1961
DOI: 10.1111/j.1365-2141.1961.tb00346.x
|View full text |Cite
|
Sign up to set email alerts
|

The Use of Factor VIII Freed from Fibrinogen in the Treatment of Haemophilia Patients

Abstract: THE treatment of haeinophilic bleeding often requires the use of concentrated Factor VIII.Casillas, Simoiictti and Pavlovsky (1959) compared the available methods of separating Factor VIII from liurnan plasma and found that the technique of Blomback, Blombsck and Nilsson (1959) was the most satisfactory. The preparation made by this process (called FI-0)is still contaminated with many other proteins, notably fibrinogen, which hinder its concentration and the better characterization of the active component.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
3
0

Year Published

1963
1963
1976
1976

Publication Types

Select...
7
1

Relationship

1
7

Authors

Journals

citations
Cited by 10 publications
(3 citation statements)
references
References 8 publications
0
3
0
Order By: Relevance
“…Under the conditions of purifying platelet cofactor I from plasma, at this step of the procedure, a large amount of fibrinogen is present, which precipitates together with platelet cofactor I. The close relationship of fibrinogen and platelet cofactor I in attempts of purifying AHG from plasma has been noted by different investigators (5,7) and the removal of fibrinogen from AHG preparations seems to be a major problem (2,4,8,27).…”
mentioning
confidence: 91%
“…Under the conditions of purifying platelet cofactor I from plasma, at this step of the procedure, a large amount of fibrinogen is present, which precipitates together with platelet cofactor I. The close relationship of fibrinogen and platelet cofactor I in attempts of purifying AHG from plasma has been noted by different investigators (5,7) and the removal of fibrinogen from AHG preparations seems to be a major problem (2,4,8,27).…”
mentioning
confidence: 91%
“…DEAE-cellulose chromatography: 1 g of DEAE-cellulose DE-32 is equilibrated with 0.005 m sodium phosphate, 0.06 m NaCI and 0.5 m glycine, pH 7.2 [12] and packed in a suitable column. 1 ml of the above-described preparation of highly purified bovine factor VI11 is diluted with 9 ml of the equilibrating buffer and filtered through the column.…”
Section: Methodsmentioning
confidence: 99%
“…In hemophilia A this factor is supposed to be lacking. Its isolation from human and animal plasma has been tried by applying a variety of different purification methods [2,3,4,5,10,18]. Thus far only concentrates of antihemophilic factor have been obtained that are useful in the treatment of an acute bleeding episode in patients with hemophilia A.…”
mentioning
confidence: 99%