The Use of Hemolysin Inhibition in the Study of Experimental And Clinical Hyperlipemia: THE NON-SPECIFIC INHIBITION OF STREPTOLYSIN O BY SERUM LIPOPROTEINS1

Stollerman, Gene H.

doi:10.1172/jci102998

Cited by 13 publications

(6 citation statements)

References 19 publications

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“…It is suggestive that this represents a cholesterol serum fraction which is neither esterified nor combined to lipoproteins, possibly because of saturation of binding sites on the latter. These findings are similar to those of Stollerman (1954) who also showed that addition of heparin to serum from cholesterol-fed rabbits increased the antistreptolysin 0 effect. Horse serum appears less capable of binding added cholesterol since increased antistreptolysin 0 activity was noted when the concentration was increased from the base level of 1200 ,ug/ml to 1800 ,ug/ml a rise of only 50%.…”

Section: Discussionsupporting

confidence: 90%

“…Lecithinase derived from Cl. welchii has a similar effect (Stollerman, 1954). Conversely, removal of non-esterified cholesterol with digitonin before treatment with pancreatin did not increase antistreptolysin 0 activity of the serum since the cholesterol freed by splitting the ester linkage is presumably able to attach to available lipoprotein sites.…”

Section: Discussionmentioning

confidence: 91%

“…However it is known that high antistreptolysin values may be found in cholestatic liver disease (Badin, Cabau, Levy, and Cachin, 1962) and nephrosis (Stollerman, 1954). Badin and Barillec (1970) have shown that these may be distinguished from true antibody-mediated antistreptolysin activity since digitonin will remove that activity in the former case but has no effect on antistreptolysin levels due to immunoglobulins.…”

Section: Discussionmentioning

confidence: 99%

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Some factors influencing the effect of cholesterol on streptolysin O activity

1972

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SYNOPSIS Inhibition of streptolysin 0 activity by cholesterol depends on the latter being in free form. The normal esterified and protein-bound cholesterol fractions in serum do not influence streptolysin 0 activity. However, high cholesterol levels in rabbits fed cholesterol with cholic acid were associated with an increased antistreptolysin 0 effect. It is suggested that this occurs when all available protein sites are saturated and where a true 'free' cholesterol fraction is present.Splitting the esterified cholesterol fraction of human sera with raised cholesterol levels, by means of pancreatin, produced an increased antistreptolysin 0 effect, again presumably because of saturation of protein-binding sites. Similarly, removal of non-esterified cholesterol from sera of cholesterolfed rabbits, by means of digitonin, reduced antistreptolysin 0 activity of the sera.Evidence is presented that combination of bovine serum albumin and streptolysin 0 has a steric hindrance effect on attachment of cholesterol to streptolysin 0.The method described for the estimation of free cholesterol is extremely sensitive, being capable of detecting concentrations of less than 1 0 ,ug/ml.Confirmation of group A streptococcal infection by estimation of antistreptolysin activity remains one of the most useful of serological tests in terms of reproducibility, clarity of end-point, high percentage of positive results, and relative absence of nonspecific reactions. Non-specific positive reactions are known to be obtained with sera contaminated with bacteria. Strains of the genus Pseudomonas appear to be especially involved (Watson, Rose, and Kerr, 1971). Additionally it is known that cholesterol can inhibit reduced streptolysin 'O' (SLO) activity (Hewitt and Todd, 1939). The mechanism of the latter has been studied by Alouf and Raynaud (1968), who have shown that cholesterol acts by combining with the reduced SLO and prevents its attachment to cell membranes. Haemolysis by saponins is also inhibited by cholesterol but the mechanism of action appears different.The present investigation was undertaken to determine the effect of variations in serum cholesterol levels on streptolysin 0 activity and also some of the factors influencing cholesterol inhibition of streptolysin 0.Received for publication 17 August 1972. Materials and Methods ANTISTREPTOLYSIN ESTIMATIONSThese were done by the standard method of Rantz and Randall (1945). Briefly, heat inactivated serum (560 for 30 minutes) is diluted in phosphate-buffered saline (pH 6 7) from three master dilutions (1/10, 1/100, and 1/500). To each 1I0 ml volume of serum dilution in 3 in. x i in. tubes is added 0 5 ml of reduced streptolysin 0 (containing 1.0 IU

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Some factors influencing the effect of cholesterol on streptolysin O activity

1972

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“…However, a second mechanism involves inhibition of streptolysin by cholesterol. The latter has been recorded in patients with jaundice (Badin et al, 1962), in nephrosis (Stollerman, 1954), and in patients with staphylococcal infections (Watson and Kerr, 1975). The latex particle test has been thought to give positive results only with antibody but we have shown that false-positive results can arise (Watson et al, 1977).…”

mentioning

confidence: 69%

Polyene antibiotics in assessing significance of antistreptolysin O activity.

Watson¹,

Kerr²

1978

J Clin Pathol

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“…A marked characteristic is high antistreptolysin-O titer (ASO titer) due to infection of the upper respiratory tract occurring from I to a few weeks before the onset oflesions (1)(2)(3)(4)(5). Regarding ASO titer, however, it has recently been found that an apparent increase may be caused not only by an increase of antistreptolysin-O antibody but also by a non-specific binding of serum low-density lipoproteins with streptolysin-O (6,7). In this work, the specificity of high ASO titer in AGP was examined, as we had found an increase of serum~-lipoproteins in the eruptive stage of this disease.…”

mentioning

confidence: 99%

Unspecific Increase of Antistreptolysin‐o Titer in Acute Guttate Psoriasis

Sato

Ishikawa

1977

The Journal of Dermatology

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An acute streptococcal infection is well-known to be a predisposing factor in acute guttate psoriasis. This study also revealed a high ASO titer in 11 of 13 patients at first examination. However, a non-specific ASO increase due to non-immunological reaction of serum lipoproteins with antistreptolysin-O, was found in 5 of these patients who visited our clinic at the evolving phase, 2 or 3 weeks after onset of the eruption but was no longer observed at 5-6 weeks and thereafter. On the other hand, specific ASO increase, which was not related to lipoproteins, was observed first in some patients at 3-4 weeks and at 7-10 weeks in all patients examined. These observations suggest the role of streptococcal lipoprotein alteration in the occurrence of this disease.

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The Use of Hemolysin Inhibition in the Study of Experimental And Clinical Hyperlipemia: THE NON-SPECIFIC INHIBITION OF STREPTOLYSIN O BY SERUM LIPOPROTEINS1

Cited by 13 publications

References 19 publications

Some factors influencing the effect of cholesterol on streptolysin O activity

Some factors influencing the effect of cholesterol on streptolysin O activity

Polyene antibiotics in assessing significance of antistreptolysin O activity.

Unspecific Increase of Antistreptolysin‐o Titer in Acute Guttate Psoriasis

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