The use of eosin B to assess the viability and developmental potential of rat embryos

Dooley, Michael P.

doi:10.31274/rtd-180813-11124

Cited by 5 publications

(4 citation statements)

References 151 publications

(194 reference statements)

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“…The dead sperm were stained red, whereas the living sperm were not. 100 sperm cells were counted in each sperm sample, and the percentage of viable sperm was computed [ 21 ]. Agnihotri et al [ 22 ] used a technique to detect the mitochondrial membrane potential (MMP) of sperm.…”

Section: Methodsmentioning

confidence: 99%

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

Jannatifar,

Piroozmanesh,

Jalalabadi

et al. 2023

Anat Cell Biol

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Septin4 belong to a family of polymerizing GTP-binding proteins that are required for many cellular functions, such as membrane compartmentalization, vesicular trafficking, mitosis, and cytoskeletal remodeling. Since, Septin4 is expressed specifically in the testis, we aimed to determine the association between Septin4 gene expression with sperm quality, DNA damage, and stress oxidative level in infertile patients. The present study included 60 semen samples that grouped into three groups: normozoospermia (n=20), asthenozoospermia (n=20), astheno-teratozoospermia (n=20). Initially, semen parameters were analyzed by using the World Health Organization protocol. The mRNA expression of Septin4 in sperm was examined using reverse transcription-polymerase chain reaction. Oxidative stress markers, i.e., total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde, were determined by ELISA kit. The current study showed a statistically significant highly positive correlation in Septin4 gene expression with sperm motility, normal morphology, viability, capacity, and sperm mitochondrial membrane potential (MMP). However, it showed significant negative correlation with sperm DNA fragmentation. Septin4 had a significant correlation with stress oxidative factor and antioxidant enzyme levels. In conclusion, Septin4 gene expression provides clinical useful information for the diagnosis of male infertility. It might be a marker for discrimination between fertile and infertile patients. The current study showed a statistically significant highly positive correlation in Septin4 gene expression with sperm motility, normal morphology, viability, capacity, and sperm MMP. However, it shows significant negative correlation with sperm DNA fragmentation. Septin4 had a significant correlation with stress oxidative factor and antioxidant enzyme levels.

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Section: Methodsmentioning

confidence: 99%

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

Jannatifar,

Piroozmanesh,

Jalalabadi

et al. 2023

Anat Cell Biol

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“…Dye exclusion assays determine the viability of suspension samples, with nonviable samples appearing in blue cytoplasm and living samples appearing in clear cytoplasm. Trypan blue [ 9 , 10 , 11 , 12 , 13 , 14 ] is a toxic assay for mammalian cells, and eosin [ 13 , 15 , 16 , 17 ], congo red [ 18 ], and erythrosine B stain assays [ 19 , 20 ] are nontoxic assays for mammalian cells. The principle of fluorometric assays is based on cellular esterases’ cleavage of a nonfluorescent compound into a fluorescent compound.…”

Section: Living Sample Viability Measurement Methodsmentioning

confidence: 99%

Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion

Al-Madani

Yao

et al. 2022

Biosensors

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Living sample viability measurement is an extremely common process in medical, pharmaceutical, and biological fields, especially drug pharmacology and toxicology detection. Nowadays, there are a number of chemical, optical, and mechanical methods that have been developed in response to the growing demand for simple, rapid, accurate, and reliable real-time living sample viability assessment. In parallel, the development trend of viability measurement methods (VMMs) has increasingly shifted from traditional assays towards the innovative atomic force microscope (AFM) oscillating sensor method (referred to as nanomotion), which takes advantage of the adhesion of living samples to an oscillating surface. Herein, we provide a comprehensive review of the common VMMs, laying emphasis on their benefits and drawbacks, as well as evaluating the potential utility of VMMs. In addition, we discuss the nanomotion technique, focusing on its applications, sample attachment protocols, and result display methods. Furthermore, the challenges and future perspectives on nanomotion are commented on, mainly emphasizing scientific restrictions and development orientations.

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“…Fertile matings were based on the recovery of viable embryos from the reproductive tract of the female rat when euthanized on gestational day 3 or by the delivery of live offspring at term. Viability of embryos was determined according to methods of Dooley et al [1988].…”

Section: Reproductive Assessment Of Male Offspringmentioning

confidence: 99%

Neural and behavioral teratological evaluation of rats exposed to ultra-wideband electromagnetic fields

Cobb

Jauchem

Mason

et al. 2000

Bioelectromagnetics

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Several investigators have reported teratologic effects of electromagnetic field exposure. The majority of these studies have been performed at levels of exposure that could produce substantial heating of the animals. New and unique sources of ultra-wideband (UWB) electromagnetic fields are currently being developed and tested that are capable of generating nonthermalizing, high-peak-power, microwave (MW) pulses with nanosecond (ns) pulse widths, picosecond (ps) rise times, and an UWB of frequencies. Our study was performed to determine if teratological changes occur in rat pups as a result of (i) daily UWB exposures during gestation days 3-18, or (ii) as a result of both prenatal and postnatal (10 days) exposures. Dams were exposed either to (i) UWB irradiation from a Kentech system that emitted a 55 kV/m-peak E field, 300 ps rise time, and a 1.8 ns pulse width, average whole-body specific absorption rate 45 mW/kg; (ii) sham irradiation; or (iii) a positive control, lead (Pb) acetate solution (2000 microg/ml) continuously available in the drinking water. Offspring were examined for ontogeny (litter size, sex-ratios, weights, coat appearance, tooth-eruption, eye-opening, air-righting, and ultrasonic stress vocalizations). Male pups were tested on various performance measures (locomotor, water-maze learning, and fertilization capabilities). The pups postnatally exposed were examined for hippocampal morphology and operant behavior. Behavioral, functional, and morphological effects of UWB exposure were unremarkable with these exceptions: (i) The UWB-exposed pups emitted significantly more stress vocalizations than the sham-exposed pups; (ii) the medial-to-lateral length of the hippocampus was significantly longer in the UWB-exposed pups than in the sham-exposed animals; (iii) male offspring exposed in utero to UWB mated significantly less frequently than sham-exposed males, but when they did mate there was no difference in fertilization and offspring numbers from the sham group. There does not appear to be a unifying physiological or behavioral relationship among the significant differences observed, and our findings could be due to the expected spurious results derived when a large number of statistical comparisons are made. Significant effects found between our positive-controls and other groups on numerous measures indicates that the techniques used were sensitive enough to detect teratological effects. Bioelectromagnetics 21:524-537, 2000. Published 2000 Wiley-Liss, Inc.

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The use of eosin B to assess the viability and developmental potential of rat embryos

Cited by 5 publications

References 151 publications

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients

Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion

Neural and behavioral teratological evaluation of rats exposed to ultra-wideband electromagnetic fields

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