The Use of Emulsification Technologies to Enhance Rapeseed Oil Consumption During Industrial Streptomyces rimosus Fed-batch Fermentations

Hewitt, Christopher J.; Papapanagiotou, P.A.; Quinn, Henry; Molitor, J.P.; Stocks, Stuart M.; Nienow, Alvin W.

doi:10.1205/cherd06236

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Additionally, the different evaporation rates may lead to different viscosities of the culture broth. Indeed, Hewitt et al [24] used self-emulsifying method to increase oil utilization during industrial fed-batch process. At the same time, the broth viscosity decreased markedly.…”

Section: Biomass Accumulation and Penicillin G Yield In Both Pilot Anmentioning

confidence: 99%

Phospholipid profiles of Penicillium chrysogenum in different scales of fermentations

Qiao

Cao

et al. 2013

Engineering in Life Sciences

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Optimizing industrial‐scale fermentation for Penicillium chrysogenum is of significance for increasing commercial production of antibiotics. Lipidomics could be a valuable tool for the investigation in the behaviors of P. chrysogenum in realistic industrial environments. In this work, the phospholipid (PL) profiles of an industrial strain of P. chrysogenum were compared in different scale processes. PL was performed on LC/ESI/MSn system. It was found that industrial P. chrysogenum cells absorbed significant amounts of exogenous saturated and (poly) unsaturated fatty acids (PUFAs) from feedstock and incorporated them into their cell membranes during two fermentations. Results showed that the PL species that contain the PUFAs, that is, linolenic acid and hexadecadienoic acid, were quite variable between pilot and industrial scales of fermentations. Higher levels of PUFA‐containing PLs in rapid‐ and linear‐growth stages during industrial fermentation implied the occurrence of dramatic variations in cell membrane fluidity during these periods. It was speculated that this behavior was due to impacts of multiple physical and chemical factors present in the fermentation environment during this large‐scale fed‐batch process. The identified PUFA‐containing PLs could be used as valuable biomarkers for optimizing industrial‐scale fermentation for P. chrysogenum.

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Section: Biomass Accumulation and Penicillin G Yield In Both Pilot Anmentioning

confidence: 99%

Phospholipid profiles of Penicillium chrysogenum in different scales of fermentations

Qiao

Cao

et al. 2013

Engineering in Life Sciences

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“…Thus, fedbatch strategy was considered as alternative cultivation process by many researchers to increase antibiotic production by actinomycetes such as in case of rifamycins production by Amycolatopsis mediterranei (El Enshasy et al 2003), clavulanic acid by S. clavuligerus (Saudagar and Singhal 2007), and daptomycin by S. roseosporus (Ng et al 2014). Papapanagiotou et al (2005) and Hewitt et al (2007) investigated the application of emulsification technologies to enhance rapeseed oil consumption during OTC under fed-batch conditions. However, they aimed mainly to compare between the effect of feeding normal rapeseed oil and rapeseed oil emulsion produced by phase inversion temperature on the utilization of rapeseed oil.…”

Section: Introductionmentioning

confidence: 99%

Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale

Elsayed

Omar

El‐Enshasy

2015

Braz. arch. biol. technol.

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Scale‐Up, Stirred Tank Reactors

Nienow

2010

Encyclopedia of Industrial Biotechnology

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Scale‐up is best approached by considering the need to provide the optimum environment for the cell. It is inevitably a compromise and needs appropriate small‐scale data as discussed here. However, arbitrary and inflexible “scale‐up rules” are not useful. The most critical aspects of scale‐up are oxygen and carbon dioxide mass transfer and their concentration in the broth, all of which depend on the air sparge rate and power input from the selected impeller(s). The Rushton turbine is discussed followed by the now‐preferred hollow‐blade and up‐pumping, high solidity ratio hydrofoils. Bulk blending/homogeneity (especially at the high aspect ratios found on the commercial scale), holdup, and foaming and heat transfer in turbulent conditions as found with small‐sized microorganisms are considered, followed by the additional complexity that arises with these parameters when dealing with high viscosity, rheologically complex broths with mycelial and biopolymer fermentations. The impact of scale‐up on two aspects of bioperformance is considered, namely, mechanical stress from agitation and stresses from the inevitable poorer homogeneity. It is concluded that mechanical stresses are only important with mycelial fermentations and the impact depends on the organism. Stresses due to spatial differences in dO 2 and in pH batch fermentations, as well as nutrients in fed batch, cause a poorer performance at the commercial scale. Such information is vital for enabling a realistic appreciation beforehand of the likely large‐scale bioperformance, and for guiding design. Finally, with the present understanding of the physical and biological aspects of bioprocesses, computational fluid dynamics (CFD) is unlikely to be able to significantly improve it.

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The Use of Emulsification Technologies to Enhance Rapeseed Oil Consumption During Industrial Streptomyces rimosus Fed-batch Fermentations

Cited by 3 publications

References 19 publications

Phospholipid profiles of Penicillium chrysogenum in different scales of fermentations

Phospholipid profiles of Penicillium chrysogenum in different scales of fermentations

Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale

Scale‐Up, Stirred Tank Reactors

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