Background: Kahoot is a web-based learning that uses worldwide in the education field. From the review literature, lecture with a Kahoot web-based learning will improve the learning skill. However, there have been a few studies of using Kahoot web-based learning in pharmacy education. Therefore, the aim of this study is to compare pre-and post -learning skill using Kahoot web-based learning and observe the trend of effect for 2 generations. Method: Pre-and Posttest was established in Clinical Pharmacokinetics class of 5 th year Pharmacy Students for 2 consecutive generations. Before class, the students did the questionnaire to evaluate learning skill and then this group was beginning to enroll the class using Kahoot web-based learning. The 8 dimensions of learning skills; Literacy, Numeracy, Reasoning, Creative problem solving, Critical thinking, Collaborative, Communication and Computing were evaluated. The trend of effect was observed. Results: The data from 21 Pharmacy Students (11 from year 2016, 10 from year 2017) who enroll Clinical Pharmacokinetics course in topic "Therapeutic Drug Monitoring of Aminoglycosides" shown that improved of 8 dimension skills scores after learning with Kahoot web-based as follow (Mean ± SD); Literacy (3.29 ± 0.64 vs 4.19 ± 0.40), Numeracy (3.24 ± 0.83 vs 4.05 ± 0.50), Reasoning (3.29 ± 0.56 vs 4.19 ± 0.40), Creative problem thinking (3.05 ± 0.59 vs 4.19+ 0.60), Critical thinking (3.10 ± 0.70 vs 4.10 ± 0.62), Collaborative (3.19 ± 0.75 vs 4.19 ± 0.40), Communication (3.24 ± 0.62 vs 4.24 ± 0.44) and Computing (3.14 ± 0.73 vs 4.00 ± 0.62). The separate analysis results had the same trends. Conclusion: From the results, Kahoot webbased leaning could improve learning skill in Pharmacy Students. The effects consist in 2 generations. However, the confirmation study in the large group also needed.