The use of intravital microscopy in surgical research

Vollmar, Brigitte; Menger, Michael D.

doi:10.1007/s004230050133

Cited by 11 publications

(2 citation statements)

References 25 publications

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“…It is therefore impossible to measure blood perfusion of individual microvessels using these techniques. By contrast, intravital microscopy allows the perfusion of microvessels to be examined over an extended period of time [21,22]. …”

Section: Discussionmentioning

confidence: 99%

Increase in periosteal angiogenesis through heat shock conditioning

et al. 2011

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ObjectiveIt is widely known that stress conditioning can protect microcirculation and induce the release of vasoactive factors for a period of several hours. Little, however, is known about the long-term effects of stress conditioning on microcirculation, especially on the microcirculation of the periosteum of the calvaria. For this reason, we used intravital fluorescence microscopy to investigate the effects of heat shock priming on the microcirculation of the periosteum over a period of several days.MethodsFifty-two Lewis rats were randomized into eight groups. Six groups underwent heat shock priming of the periosteum of the calvaria at 42.5°C, two of them (n = 8) for 15 minutes, two (n = 8) for 25 minutes and two (n = 8) for 35 minutes. After 24 hours, a periosteal chamber was implanted into the heads of the animals of one of each of the two groups mentioned above. Microcirculation and inflammatory responses were studied repeatedly over a period of 14 days using intravital fluorescence microscopy. The expression of heat shock protein (HSP) 70 was examined by immunohistochemistry in three further groups 24 hours after a 15-minute (n = 5), a 25-minute (n = 5) or a 35-minute (n = 5) heat shock treatment. Two groups that did not undergo priming were used as controls. One control group (n = 8) was investigated by intravital microscopy and the other (n = 5) by immunohistochemistry.ResultsDuring the entire observation period of 14 days, the periosteal chambers revealed physiological microcirculation of the periosteum of the calvaria without perfusion failures. A significant (p < 0.05) and continuous increase in functional capillary density was noted from day 5 to day 14 after 25-minute heat shock priming. Whereas a 15-minute exposure did not lead to an increase in functional capillary density, 35-minute priming caused a significant but reversible perfusion failure in capillaries. Non-perfused capillaries in the 35-minute treatment group were reperfused by day 10. Immunohistochemistry demonstrated an increase in cytoprotective HSP70 expression in the periosteum after a 15-minute and a 35-minute heat shock pretreatment when compared with the control group. The level of HSP70 expression that was measured in the periosteum after 25 minutes of treatment was significantly higher than the levels observed after 15 or 35 minutes of heat shock exposure.ConclusionA few days after heat shock priming over an appropriate period of time, a continuous increase in functional capillary density is seen in the periosteum of the calvaria. This increase in perfusion appears to be the result of the induction of angiogenesis.

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Section: Discussionmentioning

confidence: 99%

Increase in periosteal angiogenesis through heat shock conditioning

et al. 2011

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“…In contrast, intravital microscopy is a technique that allows direct visualization of microvascular perfusion, including arteriolar, capillary, and venular segments of the microvasculature. Because intravital microscopy also facilitates the simultaneous study of distinct molecular and cellular functions, the technique may be superior to thermodiffusion, laser Doppler fluxmetry, and microspheres [19].…”

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confidence: 99%