The use of enzyme-linked immunosorbent assay for the quantitation of Calloselasma rhodostoma (Malayan pit viper) venom and venom antibodies

Tan, Nget-Hong; Yeo, Kei-Huat; Jaafar, Mohd Iskandar Nik

doi:10.1016/0041-0101(92)90033-2

Cited by 11 publications

(2 citation statements)

References 15 publications

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“…In our study, species-specific anti-Pf antivenom was highly efficient in discrimination of Pf venom in double-sandwich and indirect ELISA irrespective of an unavoidable persistent background frequently encountered with ELISA assays (Ho et al, 1986). Double sandwich ELISA, being able to detect antigens with several epitopes, at least two (Tan et al, 1992), exhibits much higher specificity than the indirect ELISA. This was well demonstrated in our work, additionally, the presence of NH4SCN exaggerates such specificity to high levels ( Fig.…”

Section: Discussionmentioning

confidence: 67%

Antigenic cross-reactivity and species-specific identification of Pseudocerastes persicus fieldi snake venom

Ibrahim

El-Kady

2016

Toxicon

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Section: Discussionmentioning

confidence: 67%

Antigenic cross-reactivity and species-specific identification of Pseudocerastes persicus fieldi snake venom

Ibrahim

El-Kady

2016

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“…The indirect ELISA and double sandwich procedures were performed according to a previous description (14). The LD 50 value was calculated according to Weil (15).…”

Section: Bungarus Flaviceps Bungarus Fasciatus Bungarus Candidus (Mmentioning

confidence: 99%

Enzymatic and immunological properties of Bungarus flaviceps (red-headed krait) venom

Tan¹,

Fung²,

Ponnudurai³

2010

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:Bungarus flaviceps (red-headed krait) venom presents an intravenous LD 50 of 0.32 μg/g and exhibits enzymatic activities similar to other Bungarus toxins. ELISA cross-reactions between anti-Bungarus flaviceps and a variety of elapid and viperid venoms were observed in the current study. Double-sandwich ELISA was highly specific, since anti-B. flaviceps serum did not cross-react with any tested venom, indicating that this assay can be used for species diagnosis in B. flaviceps bites. In the indirect ELISA, anti-B. flaviceps serum cross-reacted moderately with three different Bungarus venoms (9-18%) and Notechis scutatus venom, but minimally with other elapid and viperid toxins. The results indicated that B. flaviceps venom shares common epitopes with other Bungarus species as well as with N. scutatus. The lethality of the B. flaviceps venom was neutralized effectively by antiserum prepared against B. candidus and B. flaviceps toxins and a commercial bivalent elapid antivenom prepared against B. multicinctus and Naja naja atra venoms, but was not neutralized by commercial antivenoms prepared against Thai cobra, king cobra and banded krait. These data also suggested that the major lethal toxins of B. flaviceps venom are similar to those found in B. multicinctus and B. candidus venoms.

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Nachweis des Nelkenadernscheckungsvirus (CVMV) mit verschiedenen ELISA-Systemen

Messerschmid

Sarkar

1995

Anz. Schadlingskde., Pflanzenschutz, Umweltschutz

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The use of enzyme-linked immunosorbent assay for the quantitation of Calloselasma rhodostoma (Malayan pit viper) venom and venom antibodies

Cited by 11 publications

References 15 publications

Antigenic cross-reactivity and species-specific identification of Pseudocerastes persicus fieldi snake venom

Antigenic cross-reactivity and species-specific identification of Pseudocerastes persicus fieldi snake venom

Enzymatic and immunological properties of Bungarus flaviceps (red-headed krait) venom

Nachweis des Nelkenadernscheckungsvirus (CVMV) mit verschiedenen ELISA-Systemen

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