The use of hydrogels in bone-tissue engineering

Park, Jun‐Beom

doi:10.4317/medoral.16.e115

Cited by 51 publications

(40 citation statements)

References 21 publications

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“…Angiogenesis in alginate is unlikely since vessels are unable to invade it. Although, alginate and other hydrogels have been used for research on bone regeneration (Park, 2011), modification of hydrogels with bioactive molecules (e.g. RGDs) seemed important allowing cell attachment and thereby vessel ingrowth.…”

Section: Discussion With Reviewersmentioning

confidence: 99%

The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage

Pleumeekers

Nimeskern²,

Koevoet³

et al. 2014

eCM

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Cartilage has limited self-regenerative capacity. Tissue engineering can offer promising solutions for reconstruction of missing or damaged cartilage. A major challenge herein is to define an appropriate cell source that is capable of generating a stable and functional matrix. This study evaluated the performance of culture-expanded human chondrocytes from ear (EC), nose (NC) and articular joint (AC), as well as bone-marrow-derived and adipose-tissuederived mesenchymal stem cells both in vitro and in vivo. All cells (≥ 3 donors per source) were culture-expanded, encapsulated in alginate and cultured for 5 weeks. Subsequently, constructs were implanted subcutaneously for 8 additional weeks. Before and after implantation, glycosaminoglycan (GAG) and collagen content were measured using biochemical assays. Mechanical properties were determined using stress-strain-indentation tests. Hypertrophic differentiation was evaluated with qRT-PCR and subsequent endochondral ossification with histology. ACs had higher chondrogenic potential in vitro than the other cell sources, as assessed by gene expression and GAG content (p < 0.001). However, after implantation, ACs did not further increase their matrix. In contrast, ECs and NCs continued producing matrix in vivo leading to higher GAG content (p < 0.001) and elastic modulus. For NC-constructs, matrix-deposition was associated with the elastic modulus (R 2 = 0.477, p = 0.039). Although all cells -except ACsexpressed markers for hypertrophic differentiation in vitro, there was no bone formed in vivo. Our work shows that cartilage formation and functionality depends on the cell source used. ACs possess the highest chondrogenic capacity in vitro, while ECs and NCs are most potent in vivo, making them attractive cell sources for cartilage repair.

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Section: Discussion With Reviewersmentioning

confidence: 99%

The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage

Pleumeekers

Nimeskern²,

Koevoet³

et al. 2014

eCM

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“…Various hybrid materials combined as co-polymers, polymer blends and polymer-ceramic blends have also shown efficacy [67][68][69][70][71][72][73]. Advanced hydrogels, naturally derived collagen and gelatin gels as well as synthetic polyethylene glycol and poly-vinyl alcohol-based hydrogels, serve as matrices for other products and mimic the extracellular matrix topography [74][75][76]. Biomaterials with immunomodulatory strategies, such as artificial extracellular matrices (ECMs) (hydrogels, ECM coatings) and materials with surface property modulation, have the ability to modify the immune function and improve bone repair and regeneration [25,77].…”

Section: Biomaterialsmentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

130

116

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“…[1][2][3][4] Hydrogels have been mainly considered for soft tissue regeneration, 5 but recent advances have allowed for the creation of multifunctional biomaterials with applications to hard tissue engineering. 6 For bone tissue engineering, hydrogel scaffolds are able to present the seeded cells with mechanical, physical, and chemical cues similar in nature to those found in the early stages of fracture healing. 7 Thus, hydrogels can recreate the complex interaction between cells and their microenvironments to regulate tissue morphogenesis and function.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Pandit

Zuidema

Venuto

et al. 2013

Tissue Engineering Part A

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The use of hydrogels for bone regeneration has been limited due to their inherent low modulus to support cell adhesion and proliferation as well as their susceptibility to bacterial infections at the wound site. To overcome these limitations, we evaluated multifunctional polysaccharide hydrogels of varying stiffness to obtain the optimum stiffness at which the gels (1) induce proliferation of human dermal fibroblasts, human umbilical vascular endothelial cells (HUVECs), and murine preosteoblasts (MC3T3-E1), (2) induce osteoblast differentiation and mineralization, and (3) exhibit an antibacterial activity. Rheological studies demonstrated that the stiffness of hydrogels made of a polysaccharide blend of methylcellulose, chitosan, and agarose was increased by crosslinking the chitosan component to different extents with increasing amounts of genipin. The gelation time decreased (from 210 to 60 min) with increasing genipin concentrations. Proliferation of HUVECs decreased by 10.7 times with increasing gel stiffness, in contrast to fibroblasts and osteoblasts, where it increased with gel stiffness by 6.37 and 7.8 times, respectively. At day 14 up to day 24, osteoblast expression of differentiation markers-osteocalcin, osteopontin-and early mineralization marker-alkaline phosphatase, were significantly enhanced in the 0.5% (w/v) crosslinked gel, which also demonstrated enhanced mineralization by day 25. The antibacterial efficacy of the hydrogels decreased with the increasing degree of crosslinking as demonstrated by biofilm formation experiments, but gels crosslinked with 0.5% (w/v) genipin still demonstrated significant bacterial inhibition. Based on these results, gels crosslinked with 0.5% (w/v) genipin, where 33% of available groups on chitosan were crosslinked, exhibited a stiffness of 502 -64.5 Pa and demonstrated the optimal characteristics to support bone regeneration.

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The use of hydrogels in bone-tissue engineering

Cited by 51 publications

References 21 publications

The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage

The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage

The rational use of animal models in the evaluation of novel bone regenerative therapies

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

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