The use of <i>Hericium erinaceus</i> and <i>Trametes versicolor</i> extracts in supportive treatment in oncology

Winder, Mateusz; Bulska-Będkowska, Weronika; Chudek, Jerzy

doi:10.2478/acph-2021-0007

Cited by 6 publications

(1 citation statement)

References 53 publications

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“…White rot fungus Trametes versicolor is a biotechnologically important fungus. It is also a medicinal fungus (Kılıç & Yeşilada, 2013;Winder et al, 2021). T. versicolor was also the effective producer of the laccase enzyme.…”

Section: Resultsmentioning

confidence: 99%

Yeni İzole Edilen Trametes versicolor'un Kesikli, Tekrarlı-Kesikli ve Katı-Faz Fermentasyon Süreçlerinde Lakkaz Üretimi

2022

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In this study, the laccase production ability of the newly isolated Trametes versicolor strain was investigated in three different fermentation processes. In all three fermentation processes, the fungus was able to produce the laccase enzyme. During the solid-state fermentation process 13.21 U/mL laccase activity was detected on the 20th day in the 10 mM copper-containing medium, while this value reached to 27.30 U/mL in the medium containing 0.5 mM ABTS+10 mM copper. During the liquid batch fermentation process, laccase activity was significantly induced in the medium containing 1 mM copper and the laccase activities reached 2.25, 19.83 and 24.57 U/mL compared to the medium without copper on the 3rd, 6th, and 9th days, respectively. ABTS and xylidine induced the laccase production of this strain at a much lower level than copper. The liquid repeated-batch process also significantly induced the laccase production. While low level of enzyme activities were detected in a copper-free medium, laccase activities were induced in the copper-containing medium and the activity increased from 0.66 U/mL to 9.87 U/mL at the 6th use of the pellets. Copper was detected as an effective inducer for laccase production in all fermentation processes and activity staining after native polyacrylamide gel electrophoresis clearly showed the active laccase bands. The results revealed that this strain is a good laccase producer and the laccase production yield varies depending on the fermentation process, production time, and inducer used.

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Section: Resultsmentioning

confidence: 99%