1985
DOI: 10.1111/j.1699-0463.1985.tb02920.x
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The Use of Frozen Monocytes in Phagocytosis Studies

Abstract: Human monocytes were isolated from peripheral blood by Lymphoprep density‐gradient centrifugation and adherence to fibronectin. The cells were loosened by ethylene‐diamino‐tetra‐acetate (EDTA), frozen by different freezing methods, thawed, washed and compared to unfrozen cells. After freezing, thawing and washing, cell recovery was calculated and found to vary with the freezing procedure. The best result was about 76% cell recovery. No morphological differences were observed between unfrozen and frozen cells. … Show more

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