The use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)

Pérez-Atehortúa, Maritza; Galuppo, Andrea Giannotti; Rodrigues, Rômulo Batista; França, Thales de Souza; Teixeira, Nathalia dos Santos; Freitas, Thaiza Rodrigues de; Marques, Lis Santos; Gomes, Itamar Cossina; Benato, Jhony Lisbôa; Flores, Thales; Sanches, Eduardo Antônio; Souza, Ana R. Seabra de; Bustamante, Iván; Quirino, Monike; Bortolozzo, Fernando Pandolfo; Streit, Danilo Pedro

doi:10.1016/j.aquaculture.2022.738072

Cited by 6 publications

(12 citation statements)

References 42 publications

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“…It is likely that the difference in the range of temperature evaluated was not enough to lead to drastic impairment of the sperm quality. It is important to stress that the best indicators of sperm quality are not necessarily the motility nor the ability to fertilize an oocyte, but it also relies on the capacity to contribute in the obtention of a high percentage of live and hatched embryos (Pérez‐Atehortúa et al ., 2022). Therefore, future studies should also include this parameter to ensure that sperm quality is not affected by such an increase in temperature.…”

Section: Discussionmentioning

confidence: 99%

A simulated marine heatwave impacts European sea bass sperm quantity, but not quality

Geffroy

Sandoval‐Vargas

Boyer-Clavel

et al. 2023

Journal of Fish Biology

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Rapid environmental changes will be the major challenge that most biota will have to deal with in the near future. Extreme events, such as marine heatwaves, are becoming more frequent and could be spatially uniform at a regional scale for a relatively long period of time. To date, most research studies on heatwaves have focused on sessile organisms, but these extreme events can also impact mobile species. Here, a 3‐week marine heatwave was simulated to investigate its effects on the male reproductive performance of a Mediterranean Sea emblematic species, the European sea bass Dicentrarchus labrax. Males from the control condition (c. 13°C) produced significantly more sperm than those exposed to a relatively warm thermal treatment (c. 16°C). Nonetheless, neither the percentage of motile spermatozoa nor most of the other sperm motility parameters were significantly affected by the rearing temperature over the whole period. Overall, the results of this study suggest only moderated effects of a potential winter heatwave on the reproductive performance of male European sea bass.

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Section: Discussionmentioning

confidence: 99%

A simulated marine heatwave impacts European sea bass sperm quantity, but not quality

Geffroy

Sandoval‐Vargas

Boyer-Clavel

et al. 2023

Journal of Fish Biology

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“…During the process of sperm washing, freezing, and thawing, in order to obtain a high concentration of quality spermatozoa, it is critical that we reduce and remove any risk or chance of pathogen contamination during the preservation of frozen sperm in tanks. While sperm washing is usually performed before cryopreservation of mammalian sperm [164], in sperm washing studies applied in fish, since 2010, the washing process is performed after thawing [165][166][167]. In some species, it is used to separate seminal plasma from cells by centrifugation before freezing [168].…”

Section: Sperm Washing and Cell Separating Methodsmentioning

confidence: 99%

“…In studies using the density gradient centrifugation method, percoll was the most widely used gradient-forming chemical [169,170]. Sil-Select Plus TM [155] and AllGrad® 90% [167], which are commercial solutions produced in humans, have been tried in fish and successful results have been obtained.…”

Section: Sperm Washing and Cell Separating Methodsmentioning

confidence: 99%

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

Ekici¹,

Yamaner²,

Demircan³

2023

Biomedical Engineering

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In this section, cryopreservation of fish genetic resources, which is one of the important applications to ensure the sustainability of genetic resources of freshwater fish species, is discussed. At the same time, information is provided about the possible sources of contamination that may be encountered during cryopreservation applications. In this context, the results of sperm, egg, and embryo cryopreservation studies of fish and their success and failure in applications were evaluated in addition to the process from past to present. Information is given about the contamination that may develop depending on the applications in the process of cryopreservation and dissolving processes, as well as the studies carried out to eliminate extracellular disease agents. In the section, in addition to the evaluation of the results of scientific studies, commercial companies that commercially carry out gamete cryopreservation applications are also included. The contamination that may develop depending on the applications in the process of cryopreservation and thawing processes, as well as the studies carried out to eliminate extracellular disease agents are mentioned.

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“…However, comparing the integrity of the sperm membrane of the two treatments, we did not observe a difference. The same was presented when silver catfish post-thaw sperm was diluted in Ginsburg solution and centrifuged (Pérez-Atehortúa et al, 2022). Hence, the sperm membrane of the silver catfish is resistant to osmotic shock, as proven by post-thaw dilution.…”

Section: Discussionmentioning

confidence: 67%

“…Our results did not show a difference in mitochondrial activity between treatments. On the other hand, another study by our group (Pérez-Atehortúa et al, 2022) showed greater mitochondrial activity of post-thaw sperm diluted compared to undiluted. The difference between the results found in the studies may be due to the use of an extender containing other salts besides NaCl and a sperm pool.…”

Section: Discussionmentioning

confidence: 76%

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

de Souza França

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Fish sperm cryopreservation is a technique that can increase the reproduction in captive efficiency of freshwater and marine fishes. The technique is applied in environmental conservation programs and aquaculture. Over the last few decades, protocols for sperm cryopreservation from many fishes have been established. However, the researchers' main focus was successfully freezing and thawing sperm, neglecting the period in which the gametes remain in contact with the cryoprotective solution until fertilization. Exposure of sperm to cryoprotectant solutions after thawing can be harmful to the quality of the gametes since they can be toxic.The vast majority of the established protocols use ultra-resistant plastic containers to store sperm during the cryopreservation process. These containers usually are not reused, generating highly polluting waste for the environment. Furthermore, in some countries, the containers usually used are sold by a few industries, which makes acquisition difficult and increases the product's price. Thus, the main objective of the thesis was to create and test low-cost methodologies that enhance the use of fish post-thaw sperm and make the sperm cryopreservation process more environmentally friendly.The experiments in the Chapters 1 and 2 were developed in Brazil. We used the South American silver catfish Rhamdia quelen, a species considered an experimental model for native South American fishes. In vitro fertilization and sperm cryopreservation protocols for the species were already established; thus, we based our work on these protocols to apply innovations.In Chapter 1, we tested the use of post-thawing dilution to reduce the toxicity of the cryoprotectant solution. This technique is commonly used in mammalian sperm cryopreservation protocols but has never before been applied to post-thaw sperm of South American fishes. South American silver catfish post-thaw sperm samples were diluted in a saline extender (1.1% NaCl -325 mOsm kg -1 ; pH 7.6). The post-thaw sperm diluted samples showed higher velocities, straightness, progression, and flagellar beat frequency than SUMMARY 2 the cells of undiluted samples (control). The post-thawing dilution also provided higher fertilization and hatching rates than the control group. Thus, the post-thawing sperm dilution proved to be a simple, cheap, and efficient methodology that should be included in the silver catfish sperm cryopreservation protocol.

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The use of differential separation and density gradient with AllGrad 90% after thawing improves the sperm quality of South American catfish (Rhamdia quelen)

Cited by 6 publications

References 42 publications

A simulated marine heatwave impacts European sea bass sperm quantity, but not quality

A simulated marine heatwave impacts European sea bass sperm quantity, but not quality

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

Development and application of low-cost and environment-friendly techniques for fish sperm cryopreservation

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