The use of<i>Listeria monocytogenes</i>as a DNA delivery vector for cancer gene therapy

Tangney, Mark; Pijkeren, Jan Peter van; Gahan, Cormac G. M.

doi:10.4161/bbug.1.4.11725

Cited by 20 publications

(16 citation statements)

References 15 publications

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“…To date, the genera of bacteria that have been exploited as gene delivery vehicles include Salmonella, Escherichia, Listeria, Clostridium and Bifidobacterium. [9][10][11][12][13][14][15][16][17] These bacteria can be delivered to the tumor via multiple routes such as intra-tumoral 1 injection, intravenous injection or in certain instances orally. Preclinical studies have shown the ability of different bacterial strains to locally produce therapeutic agents and mediate highly effective and specific therapeutic responses.…”

Section: Bacterial-mediated Tumor Therapymentioning

confidence: 99%

Bacterial vectors for imaging and cancer gene therapy: a review

et al. 2012

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The significant burden of resistance to conventional anticancer treatments in patients with advanced disease has prompted the need to explore alternative therapeutic strategies. The challenge for oncology researchers is to identify a therapy which is selective for tumors with limited toxicity to normal tissue. Engineered bacteria have the unique potential to overcome traditional therapies' limitations by specifically targeting tumors. It has been shown that bacteria are naturally capable of homing to tumors when systemically administered resulting in high levels of replication locally, either external to (non-invasive species) or within tumor cells (pathogens). Pre-clinical and clinical investigations involving bacterial vectors require relevant means of monitoring vector trafficking and levels over time, and development of bacterial-specific real-time imaging modalities are key for successful development of clinical bacterial gene delivery. This review discusses the currently available imaging technologies and the progress to date exploiting these for monitoring of bacterial gene delivery in vivo.Cancer Gene Therapy ( INTRODUCTIONAlthough the potential anti-cancer effects of acquired bacterial infection have been evident for over 120 years and the presence of bacteria in excised tumors has been noted for over 60 years, 1 it is only in more recent times that the tumor-specific growth of bacteria has been considered for therapeutic purposes. While the precise mechanism(s) behind preferential bacterial tumor colonization remain poorly understood, this phenomenon must relate to unique tumor attributes that separate them from healthy tissues. Factors such as the irregular blood supply; local immune suppression; inflammation; and unique nutrient supply (e.g., purines) in tumors have been proposed to play a role. 2 Bacterial entry into the tumor environment is proposed to be facilitated by the leaky vasculature. Cancer cells are characterized by an aberrantly accelerated metabolism and proliferation leading to an imbalance of oxygen supply and consumption which is a major causative factor of tumor hypoxia. 3 The hypoxic nature of solid tumors enhances the growth of anaerobic and facultatively anaerobic bacteria. In addition, these areas with low oxygen and high interstitial pressure are considered to be an immunological sanctuary, where bacterial clearance mechanisms are greatly inhibited. 4 Necrotic regions are also rich in nutrients favoured by bacteria due to tumor cell turnover. However, tumor selective bacterial colonization now appears to be both bacterial species and tumor origin independent, since aerobic bacteria are also capable of colonising tumors, and even small tumors lacking an anaerobic center are colonised. See Figure 1 for proposed mechanisms.Invasive bacteria can internalize and replicate within tumor cells, while non-invasive bacteria grow externally to tumor cells, within the tumor micro-environment. 5 The tumor selective growth of bacteria has made them an attractive vehicle for the delivery of ...

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Section: Bacterial-mediated Tumor Therapymentioning

confidence: 99%

Bacterial vectors for imaging and cancer gene therapy: a review

et al. 2012

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“…Tangney et al used an ampicillin-sensitive strain to create a system that could deliver DNA to infected cancer cells [25]. In this system, L. monocytogenes is lysed within the tumor after the administration of ampicillin to release plasmids inside the cells.…”

Section: Delivery Of Dna or Sirnamentioning

confidence: 99%

Salmonella-Mediated Cancer Therapy: Roles and Potential

Nguyen

Min

2016

Nucl Med Mol Imaging

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The use of bacteria for cancer therapy, which was proposed many years ago, was not recognized as a potential therapeutic strategy until recently. Technological advances and updated knowledge have enabled the genetic engineering of bacteria for their safe and effective application in the treatment of cancer. The efficacy of radiotherapy depends mainly on tissue oxygen levels, and low oxygen concentrations in necrotic and hypoxic regions are a common cause of treatment failure. In addition, the distribution of a drug is important for the therapeutic effect of chemotherapy, and the poor vasculature in tumors impairs drug delivery, limiting the efficacy of a drug, especially in necrotic and hypoxic regions. Bacteria-mediated cancer therapy (BMCT) relies on facultative anaerobes that can survive in well or poorly oxygenated regions, and it therefore improves the therapeutic efficacy drug distribution throughout the tumor mass. Since the mid-1990s, the number of published bacterial therapy papers has increased rapidly, with a doubling time of 2.5 years in which the use of increased significantly. BMCT is being reevaluated to overcome some of the drawbacks of conventional therapies. This review focuses on-mediated cancer therapy as the most widely used type of BMCT.2.

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“…Genetically engineered bacteria can serve as tumor-specific vectors (29). Proteins of interest can thus be expressed in a tumor microenvironment enabling powerful therapies to be applied.…”

Section: Microbiology and Cancermentioning

confidence: 99%

Cancer in North Cyprus: 2. Biomedical Research Activities

Djamgoz¹,

Akün²,

Arslan³

et al. 2017

Cyprus J Med Sci

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This is the second part of a two-part review on the cancer status of North Cyprus. Here, we give an overview of the various areas of research on cancer. There are four main areas of ongoing biomedical investigation. First, monitoring of possible carcinogenic chemical factors in the environment (soil and water) is focused on arsenic. Arsenic has been found to exist broadly over the island at levels above the minimum level advised by the Environmental Protection Agency. At present, the source(s) of environmental arsenic is unclear. Second, pathophysiological mechanisms of breast and prostate cancer are being investigated with a focus on ion channels (particularly, the voltage-gated sodium channel) driving the metastatic process. In particular, the triangular relationship involving the channel, its persistent current component, and tumor hypoxia is being elucidated. Third, a further emerging theme is the association of microbiota with cancer. The possible roles of bacterial infections or microbiota in preventing, treating, or causing several types of cancers are among these projects. Fourth, a range of developments in nanotechnology is being considered. It is concluded that the island offers significant opportunities for internationally competitive, multi-faceted research on cancer.

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The use ofListeria monocytogenesas a DNA delivery vector for cancer gene therapy

Cited by 20 publications

References 15 publications

Bacterial vectors for imaging and cancer gene therapy: a review

Bacterial vectors for imaging and cancer gene therapy: a review

Salmonella-Mediated Cancer Therapy: Roles and Potential

Cancer in North Cyprus: 2. Biomedical Research Activities

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