The use of flax (Linum usitatissimum) as a model system for studies on organogenesis in vitro: the effect of different carbohydrates

Abstract

Three carbohydrate sources, maltose, sucrose and cellobiose, were compared for the respective effects on regeneration from internodal sections of flax. It was found that different patterns of morphogenesis were attributable to the type of carbohydrate and its concentration. Mean shoot regeneration was highest on a medium containing 87 mM maltose with a rate of 2.4 shoots per explant and root formation was stimulated to the greatest degree on a medium with 87 mM sucrose. This medium also resulted in the highest tissue dry weight.

Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

“…Linum usitatissimum L. (flax/linseed) is an important crop as a source of oil and fibre in Europe and North America (Millam et al, 1992;Bartoðová, 2005). Flax has been widely used in biotechnology studies, including plant cell, tissue and organ culture, as it has a relatively short life-cycle, high regeneration capacity and small genome size (Bennet and Smith, 1976).…”

Explant position effect on gene transformation to flax (Linum usitatissimum L.) via Agrobacterium tumefaciens

“…Flax regenerates easily from hypocotyl segments under in vitro conditions (11,12,13,14,15). However, the recovery of transgenic flax plants (13,16).…”

Screening of Five and Six-Membered Nitrogen-Containing Heterocyclic Compounds as New Effective Stimulants of Linum Usitatissimum L. Organogenesis in Vitro

“…Data of our previous researches and numerous data of other authors witness that regeneration ability of flax (Linum usitatissimum L.) is under the influence of various types of growth regulators and their different concentrations in the culture media as well as origin of the plant explants [55,[62][63][64][65][66][75][76][77][78].…”

“…Because of using Linum usitatissimum L. in the biotechnology as an important source for bioactive compounds, numerous studies devoted to elaboration of effective techniques for in vitro regeneration of this plant have been conducted for many years [54,55,[61][62][63][64][65][66]. Nevertheless, the elaboration of new efficient methods for in vitro cultivation and regeneration of this plant is very actual problem today.…”