The Use of Mean Instead of Smallest Interspecific Distances Exaggerates the Size of the “Barcoding Gap” and Leads to Misidentification

Meier, Rudolf; Zhang, Guanyang; Ali, Farhan

doi:10.1080/10635150802406343

Cited by 470 publications

(395 citation statements)

References 46 publications

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“…This sharply contrasts with that calculated between R. maderensis and R. clavata (0.4 %). Meier et al (2008) considered that the lowest distance to the nearest neighbour might be a more appropriate cut-off. In this case, comparison with the levels of divergence between R. clavata and its close relatives R. straeleni and R. radula, may be informative.…”

Section: Cytochrome Oxidase I Analysismentioning

confidence: 99%

Resolving taxonomic uncertainty in vulnerable elasmobranchs: are the Madeira skate (Raja maderensis) and the thornback ray (Raja clavata) distinct species?

et al. 2016

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Skates and rays constitute the most speciose group of chondrichthyan fishes, yet are characterised by remarkable levels of morphological and ecological conservatism. They can be challenging to identify, which makes monitoring species compositions for fisheries management purposes problematic. Owing to their slow growth and low fecundity, skates are vulnerable to exploitation and species exhibiting endemism or limited ranges are considered to be the most at risk. The Madeira skate Raja maderensis is endemic and classified as 'Data Deficient' by the IUCN, yet its taxonomic distinctiveness from the morphologically similar and more wide-ranging thornback ray Raja clavata is unresolved. This study evaluated the sequence divergence of both the variable control region and cytochrome oxidase I 'DNA barcode' gene of the mitochondrial genome to elucidate the genetic differentiation of specimens identified as R. maderensis and R.clavata collected across much of their geographic ranges. Genetic evidence was insufficient to support the different species designations. However regardless of putative species identification, individuals occupying waters around the Azores and North African Seamounts represent an evolutionarily significant unit worthy of special consideration for conservation management.

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Section: Cytochrome Oxidase I Analysismentioning

confidence: 99%

Resolving taxonomic uncertainty in vulnerable elasmobranchs: are the Madeira skate (Raja maderensis) and the thornback ray (Raja clavata) distinct species?

et al. 2016

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“…As sequências consenso dos "DNA barcodes", COI-5P e UPA, de todas as amostras, foram alinhadas em uma matriz no programa MEGA 5.0 (Tamura et al, 2011) interespecífica mínima e intraespecífica máxima das espécies (Freshwater et al, 2010;Meier et al, 2008).…”

Section: Alinhamento E Análises Molecularesunclassified

Diversidade de Gelidiales (Rhodophyta) baseada em marcadores moleculares e estudos morfoanatômicos para região Sudeste do Brasil

Iha¹

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“…So the inter-and intraspecific divergences of ITS were assessed. The intraspecific variations of Dendrobium plants in this study ranged from a minimum 0.000 (D. officinale, D. moniliforme, D. salaccense, D. bellatulum, D. primulinum, D. trigonopus, D. infundibulum, D. crystallinum, D. christyanum, D. jenkinsii, D. loddigesii, D. nobile, D. falconeri, D. sinense, D. gibsonii, D. catenatum, D. lituiflorum, D. tosaense, D. monticola Intra-and interspecific variations of Dendrobium plants were characterized by six metrics (average intraspecific distance, coalescent depth, theta, average interspecific distance, the minimum inter-specific distance and theta prime) (Meier et al, 2008). The results indicated that intraspecific variations of ITS sequences were relatively smaller and inter-specific divergences were very obvious (Table 2).…”

Section: Intra-and Inter-specific Genetic Divergencementioning

confidence: 99%

Authentication of Chinese Herbal Medicines Dendrobium Species and Phylogenetic Study Based on nrDNA ITS Sequence

Wang¹

2018

IJAB

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Dendrobium plants are one of the most valuable traditional Chinese medicinal herbs. Because of excessive exploitation and habitat degradation, Dendrobium species are endangered. Moreover, during no flowering stages, it is very difficult and even impossible to distinguish Dendrobium species based on morphological characteristics. Therefore, exploiting other DNA barcodes as complementary molecular marker for distinguishing Dendrobium spp. are necessary. Hence, developing an uncomplicated and exact method for authenticating Dendrobium spp. is imperative. In this study, nuclear ribosomal DNA internal transcribed spacer (ITS) sequences of 320 samples from 73 Dendrobium spp. were analyzed. The average intra-and inter-specific genetic divergences were 0.007 and 0.220, respectively. The TaxonGap method indicated that between-species variation of about 80.9% of the species were higher than within-species variation. The neighbor-joining tree showed that 73 Dendrobium spp. were divided into four major clades. Phylogenetic relationships between Dendrobium spp. supported traditional morphological methods and previously published molecular data. This study indicated that ITS region was not only suitable for species identification, but also for phylogenetic analysis of Dendrobium. However, it was also found that ITS could not readily resolve all species determination problems in Dendrobium.

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The Use of Mean Instead of Smallest Interspecific Distances Exaggerates the Size of the “Barcoding Gap” and Leads to Misidentification

Cited by 470 publications

References 46 publications

Resolving taxonomic uncertainty in vulnerable elasmobranchs: are the Madeira skate (Raja maderensis) and the thornback ray (Raja clavata) distinct species?

Resolving taxonomic uncertainty in vulnerable elasmobranchs: are the Madeira skate (Raja maderensis) and the thornback ray (Raja clavata) distinct species?

Diversidade de Gelidiales (Rhodophyta) baseada em marcadores moleculares e estudos morfoanatômicos para região Sudeste do Brasil

Authentication of Chinese Herbal Medicines Dendrobium Species and Phylogenetic Study Based on nrDNA ITS Sequence

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