The use of immunological methods to detect and identify bacteria in the environment

Schloter, Michael; Aßmus, Bernhard; Hartmann, A

doi:10.1016/0734-9750(94)00023-6

Cited by 76 publications

(39 citation statements)

References 63 publications

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“…Immunotrapping was carried out as described below using aliquots of the suspensions. Immunotrapping was performed as previously described (Biebel et al, 1995 ;Schloter et al, 1995) using mAb 2.11. This antibody showed highest affinity to O. anthropi strains and has surface lipopolysaccharides as antigenic epitopes (M. Schloter, personal communication).…”

Section: Soils and Plants Immunotrapping And Bacterial Strainsmentioning

confidence: 99%

Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.

Lebuhn¹,

Achouak²,

Schloter³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

167

125

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A large collection of bacterial strains, immunotrapped from soil and from the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno-and genotypic parameters. Strains were grouped on (inter) repetitive extragenic palindromic DNA (REP) PCR profiles at the intraspecies level. Pheno-and genotypic characters were assessed for representatives from 13 different REP groups. Strains of nine REP groups constituting two physiological BIOLOG clusters fell in the coherent DNA-DNA reassociation group of Ochrobactrum anthropi. Strains of two REP groups constituting a separate BIOLOG cluster fell in the coherent DNA-DNA reassociation group of Ochrobactrum intermedium. Additional phenotypic characters differentiating O. anthropi and O. intermedium were found. REP group K strains constituted a different BIOLOG cluster, a separate DNA-DNA reassociation group and a distinct phylogenetic lineage in 16S rDNA homology analysis, indicating that REP group K strains represent a new species. Diagnostic phenotypic characters were found. Closest relatives were Ochrobactrum species. The name Ochrobactrum grignonense sp. nov. is proposed (type strain OgA9a T l LMG 18954 T l DSM 13338 T ). REP group J strains again constituted a different BIOLOG cluster, a separate DNA-DNA reassociation group and showed, as a biological particularity, a strict preference for the rhizoplane as habitat. Diagnostic phenotypic characters were found. This indicated that REP group J strains represent a further new species, although phylogenetic analyses using 16S rDNA homology were not able to separate the cluster of REP group J sequences significantly from 16S rDNA sequences of Ochrobactrum anthropi. The name Ochrobactrum tritici sp. nov. is proposed (type strain SCII24 T l LMG 18957 T l DSM 13340 T ).

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Section: Soils and Plants Immunotrapping And Bacterial Strainsmentioning

confidence: 99%

Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.

Lebuhn¹,

Achouak²,

Schloter³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

167

125

View full text Add to dashboard Cite

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“…Entretanto, é importante observar que diluições crescentes de IgG tendem a promover um aumento de especificidade, associado à queda de sensibilidade (Schloter et al, 1995). Tal efeito paradoxal deve ser sempre considerado na definição do título do anti-soro e é fortemente dependente do objetivo da pesquisa.…”

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Produção e caracterização de anticorpos policlonais contra Xanthomonas campestris pv. viticola

Araujo

Reis

Cruz

et al. 2005

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi a produção de anticorpos policlonais contra Xanthomonas campestris pv. viticola e sua caracterização pelo método Elisa indireto. Os resultados apontaram a qualidade dos anticorpos policlonais produzidos, os quais mostraram-se altamente reativos e específicos para o patovar com potencial para ser empregado no diagnóstico da doença e em programas de certificação.Termos para indexação: Vitis vinifera, cancro bacteriano, diagnose. Production and characterization of polyclonal antibodies against Xanthomonas campestris pv. viticolaAbstract -The objective of this work was to produce polyclonal antibodies against Xanthomonas campestris pv. viticola and characterize these antibodies through Elisa serological indirect method. Results indicate that polyclonal antibodies produced were highly reactive against bacterial cells, showing specificity at the pathovar level and potential to be used for diagnosis and certification purposes.

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“…Culture-based methods are time consuming and most importantly, they do not exactly reflect the complexity of microbial diversity (Snaidr et al 1997;Moter & Ulf 2000), thus various molecular techniques including fluorescence in situ hybridization (FISH) (Christensen et al1999;Pernthaler et al 2002), 16S rRNA gene cloning (Arahal et al 1996;Dunbar et al 1999); denaturing gradient gel electrophoresis (DGGE) (Ferris & Ward 1997), flow cytometry (Porter et al 1997) and the immunological method (Schloter et al 1995), have been applied to give more comprehensive and precise characterizations of Bacteria and Archaea in numerous extreme environments.…”

Section: Introductionmentioning

confidence: 99%

Archaeal Community Revealed by 16s rRNA and Fluorescence in situ Hybridization in a Sulphuric Hydrothermal Hot Spring, Northern Taiwan

Chang²,

Shyu

2005

World J Microbiol Biotechnol

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The archaeal community composition of Yangmingshan National Park in northern Taiwan was investigated by 16S rRNA and fluorescence in situ hybridization (FISH). Optimization of tetrameric restriction enzyme (TRE) was performed to achieve efficient digestion and differentiation in the restriction fragment length polymorphism (RFLP) fragments, and AciI, BstUI and RsaI were shown to be the optimal TREs for TRE-RFLP. Nine clones were obtained in the studies, with clones M70 and M6 being found to be phylogenetically affiliated to Sulfolobus and Caldisphaera in domain Crenarchaeota, respectively, whereas seven other clones were found to be affiliated to an uncultured and unidentified archaeon isolated from thermoacidic environments. In FISH, soil and water region cells were hybridized with DAPI (4 0 , 6-diamidino-2-phenylindole) and specific fluorescently labelled probes. 15.69 and 7.16% of the DAPI-stained cells hybridized with universal archaeal probe ARC915 and sulphate-reducing bacterial probe SRB385, respectively.

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The use of immunological methods to detect and identify bacteria in the environment

Cited by 76 publications

References 63 publications

Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.

Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.

Produção e caracterização de anticorpos policlonais contra Xanthomonas campestris pv. viticola

Archaeal Community Revealed by 16s rRNA and Fluorescence in situ Hybridization in a Sulphuric Hydrothermal Hot Spring, Northern Taiwan

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