The use of fluorescein-conjugated Bandeiraea simplicifolia B4-isolectin as a histochemical reagent for the detection of α-d-galactopyranosyl groups

Peters, Barry P.; Goldstein, Irwin J.

doi:10.1016/0014-4827(79)90392-6

Cited by 185 publications

(91 citation statements)

References 35 publications

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“…Fluoresceinated-B4 isolectin was shown to bind in a specific manner to basement membrane structures in a variety of murine tissues [8]. We now believe that laminin is the co~astituent of basement membrane to which the B4 isolectin binds.…”

Section: Discussionmentioning

confidence: 82%

“…Of the a-galactosidase activity initially present in the digestion mixture 70% remained after 94 h incubation. Relative to the ~-galactosidase activity, other glycosidase activities were <-0.03% as determined with p-nitrophenylglycoside substrates [8]. Control mixtures lacked either the ot-galactosidase or the laminin.…”

Section: Terminal A-galactosyl Group Content Of Lamininmentioning

confidence: 86%

“…One piece of the slab was stained in Coomassie blue while the remaining 2 pieces were equilibrated in PBS. One piece was immersed in 20 ml FITC-B4 solution (100 t~g/ml, prepared as in [8]), and the other piece, serving as control, was treated identically except tha t methyl a-D-galactoside (2.5 mg/ml) was added to the lectin solution. After 2 days at 4°C, the gels were rinsed in PBS and photographed under long wavelength ultraviolet illumination (C-62 Blak-Ray Transilluminator, Ultraviolet Products, San Gabriel CA).…”

Section: 'Staining' Sds-polyacrylamide Gels With Fitc-gs I-b4 Isolectinmentioning

confidence: 99%

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Isolation of laminin by affinity chromatography on immobilized Griffonia simplicifolia I lectin

et al. 1982

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Section: Discussionmentioning

confidence: 82%

Section: Terminal A-galactosyl Group Content Of Lamininmentioning

confidence: 86%

Section: 'Staining' Sds-polyacrylamide Gels With Fitc-gs I-b4 Isolectinmentioning

confidence: 99%

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Isolation of laminin by affinity chromatography on immobilized Griffonia simplicifolia I lectin

et al. 1982

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“…The B-subunit shows a high degree of specificity toward α-D-Galp but not α-D-GalNAcp groups. BSL-B4, homo-tetramer of B-subunits, exhibited binding to the glomerular and peritubular basement membrane in frozen sections of mouse and rabbit kidneys, but not in those of humans [28]. Biotinylated BSL-I used in the present study is a mixture of five isolectins, binds mainly with α-D-Galp groups [19].…”

Section: Discussionmentioning

confidence: 99%

Characteristic Changes in Carbohydrate Profile in the Kidneys of Hereditary Nephrotic Mice (ICGN Strain)

Tamura

Manabe

Uchio

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. The ICR-derived glomerulonephritis (ICGN) mice consist of heterozygous and homozygous groups and are considered to be a good model for human idiopathic nephrotic syndrome. To reveal changes in cell-surface carbohydrate construction, 24 lectins were applied to kidney sections of 10-, 30-and 50-week-old male heterozygous and homozygous ICGN mice and age-matched male ICR mice. Bandeiraea simplicifolia lectin-I (BSL-I), which specifically binds to α-D-galactopyranosyl groups, showed positive staining in the glomeruli of ICGN mice, but not in those of ICR mice. Positive BSL-I staining was observed only in distal tubules of homozygous ICGN mice. Lectin blotting for BSL-I demonstrated characteristic glycoproteins (45, 58 and 64 kD) in ICGN but not in ICR mice, and the levels of these molecules augmented in homozygous ICGN mice with the progression of renal failure. Moreover, succinylated wheat germ agglutinin, Dolichos biflorus agglutinin, Aleuria aurantia lectin and Ulex europaeus agglutinin-I showed positive staining only in the glomeruli of homozygous ICGN mice, but not in those of heterozygous ICGN or ICR mice. The staining intensities of Ricinus communis agglutinin-I, Phaseolus vulgaris agglutinin-E and -L, Lens culinaris agglutinin and Erythrina cristagalli agglutinin (ECL) in the glomeruli of homozygous ICGN mice were stronger than those of heterozygous ICGN and ICR mice. In conclusion, lectin histochemistry provided useful information for the diagnosis and prognosis of nephrotic lesions. Characteristic BSL-I binding glycoproteins may be pathogenic factors which cause renal disease in ICGN mice and are good tools to investigate the molecular mechanism of renal disorders in ICGN mice. basement membrane and podocytes with extensively fused foot processes were observed [25,26,31]. Such glomerular lesions were also observed in the kidneys of neonatal ICGN mice (4-6 days after birth) [27]. In the occluded renal tubules, thickened basement membranes were also seen. Thus, the ICGN mice showed albuminuria with a rapid shift of the glomerular lesion to glomerulosclerosis during the first five weeks after birth and are considered to be a suitable nephrosis model to investigate the mechanism of human idiopathic nephrotic syndrome. However, the pathological degeneration of the renal cells in ICGN mice has not been determined in detail. Recent studies of lectin histopathology revealed that changes in cell-surface carbohydrates detected by lectin staining are good markers for histopathological diagnosis [18]. Cell-surface carbohydrates play important roles in cell recognition and interactions during cellular differentiation, and changes in cell-surface carbohydrates have been observed in various pathological conditions in both humans and experimental animals. For instance, tumor cells with malignant phenotypes show neoplastic transformation-related changes in oligosaccharide biosynthesis [2]. Cell-surface carbohydrates are reliable markers for histopathological diagnosis, and lectins recognizing these macromolecules...

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“…GS-I lectin, or its B 4 isomer, binds selectively to basement membranes of microvessels, 12 allowing for excellent visualization of the microvasculature.…”

Section: Microvessel Changes In Hypertension Measured By Griffonia Simentioning

confidence: 99%

Microvessel changes in hypertension measured by Griffonia simplicifolia I lectin.

et al. 1990

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Commonly used methods for assessing reductions in microvascular density (rarefaction) in hypertension detect only perfused microvessels. In the present study, samples of cremaster and spinotrapezius muscles were taken from rats with chronic (4-week) reduced renal mass hypertension and normotensive sham-operated control rats, as well as from 12-week-old spontaneously hypertensive rats and their normotensive Wistar-Kyoto control strain. Mean arterial pressure was 149±8 mm Hg in the rats with reduced renal mass hypertension, 114±7 mm Hg in sham-operated rats, 177±9 mm Hg in spontaneously hypertensive rats, and 95±4 mm Hg in Wistar-Kyoto rats. Muscle samples were incubated with rhodamine-labeled Griffonia simplicifolia I lectin, which identifies both perfused and nonperfused microvessels. Microvascular density was assessed by counting intersections with a 20-/um grid. Microvessel density was significantly reduced in cremaster muscles of both spontaneously hypertensive and reduced renal mass hypertensive rats, and in the spinotrapezius muscle of spontaneously hypertensive rats, compared with their respective normotensive controls. Further studies in the reduced renal mass rats on low salt diets indicated that lectin binding was also decreased as salt intake was increased, independent of blood pressure. This change was not due to an alteration in lectin-binding affinity. These studies indicate that lectin binding can be a useful tool for assessing microvessel density that does not depend on the perfusion state of the vessels and that rarefaction due to hypertension is not evenly distributed in all vascular beds. These results also provide evidence that dietary salt intake alone can influence microvessel density, as measured by the lectin technique. {Hypertension 1990;15:779-783) C onsiderable evidence has been accumulated to indicate that both changes in vessel caliber and vessel number can contribute to the increased vascular resistance in hypertension.

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The use of fluorescein-conjugated Bandeiraea simplicifolia B4-isolectin as a histochemical reagent for the detection of α-d-galactopyranosyl groups

Cited by 185 publications

References 35 publications

Isolation of laminin by affinity chromatography on immobilized Griffonia simplicifolia I lectin

Isolation of laminin by affinity chromatography on immobilized Griffonia simplicifolia I lectin

Characteristic Changes in Carbohydrate Profile in the Kidneys of Hereditary Nephrotic Mice (ICGN Strain)

Microvessel changes in hypertension measured by Griffonia simplicifolia I lectin.

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