The use of isocyanide-based multicomponent reaction for covalent immobilization of Rhizomucor miehei lipase on multiwall carbon nanotubes and graphene nanosheets

Mohammadi, Mehdi; Ashjari, Maryam; Garmroodi, Maryam; Yousefi, Maryam; Karkhane, Ali Asghar

doi:10.1039/c6ra14142k

Cited by 29 publications

(11 citation statements)

References 38 publications

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“…Therefore, designing novel methods for fixation of enzyme on the supports that can improve the activity of the enzymes in harsh reaction conditions, such as extreme pH, high temperature, or in the presence of organic solvents, is in the spotlight nowadays. Taking advantages of Ugi-4CR for enzyme immobilization on graphene surfaces, in 2016, the Rhizomucor miehei lipase (RML) was immobilized on GO-COOH sheets with the maximum loading capacity of 530 mg and high thermal and cosolvent stability in comparison with the soluble enzyme …”

Section: Covalent Functionalization Through Multicomponent Reactionsmentioning

confidence: 99%

Materials Functionalization with Multicomponent Reactions: State of the Art

Afshari

Shaabani

2018

ACS Comb. Sci.

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The emergence of neoteric synthetic routes for materials functionalization is an interesting phenomenon in materials chemistry. In particular, the union of materials chemistry with multicomponent reactions (MCRs) opens a new avenue leading to the realm of highly innovative functionalized architectures with unique features. MCRs have recently been recognized as considerable part of the synthetic chemist's toolbox due to their great efficiency, inherent molecular diversity, atom and pot economy along with operational simplicity. Also, MCRs can improve E-factor and mass intensity as important green chemistry metrics. By rational tuning of the materials, as well as the MCRs, wide ranges of functionalized materials can be produced with tailorable properties that can play important roles in the plethora of applications. To date, there has not reported any exclusive review of a materials functionalization with MCRs. This critical review highlights the state-of-the-art on the one-pot functionalization of carbonaceous and siliceous materials, polysaccharides, proteins, enzymes, synthetic polymers, etc., via diverse kind of MCRs like Ugi, Passerini, Petasis, Khabachnik-Fields, Biginelli, and MALI reactions through covalent or noncovalent manners. Besides the complementary discussion of synthetic routes, superior properties and detailed applicability of each functionalized material in modern technologies are discussed. Our outlook also emphasizes future strategies for this unprecedented area and their use as materials for industrial implementation. With no doubt, MCRs-functionalization of materials bridges the gap between materials science domain and applied chemistry.

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Section: Covalent Functionalization Through Multicomponent Reactionsmentioning

confidence: 99%

Materials Functionalization with Multicomponent Reactions: State of the Art

Afshari

Shaabani

2018

ACS Comb. Sci.

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“…So far, very few reports about GO-based supports for lipase immobilization have been published. For instance, Jing and co-workers [ 22 ] synthesized a chloropropyl-functionalized graphene oxide decorated with Fe 3 O 4 nanoparticles for the immobilization of porcine pancreatic lipase (PPL); Mohammadi and co-workers [ 23 ] used an isocyanide-based four-component reaction tobimmobilize Rhizomucor miehei lipase (RML) on carboxylated multi-wall carbon nanotubes and carboxylated graphene nanosheets; Li and co-workers [ 24 ] fabricated three dimensional (3D) magnetic graphene oxide-chitosan (GO/CS) composites with orderly self-assembled magnetite (Fe 3 O 4 ) nanoparticles (GO/CS/Fe 3 O 4 ) to was immobilize Candida rugosa lipase (CRL).…”

Section: Introductionmentioning

confidence: 99%

Preparation of Carriers Based on ZnO Nanoparticles Decorated on Graphene Oxide (GO) Nanosheets for Efficient Immobilization of Lipase from Candida rugosa

et al. 2017

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Herein, a promising carrier, graphene oxide (GO) decorated with ZnO nanoparticles, denoted as GO/ZnO composite, has been designed and constructed. This carrier was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetry. Then, Candida rugosa lipase (CRL) was immobilized onto the GO-based materials via physical adsorption. Our results indicated that the lipase loading amount on the GO/ZnO composites was about 73.52 mg of protein per g. In the activity assay, the novel immobilized lipase GO/ZnO@CRL, exhibited particularly excellent performance in terms of thermostability and reusability. Within 30 min at 50 °C, the free lipase, GO@CRL and ZnO@CRL had respectively lost 64%, 62% and 41% of their initial activity. However, GO/ZnO@CRL still retained its activity of 63% after 180 min at 50 °C. After reuse of the GO/ZnO@CRL 14 times, 90% of the initial activity can be recovered. Meanwhile, the relative activity of GO@CRL and ZnO@CRL was 28% and 23% under uniform conditions. Hence, GO-decorated ZnO nanoparticles may possess great potential as carriers for immobilizing lipase in a wide range of applications.

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“…The second mass reduction in GO (21.2%) occurred between 110 to 403°C, which could be associated with the decarboxylation process of the graphene oxide sheets [33] . A mass loss of 34.6% was observed for amine-functionalized GO between 106°C to 411°C due to the removal of chemically added amine groups from the surface.…”

Section: Resultsmentioning

confidence: 93%

The use of isocyanide-based multicomponent reaction for covalent immobilization of Rhizomucor miehei lipase on multiwall carbon nanotubes and graphene nanosheets

Abstract: One-pot immobilization of RML on carbon-based nanomaterials was performed by using the Ugi four component reaction under extremely mild conditions.

Cited by 29 publications

References 38 publications

Materials Functionalization with Multicomponent Reactions: State of the Art

Materials Functionalization with Multicomponent Reactions: State of the Art

Preparation of Carriers Based on ZnO Nanoparticles Decorated on Graphene Oxide (GO) Nanosheets for Efficient Immobilization of Lipase from Candida rugosa

Sortase-mediated immobilization of Candida antarctica lipase B (CalB) on graphene oxide; comparison with chemical approach

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