The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance

Abstract

Fusarium head blight (FHB) is a barley disease, which occurs every year in various areas of barley cultivation all over the world and the increasing incidence has been confirmed in the Czech Republic also during the last years. We aimed to emply AFLP (Amplified fragment length polymorphism) and SSR (Single sequence repeats) markers to describe diversity among breeding lines with a sufficient level of resistance towards FHB and to find marker(s) associated with the analysed traits. The number of eight accessions including five expected resistance donors and three sensitive lines were tested in the field and laboratory. The field values and the amount of deoxynivalenol were positively correlated (r = 0.92). The laboratory test and content DON manifested also a high correlation (r = 0.73). Several DH lines developed from androgenetic barley progenies of the F1 hybrids between the susceptible line PI 383933 and resistant line PEC 210 or the susceptible line PI 383933 and resistant cultivar Chevron were found resistant towards Fusarium infection in both the field and laboratory tests. Low infestation was found at line DH 37 from combination Chevron × PI 383933 and lines DH48, DH49, DH50 and DH55 from the combination PEC 210 × PI 383933. Cluster analyses based on 68 AFLP and 18 SSR markers demonstrate a genetic relationship among parental genotypes and DH lines. Some DH lines combined a sufficient degree of resistance against FHB and extract content (basic parameters malting quality). Statistically significant differences in malt-extract values were observed between groups of the DH lines possessing and not possessing the AFLP marker CAA/AGC 341bp. The markers will be further evaluated and optionally used for MAS.

Genetic reticulation and interrelationships amongcitrullusspecies as revealed by joint analysis of shared AFLPs and species-specific SSR alleles

“…The current study indicated that the joint analysis of AFLP and SSR is very effective for phylogenetic analysis of Citrullus spp. Studies such as Špunarová et al (2005) in barley, Gillaspie et al (2005) in Vigna , Maluf et al (2005) in Coffea , Saini et al 2004 in rice, Perumal et al (2007) in sorghum are few examples of copious published works, where joint analysis of AFLP and SSR datasets proved that these markers analyzed together have unprecedented utility for phylogenetic studies. Instead of generating a particular sequence-specific tree that does not necessarily reflect the true species tree, especially among the closely related and potentially interbreeding species such as Citrullus spp., where reticulate evolution might have occurred, the simultaneous analysis of many loci representing the whole genome has the potential to generate true phylogenies.…”

Exploring Wild Hordeum spontaneum and Hordeum marinum Accessions as Genetic Resources for Fungal Resistance

“…Except for one genotype (01C0509112), all of the H. marinum accessions were moderately resistant to FHB in the greenhouse tests, probably reflecting their rather humid place of origin, where the pathogen spreads easily and where plants have to cope with pathogen attack, exemplifying their adaptation to environmental factors, as indicated previously [ 45 , 46 , 47 ]. SSR markers linked to disease resistance in barley did not amplify the same alleles on the studied loci that we found in the resistant barley cultivars Chevron and Frederikson [ 48 ]. The size variability of SSRT alleles was considerable.…”