THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

Reynolds, Edward S.

doi:10.1083/jcb.17.1.208

Cited by 23,498 publications

(7,578 citation statements)

References 8 publications

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“…These were fixed in 6% glutaraldehyde (Sabatini, Bensch and Barrnett, 1963), post-fixed in osmium tetroxide (Palade, 1952), and embedded in Araldite (Durcupan ACM Fluka) according to Luft (1961). Uranyl acetate was used during dehydration (Watson, 1958) and lead citrate applied to the sections (Reynolds, 1963) For light microscopy, several formalinfixed paraffin-embedded blocks were made, some prepared from areas adjacent to those from which the specimens for electron microscopy had been chosen. Sections were stained with H. and E. and also with the method developed for lung tumours by Kreyberg and Jareg (Kreyberg, 1967).…”

Section: Methodsmentioning

confidence: 99%

Human Peripheral Lung Tumours: Light and Electron Microscopic Correlation

Mollo¹,

Canese²,

Campobasso³

1973

Br J Cancer

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Thirteen human peripheral lung tumours have been studied in both light and electron microscopy. They were classified as epidermoid carcinoma, mucus-secreting cell adenocarcinoma, and alveolar cell adenocarcinoma, the latter made up of granular pneumocytes. Alveolar cell cancer, as defined by ultrastructural features, could assume different gross histological patterns in light microscopy, and therefore electron microscopy is required for its identification. Since neither squamous nor mucous metaplasia was observed in any alveolar cell tumour, it is tentatively suggested that all peripheral lung tumours which lack these features may be derived from granular pneumocytes, irrespective of whether they appear to be adenocarcinomata or large cell carcinomata when examined by light microscopy. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig. 10 Fig. 11 Fig. 12 Fig. 13 Fig. 14

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Section: Methodsmentioning

confidence: 99%

Human Peripheral Lung Tumours: Light and Electron Microscopic Correlation

Mollo¹,

Canese²,

Campobasso³

1973

Br J Cancer

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“…EPON blocks with embedded tissue were then trimmed, using a Leica EM TRIM (Leica, Vienna, Austria), to the size of the corpus callosum. In the following, ultrathin sections were stained with an aqueous solution of 4% uranyl acetate followed by lead citrate 18. The pictures were taken in an unbiased random fashion with a Zeiss EM900 electron microscope (Zeiss, Oberkochen, Germany) using a side‐mounted 2k CCD camera (TRS, Waakirchen, Germany).…”

Section: Methodsmentioning

confidence: 99%

The Cannabinoid CB1/CB2 Agonist WIN55212.2 Promotes Oligodendrocyte Differentiation In Vitro and Neuroprotection During the Cuprizone‐Induced Central Nervous System Demyelination

et al. 2016

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SummaryAim and methodsDifferent types of insults to the CNS lead to axon demyelination. Remyelination occurs when the CNS attempts to recover from myelin loss and requires the activation of oligodendrocyte precursor cells. With the rationale that CB1 receptor is expressed in oligodendrocytes and marijuana consumption alters CNS myelination, we study the effects of the cannabinoid agonist WIN55212.2 in (1) an in vitro model of oligodendrocyte differentiation and (2) the cuprizone model for demyelination.ResultsThe synthetic cannabinoid agonist WIN55212.2 at 1 μM increased the myelin basic protein mRNA and protein expression in vitro. During cuprizone‐induced acute demyelination, the administration of 0.5 mg/kg WIN55212.2 confers more myelinated axons, increased the expression of retinoid X receptor alpha, and declined nogo receptor expression. Controversially, 1 mg/kg of the drug increased the number of demyelinated axons and reduced the expression of nerve growth factor inducible, calreticulin and myelin‐related genes coupling specifically with a decrease in 2′,3′‐cyclic nucleotide 3′ phosphodiesterase expression.ConclusionThe cannabinoid agonist WIN55212.2 promotes oligodendrocyte differentiation in vitro. Moreover, 0.5 mg/kg of the drug confers neuroprotection during cuprizone‐induced demyelination, while 1 mg/kg aggravates the demyelination process.

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“…Tissue blocks were dehydrated in graded ethanols, and embedded in Araldite using epoxypropane as transitional solvent. Ultrathin sections wAere cut on an LKB ultramicrotome, stained with alcoholic uranyl acetate and lead citrate (Reynolds, 1963) and viewed in a Philips 301 microscope.…”

Section: Methodsmentioning

confidence: 99%

The structure of tumours derived from mouse submandibular gland epithelium transformed in vitro

Franks¹,

Knowles²

1978

Br J Cancer

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Summary. The morphology and ultrastructure of 48 primary tumours established from 5 cell lines of adult mouse salivary gland epithelial cells transformed in vitro are described. Tumours from 4 of the cell lines were adenocarcinomas with a wide range of structural variation, and resembled human salivary gland carcinomas. The fifth cell line produced tumours with carcinomatous and sarcomatous elements.

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THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

Cited by 23,498 publications

References 8 publications

Human Peripheral Lung Tumours: Light and Electron Microscopic Correlation

Human Peripheral Lung Tumours: Light and Electron Microscopic Correlation

The Cannabinoid CB1/CB2 Agonist WIN55212.2 Promotes Oligodendrocyte Differentiation In Vitro and Neuroprotection During the Cuprizone‐Induced Central Nervous System Demyelination

The structure of tumours derived from mouse submandibular gland epithelium transformed in vitro

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