The Use of Dried Blood Spots on Filter Paper for the Diagnosis of Hiv-1 in Infants Born to Hiv Seropositive Women

Jacob, Saramma Mini; Durairaj, Anitha; Vishwanath, R.; Parameshwari, S; Nm, Samuel

doi:10.1016/s0255-0857(21)01998-8

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Without access to life saving drugs (antiretroviral therapy and cotrimoxazole prophylaxix) about one third of infants die by age 1 year and 50% by age 2 years. Moreover the asymptomatics, below 18 months are missed out on prevention and treatment [4] Standard HIV antibody testing done in adults cannot identify infected infants in their first year of life due to maternal antibodies which are transferred to the baby during pregnancy [5] Hence This requires a virological test for diagniosing infants. Virological testing detects HIV DNA/RNA.HIV DNA testing can also be reliably performed on specimens collected onto filter paper dried blood spots.…”

Section: Discussionmentioning

confidence: 99%

“…Affordable ART and treatment are becoming increasingly available but is of less benefit in infants unless they can be diagnosed early. Most widely available serological assays used for adult pose difficulty in interpretation in infants aged under 9-12 months owing to passage of maternal antibodies [5] Early diagnosis of HIV allows health care providers to offer optimal care and treatment of HIV exposed infants and children, assists in decision making on infant feeding choices and avoids needless stress in mothers and families. Virological testing is therefore a required method of diagnosis of HIV in infancy.…”

Section: Introductionmentioning

confidence: 99%

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Early infant diagnosis (EID): A vital component of prevention of parent to child transmission program

Thakur¹,

Singh²,

Ghanghoriya³

et al. 2018

Int. J. Clin. Obstet. Gynaecol.

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Background: Early diagnosis of HIV infection in exposed infants and children is essential component to child survival. EID, a virological DNA PCR test on dried blood spot, aims at early diagnosis and ART initiation, breast feeding choices and linkage to care. It confers substantial benefit to the infected and uninfected infants and provides a unique opportunity to evaluate the performance of PPTCT program. Materials and method: Present study conducted in the department of Obstetrics and Gynecology, NSCB Medical College, Jabalpur from March 2016 to August 2017. HIV exposed infants and children between 6 weeks to 18 months of age were included. HIV testing (DNA PCR and antibody tests) done at 6weeks, 6, 12 and 18 months as per pediatric ART guidelines of NACO, 2015. Results: Total 145 infants/children were tested during study period. 8 found to be HIV positive which gives an incidence of 5.51% in out institute.112 (80.7%) had first visit between 6 weeks to 6 months.130(89.6)% mother-baby pair received ART/ARV prophylaxis. Transmission rate lowest (0.76%) in Mother-baby pair who received ART. 69(47.9% infants were on replacement feeding. The transmission rate was (8.1%). One death observed and 12 (8.27%) lost to follow up. EID results at first contact were accurate and well correlated with the results at 6, 12 and 18 month. Conclusion: EID is a vital component of PPTCT program. It confirms the diagnosis as early as 6 weeks and confers an accurate and consistent results throughout testing protocol, thus establishing the integrity of EID. It assists in decision making on infant feeding practices.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%