The use of combination therapy for the improvement of colistin activity against bacterial biofilm

Jalil, Abduladheem Turki; Alrawe, Rawaa Turki Abdulghafoor; Al-Saffar, Montaha A.; Shaghnab, Murtadha Lafta; Merza, Muna S.; Abosaooda, Munther; Latef, Rahim

doi:10.1007/s42770-023-01189-7

Cited by 4 publications

(1 citation statement)

References 134 publications

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“… 6 Additionally, monotherapy could induce resistance through the increased formation of biofilms, such as colistin. 7 Thus, PA biofilm infections are difficult to treat.…”

Section: Introductionmentioning

confidence: 99%

The Potentiation Activity of Azithromycin in Combination with Colistin or Levofloxacin Against Pseudomonas aeruginosa Biofilm Infection

Wang,

Li,

Zhang

et al. 2024

IDR

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Objective Pseudomonas aeruginosa (PA) often displays drug resistance and biofilm-mediated adaptability. Here, we aimed to evaluate the antibiofilm efficacy of azithromycin-based combination regimens. Methods Minimum inhibitory concentrations (MICs), minimal biofilm eradication concentrations (MBECs), and MBEC-combination of azithromycin, colistin, amikacin, and levofloxacin to bioluminescent strain PAO1 and carbapenem-resistant PAO1 (CRPAO1) were assessed. An animal biofilm infection model was established and detected using a live animal bio-photonic imaging system. Results In vitro, PAO1 and CRPAO1 were susceptible to colistin, amikacin, and levofloxacin, while they were unsusceptible to azithromycin. The combinations based on azithromycin have no synergistic effect on biofilm in vitro. In vivo, azithromycin plus colistin or levofloxacin could shorten the PAO1 biofilm eradication time, which totally eradicates the biofilm in all mice on the 8 th or 6 th day, while monotherapy only eradicate biofilm in 70% or 80% mice on the 8 th day. For CRPAO1 biofilm, only azithromycin–colistin combination and colistin monotherapy eradicated the bacteria in 60% and 40% of mice at the 6 th day. Conclusion Azithromycin-based combinations containing levofloxacin or colistin had no synergistic effect in vitro, and they are promising for clinical applications due to the good synergistic activity against PAO1 biofilms in vivo.

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“… 6 Additionally, monotherapy could induce resistance through the increased formation of biofilms, such as colistin. 7 Thus, PA biofilm infections are difficult to treat.…”

Section: Introductionmentioning

confidence: 99%