The Use of Combined Laser Irradiation of the Intestinal Wall to Assess the Intestines Viability

Abstract

Resume. Aim: to study the informativeness of the scattering indicators of laser beams (LB) with wavelengths λ=0.63 and λ=0.4 µm for assessing the intestinal viability. Material and methods. 20 white rats with small (10 rats) and large (10 rats) intestinal obstruction models. In 6 hours, the intestines were irradiated with LB. After measuring the width of the LB scattering zone, intestinal tissues were taken for histological examination. The examination data of 10 intact rats were the control. Results. The LB width scattering zone increases if viability is disrupted. But absolute parameters have local and individual variability. The use of the parameters ratio of the LB width scattering zone with different wavelengths eliminates this variability. The parameters of the ratio differ significantly, depending on the degree of viability disruption. Conclusions. 1. Morphological changes in the intestinal walls are associated with significant differences in the ratio parameters of the scattering zone width of laser beams with wavelengths of λ=0.63 and λ=0.4 µm. 2. Ratio parameters > 1.58 un are a marker of preserved viability, parameters within 1.4-1.35 un are a marker of morphological disorders without necrosis, parameters < 1.2 un are a marker of necrotic disorders.