The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence

Aurílio, Rafaela Baroni; Marsili, Vivian Vidal; Malaquias, Thiago da Silva Santos; Kritski, Afrânio Lineu; Sant’Anna, Clemax Couto

doi:10.1590/0037-8682-0104-2020

Cited by 5 publications

(5 citation statements)

References 9 publications

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“…Ultra detected 67% (4/6) of the patients with enlarged lymph node TB. This percentage is a little higher than in a previous study (56%; 5/9) carried out in our hospital, although the lymph node TB samples were small in both studies 5 . This finding corroborates the Ultra’s superiority over Xpert in the diagnosis of paucibacillary forms of TB.…”

Section: Resultscontrasting

confidence: 71%

Gene-Xpert Ultra for the diagnosis of extrapulmonary tuberculosis in children and adolescents

Aurílio

Ferreira

Parente

et al. 2022

Rev. Inst. Med. trop. S. Paulo

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This prospective study describes the use of Gene-Xpert Ultra for the diagnosis of extrapulmonary tuberculosis (EPTB) in children and adolescents, in Rio de Janeiro, Brazil. Eighteen patients were studied; the final diagnosis of EPTB was established in 13 (72%). Gene-Xpert Ultra results showed detection in 10/13 (77%) of EPTB cases (7 of these 10 with trace-positive results). Gene-Xpert Ultra proved to be a promising method for the diagnosis of childhood EPTB.

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Section: Resultscontrasting

confidence: 71%

Gene-Xpert Ultra for the diagnosis of extrapulmonary tuberculosis in children and adolescents

Aurílio

Ferreira

Parente

et al. 2022

Rev. Inst. Med. trop. S. Paulo

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“…Clinical studies have shown that when patients developed tuberculous meningitis, localized hematoma fluid appeared, which led to an abnormal rise in inflammatory-related factors. Inflammatory factors and hematoma secretions can lead to a rapid increase in Gene Xpert MTB/RIF (13,14). In this study, Gene Xpert MTB/ RIF was found to be highly expressed in tuberculous meningitis, and the level of Gene Xpert MTB/RIF increased with the amount of hemorrhage, indicating that Gene Xpert MTB/RIF is closely related to tuberculous meningitis.…”

Section: Comparison Of Ada and Tb-dna In Non-tuberculous Meningitis G...mentioning

confidence: 48%

The value of gene Xpert MTB / RIF, ADA, TB-DNA in the early diagnosis of TB meningitis

Han Yang,

Aifang Li,

Yongfeng Zhang

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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This study was to explore the expression and correlation between gene Xpert MTB / RIF, ADA, and TB-DNA in TB meningitis. For this purpose, we selected 102 patients in the TB meningitis progression diagnosed and treated in our hospital from January 2019 to December 2020, and another 100 patients in the non-TB meningitis group were selected for the control experiment. Two sets of CSF samples were taken to analyze the gene Xpert MTB / RIF positive rate and the correlation between the expression and the progression of tuberculous meningitis by testing the levels of ADA and TB-DNA in the patient body using an automatic biochemical analyzer. Research indicated that The levels of gene Xpert MTB / RIF, ADA, and TB-DNA in the non-tuberculous meningitis group were lower than those in the tuberculous meningitis group (P<0.05; Levels of gene Xpert MTB / RIF, ADA, and TB-DNA were higher (P<0.05) in patients with group III tuberculous meningitis compared with those under grades I-II tuberculous meningitis, and levels of gene Xpert MTB / RIF, ADA, and TB-DNA were higher (P<0.05) in patients with group VI tuberculous meningitis compared with group III tuberculous meningitis; Gene Xpert MTB / RIF, ADA, TB-DNA) as factors occurring in TB meningitis progression, and all three were associated (P<0.05) with TB meningitis progression; Gene Xpert MTB / RIF, ADA showed a positive correlation (r = 0.296, P = 0.002); Gene Xpert MTB / RIF, TB-DNA showed a positive correlation (r = 0.422, P = 0.001); ADA, TB-DNA showed a positive correlation (r = 0.366, P = 0.001). It was concluded that Gene X-Pert MTB / RIF, ADA, and TB-DNA showed high levels in TB meningitis progression, and as the disease worsened, all three showed a positive association in TB meningitis progression.

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“…After reviewing the titles and abstracts, 31 articles in total were entered into the full-text screening process. After eliminating 10 articles with noncompliant study populations, 1 article with noncompliant disease types and 12 articles with insufficient data, 8 articles [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ] were finally included for full-text review and meta-analysis. The flow of the screening is shown in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

“…(1) In terms of sample size, Alexander W. Kay included 318 samples, and Young Seok Seo included 277 samples, for a total of 527 samples being considered in the present study. After comparing the publication dates of the included studies, we found that a total of four newly published articles (Mijovic [ 26 ], Gautam [ 27 ], Aurilio [ 28 ], Promsena [ 29 ]) were included in this study, adding 369 samples to the analysis. The larger sample size means that the assessment of the diagnostic accuracy of Xpert is more convincing, and the inclusion of newly published articles is more indicative of the diagnostic accuracy of Xpert over recent time periods.…”

Section: Discussionmentioning

confidence: 99%

Xpert MTB/RIF Assay for the Diagnosis of Lymph Node Tuberculosis in Children: A Systematic Review and Meta-Analysis

Chen

Liu

Wang

et al. 2022

JCM

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Background: Lymph node tuberculosis (LNTB) is the leading type of extrapulmonary tuberculosis (EPTB) causing death in children. The Xpert MTB/RIF assay is a novel rapid test for the diagnosis of LNTB. Although previous evidence suggests that Xpert is reliably accurate in diagnosing EPTB in children, information is lacking for the specific type of LNTB in children. The aim of this study was to systematically assess the accuracy and reliability of Xpert for the diagnosis of LNTB in children. Methods: We systematically searched four databases, Embase, Cochrane Library, PubMed, and Web of Science, which extracted relevant data according to predefined inclusion and exclusion criteria. The data were analyzed by meta-Disc 1.4 and Stata 12.0 software to determine sensitivity, specificity, diagnostic odds ratio (DOR), etc. Results: A total of 646 samples from 8 studies were included in the analysis. The pooled sensitivity, specificity, negative likelihood ratio (NLR), positive likelihood ratio (PLR,) and combined diagnostic odds ratio (DOR) of Xpert for all samples were 0.79 (95% CI 0.70, 0.87), 0.90 (95% CI 0.86, 0.92), 0.29 (95% CI 0.19, 0.43), 7.20 (95% CI 3.32, 15.60), and 37.56 (95% CI 13.04, 108.15), respectively. The area under the curve (AUC) of the summary receiver operating characteristic (sROC) curve was 0.9050. Conclusion: Overall, Xpert showed moderate sensitivity and high specificity compared with culture in the diagnosis of LNTB in children. In addition, after analyzing the combined diagnostic odds ratio and positive LR, our study showed that Xpert has excellent diagnostic accuracy.

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The use of Gene-Xpert MTB RIF in the diagnosis of extrapulmonary tuberculosis in childhood and adolescence

Cited by 5 publications

References 9 publications

Gene-Xpert Ultra for the diagnosis of extrapulmonary tuberculosis in children and adolescents

Gene-Xpert Ultra for the diagnosis of extrapulmonary tuberculosis in children and adolescents

The value of gene Xpert MTB / RIF, ADA, TB-DNA in the early diagnosis of TB meningitis

Xpert MTB/RIF Assay for the Diagnosis of Lymph Node Tuberculosis in Children: A Systematic Review and Meta-Analysis

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