The use of hydrolysates of plant raw materials in the biosynthesis of bacterial cellulose

Abstract

One of the directions for improving the technical and economic indicators of biotechnological production of bacterial cellulose is the use of nutrient media based on modified plant raw materials. Vegetable hydrolysates were obtained by biotransformation of wheat bran with a complex of amylolytic enzyme preparations: Glucolux A, Amylolux ATS, CelloLux A. The total content of mono- and disaccharides in hydrolysate 2.7% and nitrogenous compounds 0.34 g/ l was determined. Bacterial cellulose (BC) biosynthesis using a symbiotic culture of Medusomyces gisevii was carried out under static conditions on the obtained wheat hydrolysates. BC production was 2.71±0.32 g/l on the 21st day of biosynthesis. The structure of the gel film synthesized by BC is characterized by long fibrils up to 10 microns long with a pronounced nanorelief, which are intertwined at an acute angle. Nodes are observed along the fibrils, the distance between which is about 0.5 microns.