The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

Tabll, Ashraf A.; Ismail, Hisham

doi:10.5772/19384

Cited by 7 publications

(5 citation statements)

References 99 publications

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“…One of the major hallmarks of cancer is flawed DNA amplification cycle which leads to tumor progression (44). The DNA content of the cell lines was determined by performing ploidy analysis (45). Since normal lymphocytes rarely exhibit any altered DNA content (46) and their average DNA value has been designated as diploid (47), normal lymphocytes from a healthy donor were used as a diploid control for the experiment.…”

Section: Characterization Of Established Cell Linesmentioning

confidence: 99%

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

et al. 2022

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Oral tongue squamous cell carcinoma (OTSCC) is one of the major causes of fatality in India due to very high percentage of patients with habits of smoking and chewing tobacco and associated products. Being highly heterogeneous in nature, every patient poses a different challenge clinically. To understand disease progression in an improved way, knowledge of cross-talk between tumor stroma and the tumor cells becomes indispensable. Patient-derived in vitro cell line models are helpful to understand the complexity of diseases. However, they have very low efficiency of establishment from the tumor samples, particularly the cancer-associated fibroblasts (CAFs). In the present study, two novel autologous pairs were immortalized spontaneously from non-habitual, HPV-positive patients, who presented with OTSCC. The epithelial and fibroblast cell lines had typical polygonal and spindle-shaped morphology, respectively. Positive staining with epithelial specific Pan-cytokeratin (PanCK) and fibroblast specific protein (FSP-1) further confirmed their epithelial and fibroblast origin. Unique Short Tandem Repeat (STR) profile of the cultures confirmed their novelty, while the similarity of the STR profiles between the epithelial and fibroblast cells from the same patient, confirmed their autologous nature. DNA analysis revealed aneuploidy of the established cultures. An increase in the tumorigenic potential of the established epithelial cultures upon treatment with CAF-conditioned medium proved the 'CAF-ness' of the established fibroblast cells. The established cultures are the first of their kind which would serve as a useful platform in understanding the tumor-stroma cross-talk in tongue cancer progression.

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Section: Characterization Of Established Cell Linesmentioning

confidence: 99%

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

et al. 2022

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“…Using FCM to measure DNA content provides important information on cell distribution throughout the cell cycle phases (G0/G1, S-phase, and G2/M), disclosing the DNA ploidy of the studied cell population and calculating the frequency of apoptotic cells. Furthermore, it can measure a substantial number of separate cells in a short time (seconds to minutes) (Tabll and Ismail, 2012). El-Deftar et al, (2012) revealed that DNA ploidy was heterogeneous within the single tumor, and when several tissue samples from the same tumor were analyzed, the incidence of aneuploidy increased (El-Deftar et al, 2012).…”

Section: Author Contribution Statementmentioning

confidence: 99%

Could Argyrophilic Nucleolar Organizer Regions count mirror DNA Ploidy in Malignant Salivary Gland Tumors?

Hussien

Mohamed

Mansor

et al. 2022

Asian Pac J Cancer Prev

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Objective: Nucleolar organizer regions (NORs) are DNA coils that transcribe to ribosomal RNA. The NORassociated protein, termed argyrophilic NOR (AgNOR), was visible within the nucleus by staining with silver nitrate examination via the light microscope. AgNOR counting is a proliferation marker and may help in the diagnosis and prognosis of various neoplastic lesions. Aneuploidy (abnormal DNA content) can predict the progression, survival and prognosis of the tumors. The aim of this study was to evaluate the role of AgNORs, DNA ploidy status, and total S-phase fraction (TSPF) as prognostic parameters in malignant salivary gland tumors (MSGTs). Methods: The current study is a retrospective study on a cohort of MSGTs (N=47), to assess AgNORs using Silver Nitrate stain, DNA index (DI), and TSPF using flow cytometry (FCM). Data including tumor size and site, lymphovascular invasion (LVI), lymph node metastasis (LNM) were collected. Results: The AgNORs count was statistically significant with MSGT type. DI was found to have a significant association with tumor site, tumor size and MSGT type. In addition, TSPF was found to be significantly associated with LVI. A moderate positive correlation was noted between AgNORs count and TSPF. LNM, tumor site, high AgNORs and low DI were all associated with short disease-free survival (DFS) and poor overall survival (OS). Conclusion: The present study revealed that high AgNORs count, DNA aneuploidy and TSPF had a poor influence on MSGTs prognosis.

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“…These are supported by AO/PI staining showing the early apoptotic cells (chromatin condensation, nuclear fragmentation, and cell blebbing) and late apoptotic cells that were observed in MDA-MB-231 and HT-29 cells incubated with cashew shoots extract. This indicates that the cell apoptosis that leads the splitting of nuclear DNA into several fragments (19,30).…”

Section: Discussionmentioning

confidence: 99%

Phytochemicals Screening and Anti-proliferative Activities of Anacardium occidentale Shoot Extract in Breast Cancer Cells

Chan,

Shafie,

Meli

et al. 2023

MJMHS

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Introduction: Anacardium occidentale or cashew are popular traditional food and have raised research interest for complementary cancer treatment. Cancer has become leading cause of death and treatment involved severe side effects. In present study, we aim to study the anti-proliferative effects of cashew shoots in breast cancer (MDA-MB-231), colorectal cancer (HT-29) and liver cancer (HepG2) cell lines. Methods: Cell lines were treated with 70% ethanolic cashew extract for cytotoxicity test with MTT assay. AO/PI dual fluorescent assay and RNase/PI staining were used to determine apoptosis induction effects. Phytochemicals screening was carried out by using gas chromatography mass spectrometry (GCMS) and liquid chromatography mass spectrometry (LCMS). Results: The cytotoxicity assay of cashew shoot extract demonstrated IC50 of 81.1 ± 0.11 μg/ml for MDA-MB-231, 307.5 ± 2.31 μg/ml for HT-29 and 272.6 ± 1.91 μg/ml for HepG2 cell lines. The apoptotic bodies include chromatin condensation, cell blebbing and nuclear fragmentation and apoptosis induction were shown by AO/PI staining. There was significant increase of cell count in sub-G0 phase in MDA-MB-231 cell lines treated with cashew shoot extract. It was demonstrated that cashew shoot extract contained 38 compounds from GCMS such as sitosterol, tannin, pyrogallol, phenol and 20 compounds from LCMS such as citric acid, gallic acid, myricetin and hinokiflavone that may give rise to its anti-cancer effect. Conclusion: Cashew shoot extract demonstrated potential anti-cancer properties thus further study is required to investigate its mechanism as anti-cancer agent.

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The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

Cited by 7 publications

References 99 publications

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

Establishment and characterization of novel autologous pair cell lines from two Indian non‑habitual tongue carcinoma patients

Could Argyrophilic Nucleolar Organizer Regions count mirror DNA Ploidy in Malignant Salivary Gland Tumors?

Phytochemicals Screening and Anti-proliferative Activities of Anacardium occidentale Shoot Extract in Breast Cancer Cells

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