The use of discontinuous Percoll gradients to separate populations of cells from human bone marrow and peripheral blood

Ellis, W.M.; Georgiou, George; Roberton, Don; Johnson, Gregory R.

doi:10.1016/0022-1759(84)90242-4

Cited by 38 publications

(19 citation statements)

References 11 publications

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“…Bone marrow-derived stem cells (BMSCs) are composed of a diverse cell population including mononuclear cells [36], MSCs [37], hematopoietic stem cells [38], side population cells [39] and very small embryonic-like stem cells [40–43]. With this diverse population of stem cells originating in bone marrow, a wide variety of approaches have been implemented for isolation and expansion [44–46] as well as variation in number of cells injected, means of delivery, delivery post injury, type of injury, and means of follow-up screening [47–50].…”

Section: Current Status Of Adult Stem Cell Therapiesmentioning

confidence: 99%

Empowering Adult Stem Cells for Myocardial Regeneration V2.0

Broughton

Sussman

2016

Circulation Research

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Much has changed since our survey of the landscape for myocardial regeneration powered by adult stem cells four years ago (Mohsin et al., Empowering adult stem cells for myocardial regeneration. Circ Res. 2011; 109(12):1415–1428) [1]. The intervening years since that first review has witnessed an explosive expansion of studies that advance both understanding and implementation of adult stem cells in promoting myocardial repair. Painstaking research from innumerable laboratories throughout the world is prying open doors that may lead to restoration of myocardial structure and function in the wake of pathologic injury. This global effort has produced deeper mechanistic comprehension coupled with an evolving appreciation for the complexity of myocardial regeneration in the adult context. Undaunted by both known and (as yet) unknown challenges, pursuit of myocardial regenerative medicine mediated by adult stem cell therapy has gathered momentum fueled by tantalizing clues and visionary goals. This concise review takes a somewhat different perspective than our initial treatise, taking stock of the business sector that has become an integral part of the field while concurrently updating “state of affairs” in cutting edge research. Looking retrospectively at advancement over the years as all reviews eventually must, the fundamental lesson to be learned is best explained by Jonatan Mårtensson: “Success will never be a big step in the future. Success is a small step taken just now.”

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Section: Current Status Of Adult Stem Cell Therapiesmentioning

confidence: 99%

Empowering Adult Stem Cells for Myocardial Regeneration V2.0

Broughton

Sussman

2016

Circulation Research

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“…Platelet-enriched pellets were obtained according to standard laboratory procedures. White blood cells were recovered as described by Ellis et al [24]. The final pellet, containing mononuclear cells, was incubated in tissue culture dishes containing Dulbecco's modified Eagle's medium with 10% serum, 100 U/ml penicillin, 100 Ìg/ml streptomycin and 0.25 Ìg/ml fungizone at pH 7.25.…”

Section: Isolation Of Blood Cellsmentioning

confidence: 99%

Blood Cells Cholinesterase Activity in Early Stage Alzheimer’s Disease and Vascular Dementia

Bernhardi

Alarcón

Mezzano

et al. 2005

Dement Geriatr Cogn Disord

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Blood acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities have been studied as markers for Alzheimer’s disease (AD), but their usefulness as a disease marker is controversial. To determine cholinesterase (ChE) activity during AD progression and whether ChE changes associate to other dementias, ChE activity was measured in lymphocytes, erythrocytes and platelets. Subjects underwent extensive medical and neuropsychological examination. Both early-AD and AD patients had lower AChE activity in lymphocytes compared to control subjects (p < 0.0001). In contrast, erythrocyte AChE activity was higher in patients with vascular dementia (p = 0.004). Low ChE activity in lymphocytes was the best discriminator for AD. Because it was already low at very early stages of AD, ChE could be helpful as an early biomarker of differential diagnosis for the follow-up of patients during their early stages of cognitive impairment before a clinical dementia is established.

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“…Different results were obtained although a general agree- ment was reached that NRBCs are distributed both in the light and in the heavy portion of the gradient and that no difference exists in the density distribution profile of fetal and adult NRBCs. Since most nucleated cells present in human blood are lighter than 1.090 [28], the introduction of a very high density cut like 1.109 or 1.119 g/ml yields indeed most fetal cells present in maternal blood but without any enrichment, since all other nucleated cells are equally retrieved.…”

Section: Discussionmentioning

confidence: 98%