The use of microorganisms in l-ascorbic acid production

Bremus, Christoph; Herrmann, U.; Bringer-Meyer, Stephanie; Sahm, Hermann

doi:10.1016/j.jbiotec.2006.01.010

Cited by 154 publications

(88 citation statements)

References 59 publications

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“…However, unlike the ACCO-catalyzed reaction, the rate of 1-undecene formation under single turnover conditions in the absence of ascorbate was not significantly lower than the rate under multiple turnover conditions in the presence of ascorbate, and nearly a stoichiometric amount of 1-undecene was formed in the absence of ascorbate, suggesting that ascorbate is not needed for O 2 activation by UndA-Fe(II) to catalyze 1-undecene formation (29,30). In addition, ACCO is found in plants, which naturally produce ascorbate, but most Pseudomonas species lack the ability to synthesize ascorbate (31,32), suggesting that ascorbate is probably not the cognate-reducing cosubstrate for UndA. We propose that in the in vitro assays, ascorbate acts mainly as a reductant after 1-undecene formation by restoring the ferrous species, and this role of ascorbate has previously been reported (SI Appendix, Fig.…”

Section: Discussionmentioning

confidence: 99%

Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase

Rui

Zhu

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

189

233

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Aliphatic medium-chain 1-alkenes (MCAEs, ∼10 carbons) are "drop-in" compatible next-generation fuels and precursors to commodity chemicals. Mass production of MCAEs from renewable resources holds promise for mitigating dependence on fossil hydrocarbons. An MCAE, such as 1-undecene, is naturally produced by Pseudomonas as a semivolatile metabolite through an unknown biosynthetic pathway. We describe here the discovery of a single gene conserved in Pseudomonas responsible for 1-undecene biosynthesis. The encoded enzyme is able to convert medium-chain fatty acids (C10-C14) into their corresponding terminal olefins using an oxygen-activating, nonheme iron-dependent mechanism. Both biochemical and X-ray crystal structural analyses suggest an unusual mechanism of β-hydrogen abstraction during fatty acid substrate activation. Our discovery unveils previously unidentified chemistry in the nonheme Fe(II) enzyme family, provides an opportunity to explore the biology of 1-undecene in Pseudomonas, and paves the way for tailored bioconversion of renewable raw materials to MCAE-based biofuels and chemical commodities.urging energy consumption and environmental concerns have stimulated interest in the production of chemicals and fuels through sustainable and renewable approaches. Medium-chain 1-alkenes (MCAEs) are of particular interest because they are "drop-in"-ready next-generation fuels with superior properties such as low freezing point compared with long-chain diesels, high energy content compared with short-chain fuels, easy product recovery due to insolubility in water, and compatibility with the existing engine systems and transportation infrastructure (1, 2). Because of a readily derivatized terminal functionality, MCAEs are also valuable precursors to commodity chemicals such as lubricants, pesticides, polymers, and detergents (3, 4). Biological production of MCAEs from renewable resources holds promise for mitigating dependence on fossil hydrocarbons. Although MCAEs are naturally produced by diverse species as semivolatile metabolites (5, 6), little is known about the genetic and molecular basis for MCAE biosynthesis. Elucidation of MCAE biosynthetic pathway will serve as the basis for engineering efforts to establish bioprocesses for producing MCAE-based biofuels and chemical commodities from renewable resources.1-Undecene, an MCAE with 11 carbons, was identified as a biomarker of Pseudomonas aeruginosa, one of the most significant human pathogens (7-10). However, the biology of this characteristic semivolatile metabolite in P. aeruginosa remains enigmatic, and the biosynthetic pathway of 1-undecene has not previously been explored. It was also reported that some species of Pseudomonas produce 1-undecene, whereas some species do not (8), inspiring us to use a comparative genomics approach to reveal the genetic basis for 1-undecene biosynthesis (11). It is notable that one of the major challenges for MCAE biosynthetic study is the detection and quantification of MCAE production. MCAEs, such as 1-undecene, are only ...

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Section: Discussionmentioning

confidence: 99%

Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase

Rui

Zhu

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

189

233

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“…13 C and 1 H NMR spectroscopy on isotopically-labeled glucose reveals that the glucose-sorbose isomerization occurs through an intramolecular C5-C1 hydride shift. Sorbose sugar is largely used in the synthesis of L-ascorbic acid, which is a form of vitamin C. 42 …”

Section: 1-sugar Isomerizationmentioning

confidence: 99%

State of the art of Lewis acid-containing zeolites: lessons from fine chemistry to new biomass transformation processes

2014

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“…Moreover, high added value compounds can be extracted from microalgae, such as fatty acids (linolenic, arachidonic, eicosapentaenoic, docosahexaenoic acids, etc.) (Cardozo, et al, 2007 andValencia et al, 2007) pigments (carotenoids and ficobiliproteins), biochemically stable isotopes (Chisti 2007 andBorowitzka, et al, 1991) and vitamins such as biotin (Baker et al, 1981) vitamins C (S.A. Survase et al, 2006) and vitamin E (Running et al, 2002 andC. Bremus et al, 2006) besides some metabolites appear to have some pharmacological activities, among others the anticholesterolemic, antitumoral, immune modulatory, antibacterial and antimycotic ones.…”

Section: Introductionmentioning

confidence: 99%