The use of cutaneous microdialysis to measure substance P-induced histamine release in intact human skin in vivo

Petersen, Lars Jelstrup; Poulsen, Lars K.; Søndergaard, Jørgen; Skov, Per Stahl

doi:10.1016/0091-6749(94)90186-4

Cited by 86 publications

(86 citation statements)

References 25 publications

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“…The histamine content was determined by means of the HR-Test (RefLab, Copenhagen, Denmark) by adding 50 µl supernatant (triplicates) to the wells of fibreglass-coated microtitre plates. After incubation for 1 h at 37°C, the samples were analyzed fluorometrically according to the manufacturer [23]. Results were expressed as nanograms histamine per millilitre fluid.…”

Section: Methodsmentioning

confidence: 99%

Patients with Subjective Food Hypersensitivity: The Value of Analyzing Intestinal Permeability and Inflammation Markers in Gut Lavage Fluid

Arslan

Kahrs²,

Lind³

et al. 2004

Digestion

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Background/Aim: Subjective food hypersensitivity is prevalent in the general population. The aim of this study was to seek objective evidence of food hypersensitivity by analyzing intestinal permeability and inflammation markers in gut lavage fluid. Methods: Fifty-two patients with abdominal complaints self-attributed to food hypersensitivity were examined by skin prick test, serum IgE analysis, double-blind, placebo-controlled food challenge (DBPCFC), and intestinal lavage. The results were compared with those of 44 patients without food hypersensitivity. Neither the patients nor the controls had organic diseases that could explain their symptoms. Intestinal lavage was performed by administering 2 liters of isotonic polyethylene glycol (molecular weight 3,350 daltons) solution containing 50 µCi of [⁵¹Cr]EDTA through a nasoduodenal tube. The first clear fluid passed per rectum was collected and analyzed for histamine, eosinophilic cationic protein (ECP), tryptase, and calprotectin. The intestinal permeability was assessed by determining the 5-hour urinary excretion of [⁵¹Cr]EDTA. Calprotectin was also analyzed in native faecal samples. Results: The ECP concentration in gut lavage fluid was significantly higher in the patients than in the controls (p = 0.007), but the overlap between groups was large. Food hypersensitivity was confirmed by positive DBPCFC in only 4 patients. On average, histamine and ECP concentrations were high in these patients. Tryptase, intestinal permeability, and faecal and lavage calprotectin levels were normal. Conclusions: Very few patients had objective evidence of food hypersensitivity. Analyzing intestinal permeability and inflammation markers in gut lavage fluid did not contribute to the diagnosis, but further studies on histamine and ECP are warranted.

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Section: Methodsmentioning

confidence: 99%

Patients with Subjective Food Hypersensitivity: The Value of Analyzing Intestinal Permeability and Inflammation Markers in Gut Lavage Fluid

Arslan

Kahrs²,

Lind³

et al. 2004

Digestion

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“…It was used initially for the recovery of neurotransmitters from the brains of experimental animals (Bito et al, 1966). Since then it has been adapted for use in many other tissues including skin (Petersen et al, 1994;Andersson et al, 1995;Krogstad et al, 1996;Kellogg et al, 1998;Clough, 1999), adipose tissue (Lindberger et al, 2001), muscle (Tegeder et al, 1999;Newman et al, 2001) and the gastrointestinal tract (Iversen et al, 1997). It has been used to assess the transcutaneous delivery of xenobiotics and their pharmacokinetic disposition, and to explore the efficacy of percutaneous delivery devices such as iontophoresis (Stagni et al, 1999).…”

mentioning

confidence: 99%

“…It has been used to assess the transcutaneous delivery of xenobiotics and their pharmacokinetic disposition, and to explore the efficacy of percutaneous delivery devices such as iontophoresis (Stagni et al, 1999). Microdialysis has also provided information about the physiology of the tissue space including the release of endogenous vasoactive molecules such as histamine (Petersen et al, 1994), nitric oxide (Clough, 1999), eicosanoids (Rhodes et al, 2001), and neuropeptides (Schmelz et al, 1997) under basal conditions and following dermal provocation. Microdialysis has advantages over other sampling techniques in that it is minimally invasive and is well tolerated by human volunteers.…”

mentioning

confidence: 99%

Effects of Blood Flow on the in Vivo Recovery of a Small Diffusible Molecule by Microdialysis in Human Skin

Clough¹,

Boutsiouki²,

Church³

et al. 2002

J Pharmacol Exp Ther

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The aim of this study was to investigate the impact of changes in local blood flow on the recovery of a small, diffusible molecule (sodium fluorescein) from the extravascular tissue space of the skin, by microdialysis in vivo. Loss and recovery of fluorescein by linear microdialysis probes (5-kDa molecular mass cutoff, 0.2 mm diameter) inserted 1 mm apart in pairs, at three sites in the skin of the volar surface of the forearm of healthy volunteers, was measured under conditions of basal, reduced (noradrenaline, 0.005 mg/ml), and increased (glyceryl trinitrate, patch) blood flow. Whereas loss of tracer from the delivery probe appeared unaffected by changes in local blood flow, retrieval of fluorescein by the second probe was directly related to blood flux, measured using scanning laser Doppler imaging. Steady-state recovery at vasoconstricted sites was 4.0 Ϯ 0.7 g ⅐ ml Ϫ1 compared with 1.8 Ϯ 0.7 g ⅐ ml Ϫ1 at control sites (p Ͻ 0.001). Local vasodilatation reduced the retrieval of fluorescein by ϳ50% to give a steadystate concentration of fluorescein in the dialysate at 40 to 50 min after the start of perfusion of 0.9 Ϯ 0.3 g ⅐ ml Ϫ1 (p ϭ 0.05). These studies in the skin are consistent with microdialysis theory. They suggest that clearance of solute by the blood will have a significant impact on microdialysis probe recovery and that, in the skin, the magnitude of this clearance is directly related to blood flow.Microdialysis is a well established technique for continuous sampling of small, water-soluble molecules within the extracellular fluid space in vivo. It was used initially for the recovery of neurotransmitters from the brains of experimental animals (Bito et al., 1966). Since then it has been adapted for use in many other tissues including skin (Petersen et al

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“…Furthermore, with microdialysis, release of inflammatory mediators in response to substance P infusion has been determined in skin (Petersen et al 1994). In the present study, microdialysis in combination with the 133 Xe washout blood flow technique was used to determine in vivo metabolic and inflammatory concentration changes in, as well as to calculate substrate output from, the peritendinous area just ventral to the human Achilles tendon at rest and during physical loading.…”

mentioning

confidence: 99%

Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans

Langberg

Skovgaard

Karamouzis

et al. 1999

The Journal of Physiology

157

152

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The use of cutaneous microdialysis to measure substance P-induced histamine release in intact human skin in vivo

Cited by 86 publications

References 25 publications

Patients with Subjective Food Hypersensitivity: The Value of Analyzing Intestinal Permeability and Inflammation Markers in Gut Lavage Fluid

Patients with Subjective Food Hypersensitivity: The Value of Analyzing Intestinal Permeability and Inflammation Markers in Gut Lavage Fluid

Effects of Blood Flow on the in Vivo Recovery of a Small Diffusible Molecule by Microdialysis in Human Skin

Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans

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