The use of hormone administrations in commercial beef production in the U.K

Kilkenny, J. B.; Sutherland, J. E.

doi:10.1136/vr.87.24.734

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1974

1981

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Cited by 7 publications

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Nachweis von Trenbolon- und Testosteron-R�ckst�nden in Fleisch durch Hochdruckfl�ssigkeitschromatographie

Stan

Hohls

1979

Z Lebensm Unters Forch

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A new method for the qualitative and quantitative determination of trenbolone and testosterone residues in meat, liver, and kidney is described. The analytical procedure consists of the following steps: homogenisation of the meat sample with tetrahydrofurane; liquid-liquid partition first between acetonitrile and hexane, then between sodium hydroxide and petroleum ether/benzene; purification of a silica gel column. The purified sample is analysed by high pressure liquid chromatography using silica gel packings. Detection and quantitative determination are performed with a fluorescence and/or a spectrophotometric detector. Fluorescence measurements are carried out applying a self-made silica gel packed flow-cell. The detection limit in meat extracts is 50 ppb for testosterone and 5 ppb for trenbolone.

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