ± 10.0 and only 1 % of mitoses had a diploid number of chromosomes (2n = 60). Species specificity (Bos taurus) was confirmed by indirect immunofluorescence. Besides pronounced fluorescence with rabbit antisera against bovine proteins, weak reactions with antisera against ovine, caprine and swine proteins were observed. The FBTY line was susceptible to two strains of parainfluenza 3 virus, six strains of infectious bovine rhinotracheitis virus and bovine adenoviruses of the serovars I, 2, 3, and 8. • line ECTC derived from fetal bovine thyroid gland which was, in passage 150, susceptible to all known serovars of bovine adenoviruses, including members of the subgroup II which are difficult to replicate. Mitosis, indirect immunofluorescence, bovine adenovirusIn 1981, Marie Machatkova in the Veterinary Research Institute, Bmo, established a cell line from fetal bovine thyroid gland and the results are described in this paper.The characteristics of this cell line (FBTY) were tested in passage 150.Replications of bovine adenoviruses in FBTY and some other bovine cell cultures were compared by P e c uchova (1993). Materials and Methods Establishment and cultureThyroid gland of a nine-month-old male bovine fetus was collected under sterile conditions immediately after exsanguination (Machatkova and PospBil 1975) and transported to the laboratory in minimal essential medium (MEM SEVAC, Institute of Sera and Vaccines, Praha) supplemented with 200 I. U. of penicillin and 200 \lg of streptomycin per m!. Tissue fragments were washed in the medium and cultured in 60 mm plastic Petri dishes in 5 ml of the EPL SEVAC medium (Institute of Sera and Vaccines, Praha) containing 10 % bovine fetal serum, 100 I. U. of penicillin and 100 \lg of streptomycin per m!. The cells were cultured at 37°C in an atmosphere containing 5 % CO 2 , The EPL was replaced after 72h by a 1: 1 mixture of EPL+MEM subsequently changed every other day.As soon as the attached fragments were surrounded by growing cells, the cells were released by 0.02 % versene with 0.25 % trypsin Difco (1:250), reseeded into Mueller's flasks and cultured further in MEM with 10 % bovine fetal serum, 100 I. U. of penicillin and 100 \lg of streptomycin per ml in 300 ml and 500 rnl Roux flasks, Legroux flasks and Mueller's flasks.In passage 85, the cells were frozen and kept for 8 years (1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988)(1989) in liquid nitrogen. When reseeded after passage 85,...

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The Use of Ciprofloxacin for Decontamination of LSCC-H 32 Cell Line of Chicken Embryonal Fibroblasts Contaminated by Mycoplasma arginini

Cited by 2 publications

References 16 publications

Miramistin effect on BHK-21 and PSGK-30 cell line proliferation and FMD virus reproduction in these cells

Miramistin effect on BHK-21 and PSGK-30 cell line proliferation and FMD virus reproduction in these cells

Establishment and Characteristics of a Cell Line from Fetal Bovine Thyroid (FBTY)

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