The Use of Mass Spectrometry for Characterization of Fungal Secretomes

Callegari, Eduardo; Navarrete, Mario

doi:10.5772/30908

Cited by 1 publication

(1 citation statement)

References 38 publications

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“…The formation of multi-enzymatic complexes has been reported in the thermophilic fungus Chaetomium spp . [ 118 ], and more recently for Trichoderma harzianum and Penicillium purpurogenum [ 49 , 50 , 119 ]. For the latter, secretome analysis carried out in native conditions led to the identification of different subunits, including glucan 1,4-α-glucosidase, β-1,6-glucanase, α- l -arabinofuranosidase, alcohol dehydrogenase, endo-1,4-β-xylanase, cell wall protein PhiA acetylxylan esterase 2 [ 49 , 119 ].…”

Section: Secretome For Targeted Bioprocesses: From Efficient Biomamentioning

confidence: 99%

Methodologies and Perspectives of Proteomics Applied to Filamentous Fungi: From Sample Preparation to Secretome Analysis

Bianco

Perrotta

2015

IJMS

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Filamentous fungi possess the extraordinary ability to digest complex biomasses and mineralize numerous xenobiotics, as consequence of their aptitude to sensing the environment and regulating their intra and extra cellular proteins, producing drastic changes in proteome and secretome composition. Recent advancement in proteomic technologies offers an exciting opportunity to reveal the fluctuations of fungal proteins and enzymes, responsible for their metabolic adaptation to a large variety of environmental conditions. Here, an overview of the most commonly used proteomic strategies will be provided; this paper will range from sample preparation to gel-free and gel-based proteomics, discussing pros and cons of each mentioned state-of-the-art technique. The main focus will be kept on filamentous fungi. Due to the biotechnological relevance of lignocellulose degrading fungi, special attention will be finally given to their extracellular proteome, or secretome. Secreted proteins and enzymes will be discussed in relation to their involvement in bio-based processes, such as biomass deconstruction and mycoremediation.

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