The teaching of instrumentation has consistently been the subject of undergraduate program in radiological technology (RT). However, opportunities for students to engage in face-to-face operation of linear accelerator machine (LINAC) during their education and training processes have been limited by financial and timeframe considerations. So, the aim of this study is to integrate a 3D-printed model and assess its effectiveness in teaching LINAC to RT undergraduate students. For this purpose, a LINAC physical model was developed using a fused deposition modeling 3D printer, with access through free online software. Color coding was implemented alongside with explanatory color cards to enhance understanding. A total of 114 participants were randomly divided into two groups: a control group and a 3D model group. Compare the theoretical scores of the 3D model group were found to be significantly higher than those of the control group, with p-value < 0.05. Moreover, the increase in student opportunity for hand-on training prior to their apprenticeships allowed participants in the 3D model group to reduced anxiety and better performance in clinical situation. To summarize participant feedback regarding the integration of this novel 3D technology in the context of LINAC learning and training. Results indicated that over 94% of students perceived the alternative teaching method as essential for enhancing both of their understanding and practical performance. In conclusion, the use of modern 3D-printed models technology, presents a potential tool for stimulating teaching and training activities, thereby improving overall educational outcomes in RT programs.