The Use of Inosine 5′-Monophosphate Dehydrogenase (IMPDH) in the Development of a New Liquid Homogeneous Enzyme Immunoassay Technology

Dorn, Allan R.; Mountain, L; Ghoshal, Mitali; Hui, R.; Klinedinst, Lisa K.; Rugaber, Janice E.; Schamerloh, Andrew F.; Salamone, Salvatore J.

doi:10.1093/clinchem/47.10.1921

Cited by 1 publication

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IMPDH has been associated with cell proliferation and malignancy since 1975 (13). In particular, it has been previously reported that IMPDH2 was the predominant isoform in neoplastic and replicating cells (5). Accumulating evidence has also suggested that IMPDH2 may be closely implicated in different types of malignancy (18)(19)(20)(21).…”

Section: Discussionmentioning

confidence: 99%

“…IMPDH2 was previously reported to affect EMT in colon cancer cells (5), thus the present study investigated whether IMPDH2 may have the same influence on NSCLC cells. Following the overexpression and knockdown of IMPDH2 in A549 cells, the expression levels of the epithelial markers, E-cadherin, and the mesenchymal markers, vimentin and N-cadherin, were analyzed using western blotting.…”

Section: Impdh2 Promotes the Invasion And Migration Of Nsclc Cells Thmentioning

confidence: 94%

“…Inosine 5'-monophosphate dehydrogenase (IMPDH) is a rate-limiting enzyme, which catalyzes the nicotinamide adenine dinucleotide + -dependent oxidation of inosine monophosphate to xanthosine monophosphate during the de novo biosynthesis of guanine nucleotides (4). In fact, IMPDH serves a crucial role in DNA synthesis (5). Human IMPDH exists in two ubiquitously expressed isoforms: IMPDH type I (IMPDH1) and IMPDH type II (IMPDH2), which serve different roles despite the 84% similarity in the amino acid sequence (6).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/β‑catenin signaling pathway

Zha

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

Inosine 5'-monophosphate dehydrogenase type II (IMPDH2) is an important enzyme involved in the biosynthesis of guanine nucleotides. Therefore, the present study aimed to investigate the potential and molecular mechanism of IMPDH2 in non-small cell lung cancer (NSCLC). Reverse transcription-quantitative PCR and immunohistochemistry were used to detect IMPDH2 expression levels in NSCLC tissues and cells. A Cell Counting Kit-8 assay, colony formation assay, flow cytometry, wound healing, Transwell assay, western blotting and immunofluorescence analyses were utilized to identify the effects of upregulated IMPDH2 levels on NSCLC cells. The expression levels of IMPDH2 have been discovered to be upregulated in several types of human cancer; however, the biological and clinical value of IMPDH2 in NSCLC remains unclear. The results of the present study revealed that the expression levels of IMPDH2 were significantly upregulated in NSCLC tissues. Furthermore, the genetic knockdown of IMPDH2 significantly hindered the proliferation, apoptosis, invasion, migration and epithelial-mesenchymal transition of NSCLC cells, whereas the overexpression of IMPDH2 achieved the opposite results. In addition, the results of the present study demonstrated that the inhibition of IMPDH2 inhibited the Wnt/β-catenin signaling pathway by decreasing the expression levels of Wnt3a and β-catenin, while increasing the expression levels of phosphorylated glycogen synthase kinase-3β in NSCLC cells. These findings of the present study indicated that IMPDH2 may promote NSCLC progression by activating the Wnt/β-catenin signaling pathway, which suggested that IMPDH2 may be a novel therapeutic target for patients with NSCLC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Impdh2 Promotes the Invasion And Migration Of Nsclc Cells Thmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/β‑catenin signaling pathway

Zha

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

show abstract

The Use of Inosine 5′-Monophosphate Dehydrogenase (IMPDH) in the Development of a New Liquid Homogeneous Enzyme Immunoassay Technology

Cited by 1 publication

References 3 publications

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/β‑catenin signaling pathway

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/β‑catenin signaling pathway

Contact Info

Product

Resources

About

The Use of Inosine 5′-Monophosphate Dehydrogenase (IMPDH) in the Development of a New Liquid Homogeneous Enzyme Immunoassay Technology

Cited by 1 publication

References 3 publications

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/&beta;‑catenin signaling pathway

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/&beta;‑catenin signaling pathway

Contact Info

Product

Resources

About

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/β‑catenin signaling pathway

IMPDH2 promotes cell proliferation and epithelial‑mesenchymal transition of non‑small cell lung cancer by activating the Wnt/β‑catenin signaling pathway