Evaluation of a new‐to‐science Artemia population and comparison with four other existing populations were carried out. Five parthenogenetic Artemia populations from the following Greek saltworks were studied: Alyki (a new population), Kalloni, Milos, Polychnitos and Messolongi. The diameters of non‐decapsulated and decapsulated cysts as well as the lengths of nauplii instar stages I, II and III were measured. In addition, the fatty acid composition of decapsulated cysts was estimated. The cyst size of the Alyki population was the smallest, with the largest chorion found in Greece so far. The Messolongi population had the largest cysts, with the smallest chorion of all five populations. The diameter of the non‐decapsulated cysts was highly related (P<0.01) to the length of the nauplii instar I, II and III. The diameter of decapsulated cysts was found to be the most stable and reliable biometric characteristic. The study of their fatty acids profiles revealed that the Polychnitos and Messolongi populations are suitable as live food for freshwater species, while the Alyki, Milos and Kalloni populations are suitable for marine species, having high levels (8.9%, 11.2% and 12.5% respectively) of eicosapentaenoic acid and detectable amounts (0.3%, 0.1% and 0.2% respectively) of docosahexaenoic acid.