The use of extracted teeth for in vitro bonding studies: A review of infection control considerations

DeWald, Janice P.

doi:10.1016/s0109-5641(97)80015-2

Cited by 112 publications

(109 citation statements)

References 35 publications

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“…In dental research, several sterilization methods are common including autoclaving, chemical heat or dry heat sterilization. However, they might either affect material properties of the teeth or they are unsuitable for amalgam-containing teeth [1].…”

Section: Introductionmentioning

confidence: 99%

Effect of sterilization by gamma radiation on nano-mechanical properties of teeth

Brauer¹,

Saeki²,

Hilton

et al. 2008

Dental Materials

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Objectives. Extracted teeth used in dental research need to be considered infective and hence be sterilized without the materials properties being altered. This study examined the effect of gamma radiation on the nano-mechanical properties of dentin and enamel of extracted human third molars.Methods. Whole teeth were sterilized using gamma radiation doses of 7 kGy and 35 kGy, respectively; teeth of the control group were not treated with gamma radiation. Crowns were sectioned occlusally and polished. Elastic modulus and hardness were tested using atomic force microscopy with nano-indentations under wet conditions.Results. We found no significant dose-response relationship in elastic modulus or hardness in either dentin or enamel.Significance. Nano-indentation is a common technique for the determination of local mechanical properties in biological hard tissues. Gamma radiation is an efficient way to sterilize extracted teeth while alterations of dentin and enamel mechanical properties are minimized.

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Section: Introductionmentioning

confidence: 99%

Effect of sterilization by gamma radiation on nano-mechanical properties of teeth

Brauer¹,

Saeki²,

Hilton

et al. 2008

Dental Materials

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“…Results of previous studies have indicated that this method does not affect the permeability of dentin or cause structural alterations 9,11 . However, gamma radiation is an expensive and complex technique that is not easily accessible 19 .…”

Section: Discussionmentioning

confidence: 96%

“…The results of this research are in agreement with those obtained in previous studies that indicated the capacity of this method to deactivate different microorganisms, such as viruses, fungi and spores 2,[8][9] . However, a number of previous studies have stated that the method has disadvantages, which are related to the maintenance of the structural properties of extracted teeth 11,[18][19] . Although the NaF 2% solution did not sterilize the specimens, the microbial load was reduced after seven and 14 days of treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Among the effective chemical and physical methods, formalin solution (10%) and autoclave are easy-to-use, inexpensive and adequate sterilizers for routine use 9 . However, characteristics such as the potential for irritation and highly carcinogenic qualities 10 as well as alterations in the structure of dentin 11 , are disadvantages that have led to the need for an adequate alternative disinfectant for extracted teeth.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of sodium fluoride 2% as a new method of disinfecting extracted human teeth

Carvalho

Santos

Watanabe

et al. 2016

RGO, Rev. Gaúch. Odontol.

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“…Several sterilization methods including autoclaving, chemical heat or dry heat sterilization are common used. However, they might either affect the material properties of the teeth or they are unsuitable for teeth containing amalgam (DeWald, 1997).…”

Section: Introductionmentioning

confidence: 99%

Structural and Dielectrical Properties of Sterilized Human Teeth

Eissa¹

2012

Physics International

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Problem statement:This study investigated the changes in crystallinity and dielectrical properties (conductivity) that could be demonstrated in extracted human teeth (enamel and dentin) subjected to γ-radiation at the sterilization dose (25 kGy). Approach: Twenty human third molar teeth were used in the study. Teeth were prepared so forty enamel and dentin samples were obtained. The samples were divided into two groups. The first group contains twenty samples of enamels. The second ones contains twenty samples of dentins. Each group was further subdivided into two subgroups (10 samples each), one for control while the other for irradiated samples. The X-ray Diffraction Pattern (XDR) studies for the control and irradiated enamel and dentin samples were carried out by X-ray diffractometer. In addition, the microhardness test for the present samples were investigated. Results: The crystallinity in irradiated enamel samples was better than that in dentin where the average crystallite size in enamel was larger than that in dentin. Also, it was found that the dielectrical conductivity for dentin increased with frequency after exposure to γ-radiation. Accordingly, the number and mobility of charge carriers were increased. Conclusion: Exposure of human teeth to gamma radiation at the sterilization dose decreases the hardness of both enamel and dentin.

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The use of extracted teeth for in vitro bonding studies: A review of infection control considerations

Cited by 112 publications

References 35 publications

Effect of sterilization by gamma radiation on nano-mechanical properties of teeth

Effect of sterilization by gamma radiation on nano-mechanical properties of teeth

Assessment of sodium fluoride 2% as a new method of disinfecting extracted human teeth

Structural and Dielectrical Properties of Sterilized Human Teeth

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