Medicinal plants contain bioactive compounds that boost fish immunity and offer an effective chemical compound alternative. The present experiment aimed to study the effect of Tinospora cordifolia leaf powder on common carp (Cyprinus carpio), evaluating growth performance, hematobiochemical indices, and serum antioxidant-enzymes activity. The fish species were fed at different levels 0, 2, 4, and 6g/ kg of T. cordifolia leaf powder for 60 days. The results showed that the diet supplemented with 6g/ kg improved significantly (P< 0.05) the growth performance parameters, red blood cell count, hematocrit, hemoglobin, total protein, albumin, and globulin. In addition, the levels of superoxide dismutase, glutathione peroxidase, and lysozyme also rose significantly (P< 0.05). On the other hand, glucose levels decreased significantly (P< 0.05) with T. cordifolia supplementation. This research indicates that T. cordifolia leaf powder is a viable feed additive for improving the growth performance and general health of common carp. Consequently, it would assist in enhancing aquaculture's sustainability. responsible for its pharmacological effects (Sajith & Farhan, 2022). Tinospora leaves are 5-10cm long, heart-shaped, and rich in calcium, phosphorus, and protein (Sinha et al., 2004; Choudhary et al., 2014). It has been reported that T. cordifolia leaf extractsupplemented diets improved growth performance, survival, and haemato-biochemical profiles in catfish Pangasius sutchi fingerlings (Latha et al., 2020). Similarly, El Basuini et al. (2022) found that dietary T. cordifolia improves growth performance, antioxidative capacity, immune response, and resistance against hypoxia stress in the Nile tilapia. The current study aimed to evaluate the effects of T. cordifolia leaf powder on growth performance, hemato-biochemical parameters, and antioxidant-enzyme activities in C. carpio, an economically important farmed fish species in Iraq.
MATERIALS AND METHODS
Rearing system and experimental conditionThis study was conducted at the University of Basrah, College of Agriculture, Department of Fisheries and Marine Resources, using recirculating aquaculture systems. The system comprises two rearing units, each with six plastic containers with a capacity of 30 liters, oxygen pumps, mechanical and biological filters with a flow rate of 3 liters per minute. Daily monitoring parameters included water temperature, dissolved oxygen, pH, salinity, and weekly ammonia levels measurement. C. carpio fingerling was supplied from the Fish Farming Unit of the University of Basrah, then immersed in a salt solution of NaCl at a dosage of 5g/ L for 1.0min to prevent external parasites. Approximately, 120 fish with an average weight of 15.13±0.01g were distributed among twelve tanks, ten fish in each tank, and three replicates for each of the four treatments. Fish were fed twice daily at 2% of their body weight during the two-week acclimatization and 3% of their body weight during the experiment.
Diet preparationA commercial pallet was used as a basal ...