The use of monoamine oxidase inhibition to estimate megakaryocyte-platelet regeneration time (MPRT)

Chamberlain, Kevin G.; Tong, Michael; Chiu, Edmond; Penington, D. G.

doi:10.1016/0049-3848(88)90245-9

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…Furthermore, Soslau (36) reported that cytochrome oxidase activity did not change in platelets stored in vitro for up to eight days. In addition, the recovery of platelet MAO activity after irreversible inhibition shows a basically linear pattern with a recovery time similar to the platelet lifespan suggesting that loss of MAO activity during the platelet lifespan is negligible (37,38). The ultrastructural data of Corash et al (34) might be compatible with the present marker enzyme results if low density platelets are shown to have smaller mitochondria than high density platelets.…”

Section: Discussionsupporting

confidence: 72%

Monoamine Oxidase and Other Mitochondrial Enzymes in Density Subpopulations of Human Platelets

Chamberlain

Penington

1988

Thromb Haemost

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SummaryNormal human platelets have been separated according to density on continuous Percoll gradients and the platelet distribution divided into five fractions containing approximately equal numbers of platelets. The mean volumes and protein contents of the platelets in each fraction were found to correlate positively with density while the protein concentration did not differ significantly between the fractions. Four mitochondrial enzymes (monoamine oxidase, glutamate dehydrogenase, cytochrome oxidase and NADP-dependent isocitrate dehydrogenase) were assayed and their activities per unit volume were found to increase in a very similar monotonie fashion with platelet density. When MAO and GDH were assayed on the same set of density fractions the correlation between the two activities was very high (r = 0.94–1.00, p <0.001) and a similar close correlation was found between MAO and ICDH. The results support the hypothesis that high density platelets either have a higher concentration of mitochondria or have larger mitochondria than low density platelets.

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Section: Discussionsupporting

confidence: 72%

Monoamine Oxidase and Other Mitochondrial Enzymes in Density Subpopulations of Human Platelets

Chamberlain

Penington

1988

Thromb Haemost

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“…On day 7, normal parameters were obtained by others [20]. These findings may indicate a faster recovery of thrombocytic function after aspirin ingestion [21] than previously reported [5,22]. This may diminish the risk of bleeding after 4 days in patients with concomitant anticoagulant medication [23].…”

Section: Single High Dose Of Aspirinsupporting

confidence: 74%

“…Furthermore, the effect on platelets depends on new platelet production. With the PFA values returning to baseline levels after 4 days, there may be an indicator that platelet regeneration time is much shorter than previously quoted [22].…”

Section: Single High Dose Of Aspirinmentioning

confidence: 70%

Duration of effects of aspirin on platelet function in healthy volunteers: an analysis using the PFA-100

Konrad¹,

Schuepfer²,

Gerber³

et al. 2006

Journal of Clinical Anesthesia

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Properties of the exchangeable splenic platelets released into the circulation during exercise‐induced thrombocytosis

1990

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The human spleen normally retains about one-third of the body's platelets in an exchangeable pool which can be released into the circulation by alpha-adrenergic stimulation. Some previous investigators concluded that the splenic platelet population was enriched in a subpopulation of large, young, dense platelets (megathrombocytes) but more recent research suggests that platelet size, age, and density are largely independent variables. In this investigation the properties of the splenic platelets were studied after their release into the circulation by acute strenuous exercise in 11 normal subjects. The exercise caused a rise in mean platelet count from 245 +/- 49 to 328 +/- 71 x 10(9)/L--a net increase of 24 +/- 6% after correction for haemoconcentration. The mean platelet volume (MPV) of citrated platelets increased from 6.38 +/- 0.78 to 6.59 +/- 0.68 fL after exercise (P less than 0.01)--a rise of 3.7 +/- 4.1% suggesting that the MPV of the splenic platelet population was about 20% greater than that of the normal circulating population. The age distribution of the platelets was studied by measuring the platelet monoamine oxidase (MAO) activity several days after irreversible inhibition by tranylcypromine, when the young platelets had normal MAO activity but the older platelets had only 20% of normal activity. The mean platelet MAO activity did not change after exercise, indicating that the age distributions of the circulating and splenic populations were very similar. The platelet contents of several putative markers of platelet age (sialic acid, serotonin, beta-thromboglobulin, beta-N-acetylglucosaminidase) were also unchanged after exercise. Modal platelet density decreased slightly but not significantly after exercise. The splenic platelet population has a larger MPV but appears to have similar age and density distributions to the basal circulating population.

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The use of monoamine oxidase inhibition to estimate megakaryocyte-platelet regeneration time (MPRT)

Cited by 7 publications

References 33 publications

Monoamine Oxidase and Other Mitochondrial Enzymes in Density Subpopulations of Human Platelets

Monoamine Oxidase and Other Mitochondrial Enzymes in Density Subpopulations of Human Platelets

Duration of effects of aspirin on platelet function in healthy volunteers: an analysis using the PFA-100

Properties of the exchangeable splenic platelets released into the circulation during exercise‐induced thrombocytosis

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