The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

Jolley, Michael E.; Nasir, Muh.

doi:10.2174/138620703106298419

Cited by 19 publications

(8 citation statements)

References 32 publications

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“…Rotational speed is increased if the size of the particle increases, for example through specific binding of antibody to the antigen. Consequently, this technique can be readily adapted to the detection of either antibodies or antigens through the concomitant change in rotational rate (Jolley and Nasir 2003). Ideally, antigens selected for this technique should be small ( c. 20 kDa) to allow maximal change mediated through interaction with antibodies.…”

Section: Diagnosticsmentioning

confidence: 99%

Brucellosis - new aspects of an old disease

Cutler¹,

Whatmore²,

Commander³

2005

Journal of Applied Microbiology

195

134

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Section: Diagnosticsmentioning

confidence: 99%

Brucellosis - new aspects of an old disease

Cutler¹,

Whatmore²,

Commander³

2005

Journal of Applied Microbiology

195

134

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“…Serological tests detecting humoral immune responses may be more useful to detect late stage diseased animals. Fluorescence polarization assays (FPA) constitute a technique for antibody detection with a shown potential for diagnostic purposes [20]. An assay for the detection of M. bovis antibodies has been described recently [21]–[25].…”

Section: Introductionmentioning

confidence: 99%

Bayesian Receiver Operating Characteristic Estimation of Multiple Tests for Diagnosis of Bovine Tuberculosis in Chadian Cattle

et al. 2009

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BackgroundBovine tuberculosis (BTB) today primarily affects developing countries. In Africa, the disease is present essentially on the whole continent; however, little accurate information on its distribution and prevalence is available. Also, attempts to evaluate diagnostic tests for BTB in naturally infected cattle are scarce and mostly complicated by the absence of knowledge of the true disease status of the tested animals. However, diagnostic test evaluation in a given setting is a prerequisite for the implementation of local surveillance schemes and control measures.Methodology/Principal FindingsWe subjected a slaughterhouse population of 954 Chadian cattle to single intra-dermal comparative cervical tuberculin (SICCT) testing and two recently developed fluorescence polarization assays (FPA). Using a Bayesian modeling approach we computed the receiver operating characteristic (ROC) curve of each diagnostic test, the true disease prevalence in the sampled population and the disease status of all sampled animals in the absence of knowledge of the true disease status of the sampled animals. In our Chadian setting, SICCT performed better if the cut-off for positive test interpretation was lowered from >4 mm (OIE standard cut-off) to >2 mm. Using this cut-off, SICCT showed a sensitivity and specificity of 66% and 89%, respectively. Both FPA tests showed sensitivities below 50% but specificities above 90%. The true disease prevalence was estimated at 8%. Altogether, 11% of the sampled animals showed gross visible tuberculous lesions. However, modeling of the BTB disease status of the sampled animals indicated that 72% of the suspected tuberculosis lesions detected during standard meat inspections were due to other pathogens than Mycobacterium bovis.Conclusions/SignificanceOur results have important implications for BTB diagnosis in a high incidence sub-Saharan African setting and demonstrate the practicability of our Bayesian approach for diagnostic test evaluation.

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“…The principles of FPIA have been reviewed (Gutierrez et al. , 1989; Eremin & Smith, 2003; Jolley & Nasir, 2003).…”

Section: Introductionmentioning

confidence: 99%

Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay

Wang

Zhang

Murtazina

et al. 2007

Int J of Food Sci Tech

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A fluorescence polarisation immunoassay (FPIA) using a specific polyclonal antiserum for the detection of maduramicin (MD) was developed and optimised. The polyclonal antiserum was produced against MD linked to bovine serum albumin. Fluorescein-labelled MD (tracer) was synthesised by N-hydroxysuccinimide active ester method and purified using thin layer chromatography. The developed FPIA for MD had a dynamic range from 0.01 to 5.6 lg mL )1 with an IC 50 value of 0.16 lg mL )1 and a limit of detection of 0.002 lg mL )1 . Recoveries from chicken muscle, fat and egg samples spiked at 0.25, 5 and 10 lg g )1 levels were 82-130%. The FPIA results from analysis of incurred residues in chicken muscle samples showed that the simple procedure is viable.

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The Use of Fluorescence Polarization Assays for the Detection of Infectious Diseases

Cited by 19 publications

References 32 publications

Brucellosis - new aspects of an old disease

Brucellosis - new aspects of an old disease

Bayesian Receiver Operating Characteristic Estimation of Multiple Tests for Diagnosis of Bovine Tuberculosis in Chadian Cattle

Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay

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