The use of combinatorial topographical libraries for the screening of enhanced osteogenic expression and mineralization

Lovmand, Jette; Justesen, Jeannette; Foss, Morten; Lauridsen, Rune Hoff; Lovmand, Michael; Modin, Charlotte; Besenbacher, Flemming; Pedersen, Finn Skou; Duch, Mogens

doi:10.1016/j.biomaterials.2008.12.081

Cited by 117 publications

(97 citation statements)

References 27 publications

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“…The current TopoChip contains 2,176 unique surface topographies, which we were able to screen and analyze simultaneously. Typically, research on the effect of surface topography on cell behavior is characterized by low throughput, with some notable exceptions (24,25). Although exact calculations are difficult to make, we think that more unique surface patterns were screened for an effect on proliferation and osteogenic differentiation of hMSCs in this manuscript alone than ever published in literature.…”

Section: Discussionmentioning

confidence: 98%

An algorithm-based topographical biomaterials library to instruct cell fate

Unadkat

Hulsman

Cornelissen

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

353

374

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It is increasingly recognized that material surface topography is able to evoke specific cellular responses, endowing materials with instructive properties that were formerly reserved for growth factors. This opens the window to improve upon, in a cost-effective manner, biological performance of any surface used in the human body. Unfortunately, the interplay between surface topographies and cell behavior is complex and still incompletely understood. Rational approaches to search for bioactive surfaces will therefore omit previously unperceived interactions. Hence, in the present study, we use mathematical algorithms to design nonbiased, random surface features and produce chips of poly(lactic acid) with 2,176 different topographies. With human mesenchymal stromal cells (hMSCs) grown on the chips and using high-content imaging, we reveal unique, formerly unknown, surface topographies that are able to induce MSC proliferation or osteogenic differentiation. Moreover, we correlate parameters of the mathematical algorithms to cellular responses, which yield novel design criteria for these particular parameters. In conclusion, we demonstrate that randomized libraries of surface topographies can be broadly applied to unravel the interplay between cells and surface topography and to find improved material surfaces.

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Section: Discussionmentioning

confidence: 98%

An algorithm-based topographical biomaterials library to instruct cell fate

Unadkat

Hulsman

Cornelissen

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

353

374

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“…132,152,153 A higher survival rate of human ES cells and human induced pluripotent stem (iPS) cells in dissociated culture is the result of inhibition of ROCK-dependent 154,155 Furthermore, the degree of actin cytoskeletal tension mediated by ROCK plays a major role not only in directing stem cell differentiation to a specific cell type but also in retention of multipotency by stem cells (Table 1). 152 Some micro/nanotopographical features can increase cell proliferation concomitantly with the enhancement of osteogenic differentiation, 152,153 and others have decoupled effects, 153 although the detailed mechanism remains to be clarified. Further studies of pathways and molecules involved in actin cytoskeleton-mediated control of stem cell fate will open up new ways of optimizing the design of synthetic ECM micro/nanotopography, which might be more effective than the natural one for differentiation control and stemness maintenance.…”

Section: Stem Cell Differentiationmentioning

confidence: 99%

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Miyoshi

Adachi

2014

Tissue Engineering Part B: Reviews

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“…14 Most studies showed that the surface topography on biomaterial scaffolds played an important role in regulating cell behaviors such as adhesion, proliferation, differentiation, cytoskeletal organization, apoptosis, and gene expression. [15][16][17] More importantly, our previous studies have reported that HA bioceramics with nanosheet, nanorod, and micro-nano-hybrid (the hybrid of nanorod and microrod) surfaces could be fabricated by hydrothermal reaction of the α-tricalcium phosphate (α-Ca 3 (PO 4 ) 2 , α-TCP) precursors in different aqueous solutions. In vitro studies showed that the nano-structured HA bioceramics could stimulate cell adhesion, proliferation, and osteogenic differentiation of BMSCs and adipose-derived stem cells (ASCs), while the mnHA achieved the best stimulatory effect.…”

Section: Introductionmentioning

confidence: 99%

“…17,19 After being cultured for 4 days, ALP activity in PDLSCs seeded on HA bioceramics with mnHA was significantly higher than that in the control sample. Moreover, ALP activity in the two groups peaked after 7 days, and a more significant difference appeared between the two groups.…”

mentioning

confidence: 96%

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

Mao¹,

Liu²,

Zhao³

et al. 2015

IJN

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The surface structure of bioceramic scaffolds is crucial for its bioactivity and osteoinductive ability, and in recent years, human periodontal ligament stem cells have been certified to possess high osteogenic and cementogenic differential ability. In the present study, hydroxyapatite (HA) bioceramics with micro-nano-hybrid surface (mnHA [the hybrid of nanorods and microrods]) were fabricated via hydrothermal reaction of the α-tricalcium phosphate granules as precursors in aqueous solution, and the effects of mnHA on the attachment, proliferation, osteogenic and cementogenic differentiations of human periodontal ligament stem cells as well as the related mechanisms were systematically investigated. The results showed that mnHA bioceramics could promote cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and expression of osteogenic/cementogenic-related markers including runt-related transcription factor 2 (Runx2), ALP, osteocalcin (OCN), cementum attachment protein (CAP), and cementum protein (CEMP) as compared to the HA bioceramics with flat and dense surface. Moreover, mnHA bioceramics stimulated gene expression of low-density lipoprotein receptor-related protein 5 ( LRP5 ) and β-catenin , which are the key genes of canonical Wnt signaling. Moreover, the stimulatory effect on ALP activity and osteogenic and cementogenic gene expression, including that of ALP, OCN, CAP, CEMP, and Runx2 of mnHA bioceramics could be repressed by canonical Wnt signaling inhibitor dickkopf1 (Dkk1). The results suggested that the HA bioceramics with mnHA could act as promising grafts for periodontal tissue regeneration.

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The use of combinatorial topographical libraries for the screening of enhanced osteogenic expression and mineralization

Cited by 117 publications

References 27 publications

An algorithm-based topographical biomaterials library to instruct cell fate

An algorithm-based topographical biomaterials library to instruct cell fate

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

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