The use of IgE immunoblotting as a diagnostic tool in allergy

García, Milagros; Moneo, Ignacio; Audícana, M.; Pozo, M. D. Del; Muñoz, D.; Fernández, E.; Dı́ez, Jesús; Etxenagusia, M.; Ansotegui, Ignacio J.; Corrés, L. Fernández de

doi:10.1016/s0091-6749(97)70076-9

Cited by 62 publications

(47 citation statements)

References 15 publications

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“…Previous reports indicated that antibodies from fully sensitized patients recognize several different allergens in a crude extract by immunoblotting and immunoelectrophoresis (15,100). This recognition of multiple allergens from a given biological source is now a common finding, such as with grass pollens (272).…”

Section: Anisakis Allergensmentioning

confidence: 94%

“…In the allergic form of anisakidosis, the most relevant immunopathologic finding is the increase in specific and total IgE levels in patients ' sera (4,95,100). Allergic reactions to A. simplex occur within the first few hours after ingestion of infected fish (typically within the first hour).…”

Section: A Simplex Allergy In Humans (I) General Aspects the Potenmentioning

confidence: 99%

“…Immunoblotting studies have shown that human IgE and IgG antibody responses to A. simplex antigens are highly heterogeneous and vary dramatically between individuals, both quantitatively and qualitatively (100,116,147). Diversity in the specificity of the antibody repertoire has also been observed for humans infected with parasites related to A. simplex, and modeling the phenomenon in experimental animals strongly indicates that it is due to genetic differences between individuals, specifically in the class II region of the MHC (145-147, 150, 263).…”

Section: A Simplex Allergy In Humans (I) General Aspects the Potenmentioning

confidence: 99%

“…It was noted in the original work that led to the wide recognition of A. simplex allergy that cooking and freezing cannot be relied upon to destroy allergenicity and protect against hypersensitivity reactions to parasite material contaminating ingested fish (24,(27)(28)(29)100). Recently, thermostable allergens have been detected in A. simplex extracts (reviewed in the previous section), and this recalls the controversy about the safety of human consumption of these parasites in cooked fish (48,188).…”

Section: Anisakis Allergensmentioning

confidence: 99%

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Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

2008

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SUMMARY Infection of humans with the nematode worm parasite Anisakis simplex was first described in the 1960s in association with the consumption of raw or undercooked fish. During the 1990s it was realized that even the ingestion of dead worms in food fish can cause severe hypersensitivity reactions, that these may be more prevalent than infection itself, and that this outcome could be associated with food preparations previously considered safe. Not only may allergic symptoms arise from infection by the parasites (“gastroallergic anisakiasis”), but true anaphylactic reactions can also occur following exposure to allergens from dead worms by food-borne, airborne, or skin contact routes. This review discusses A. simplex pathogenesis in humans, covering immune hypersensitivity reactions both in the context of a living infection and in terms of exposure to its allergens by other routes. Over the last 20 years, several studies have concentrated on A. simplex antigen characterization and innate as well as adaptive immune response to this parasite. Molecular characterization of Anisakis allergens and isolation of their encoding cDNAs is now an active field of research that should provide improved diagnostic tools in addition to tools with which to enhance our understanding of pathogenesis and controversial aspects of A. simplex allergy. We also discuss the potential relevance of parasite products such as allergens, proteinases, and proteinase inhibitors and the activation of basophils, eosinophils, and mast cells in the induction of A. simplex-related immune hypersensitivity states induced by exposure to the parasite, dead or alive.

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Section: Anisakis Allergensmentioning

confidence: 94%

Section: A Simplex Allergy In Humans (I) General Aspects the Potenmentioning

confidence: 99%

Section: A Simplex Allergy In Humans (I) General Aspects the Potenmentioning

confidence: 99%

Section: Anisakis Allergensmentioning

confidence: 99%

See 2 more Smart Citations

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

2008

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“…For these reasons, it is probable that only the most severe cases that require invasive techniques are diagnosed and that many of the mild cases remain underdiagnosed (21). Although serologic techniques have been developed for the detection of A. simplex infection, cross-reactivities with other helminths have been a serious limitation (8,10,11,17). In an attempt to improve the diagnosis, we purified and characterized a major immunoglobulin E (IgE)-binding protein from the parasite.…”

mentioning

confidence: 99%

High Prevalence of Seropositivity to a Major Allergen of Anisakis simplex , Ani s 1, in Dyspeptic Patients

Toro

Caballero

Baquero

et al. 2004

Clin Vaccine Immunol

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Finding evidence of anisakidosis requires invasive methods. We have developed a serological assay based on the detection of an immunoglobulin E (IgE) specifically directed against Ani s 1 protein, a major parasite allergen of Anisakis simplex, which has shown a high level of accuracy in the diagnosis of anisakidosis. We used this tool to determine the prevalence of anti-Ani s 1 IgE in dyspeptic patients and to investigate if its seropositivity could be related to epidemiological factors other than raw fish consumption. A total of 174 dyspeptic patients who submitted to upper digestive tract endoscopy were studied. Specific IgE against Ani s 1 was determined by immunoblotting. Quantitative information on smoking, alcohol consumption, and fish consumption as well as a history of gastric surgery was recorded. Twenty-four (13.8%) patients were seropositive for Ani s 1 protein. The seroprevalence of anti-Ani s 1 IgE increased with age in patients who were less than 62 years old (P ‫؍‬ 0.047). Seropositivity to Ani s 1 was associated with the consumption of fish in vinegar (P < 0.001), raw fish (P ‫؍‬ 0.001), and smoked fish (P ‫؍‬ 0.007). There was no relationship between anti-Ani s 1 IgE seropositivity and the number of cigarettes smoked (P ‫؍‬ 0.098) or alcohol intake (P ‫؍‬ 0.179). Five patients had undergone previous gastric surgery, and three of those patients were seropositive for Ani s 1 (P ‫؍‬ 0.019). In multivariate analysis, the consumption of fish in vinegar (P ‫؍‬ 0.006), raw fish (P ‫؍‬ 0.017), and smoked fish (P ‫؍‬ 0.002) and a history of gastric surgery (P ‫؍‬ 0.005) were independent factors associated with anti-Ani s 1 IgE detection. In conclusion, at present, anisakidosis might frequently be underdiagnosed, and it might have a clinical role in patients with upper dyspeptic symptoms. Uncooked-fish ingestion and previous gastric surgery were associated with seropositivity for Ani s 1 protein.

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Occupational airway sensitizers: an overview on the respective literature

2000

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Background Worldwide, there is rigorous scientific activity concerning the further development of work safety regulations involving airway‐sensitizing substances. Technical directives on hazardous substances are enforced in several countries and are being continuously updated. The European Union has established a code for several occupational substances, now labeled R 42 (“may cause sensitization by inhalation”). Methods We present an overview of the literature dealing with allergic occupational asthma. The literature was selected according to criteria of study design and diagnostic test methods. Approximately 300 publications were reviewed including both epidemiological studies and individual case reports. Results Airway sensitizers are systematically arranged and separately listed according to chemicals and their origin from animals, plants, and microorganisms. The clinical data as well as threshold limit values (TLV) and R 42 labeling of 250 airway‐sensitizing substances are presented. Conclusions The most common sensitizing substances causing occupational asthma were dust of cereal flours, enzymes, natural rubber latex, laboratory animals as well as low molecular substances such as isocyanates and acid anhydrides. Am. J. Ind. Med. 38:164–218, 2000. © 2000 Wiley‐Liss, Inc.

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The use of IgE immunoblotting as a diagnostic tool in allergy

Cited by 62 publications

References 15 publications

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

High Prevalence of Seropositivity to a Major Allergen of Anisakis simplex , Ani s 1, in Dyspeptic Patients

Occupational airway sensitizers: an overview on the respective literature

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