The use of effector gene based-markers to facilitate identification of Fusarium sp. infected shallot in Java, Indonesia

Abstract

Abstract. Herlina L, Istiaji B. 2020. The use of effector gene based-markers to facilitate identification of Fusarium sp. infected shallot in Java, Indonesia. Biodiversitas 21: 4677-4685. One of the most important diseases and become challenge in breeding resistant variety of shallots in Indonesia is Fusarium disease, caused by Fusarium oxysporum f.sp. cepae (FOC). To discriminate the F. oxysporum into forma speciales is uneasy, often managed through laborious and time-intensive disease assays., therefore molecular approaches become the most relevant choice. FOCs from Indonesia were examined by molecular criteria based on putative and effector genes-based markers, i.e.: SIX-genes, C5-gene, and CRX1-2-genes. The alignment analysis discovered some regions which sequences highly contained conserved bases, while amplification result bands vary in size, between 400 - 500 bp. The combined of 7 SIX-genes primers and 3 effector primers (C5 and CRX1-2) used in the clustering analysis of 15 FOC isolates in this study showed that they succeeded in separating 15 FOC isolates into 4 groups through NTSys. Clustering analysis showed that those markers succeeded in grouping 15 FOC isolates into different clades (by coefficient of similarity: 0.69). Phylogenetic analysis based on CRX genes sequence as putative effector genes confirmed that CRX1 and CRX2 genes were able to classify the FOC into their forma speciales. Those effectors genes are potential to serve as marker-templates to facilitate identification of FOC which infested shallots in Indonesia.

Suppression of Moler Disease on Shallot by Ziram Fungicide in South Sumatera Lowland

“…The final symptoms of an attack of this disease cause plant death (Figure 1). Moler disease is caused by Fusarium oxysporum [10][10][10][10][10] [10]. Fusarium oxysporum is a pathogen that infects the roots and vascular parts of the plant which further spreads to the upper tissues and blocking the xylem vessels [11].…”

The Causal Agent of Fusarium Disease Infested Shallots in Java Islands of Indonesia

“…To date, there is a very limited reference and information for any source of shallot as germplasms which resistant against Fusarium disease. As the increase of the importance of the disease in the field, the study of response genotype of shallot to pathogenic FOC infection becomes important and very relevant to carry out in order to validate or confirm the information of the existence of resistance or tolerant cultivar or a variety of shallots [5].This research is aimed to determine the disease incidence, disease severity, and criteria of resistance to shallots in Indonesia by trying to develop our methods which are compiled based on various reference methods of determining disease scoring developed by other researchers. Also aimed to investigate the several shallots genotype response against several isolates of FOC.…”