The Use of Nanocomposite Coatings with Various Physicochemical Properties in Tissue Engineering

Abstract

We studied the effect of nanocomposite coatings with various physicochemical properties on the structural and functional properties (adhesion potential, phenotype, gene expression) of mesenchymal stem cells. Of all tested nanocoatings (Al2O3, ZrO2, Ta2O5), oxide coating Al2O3 enriched in vitro monolayer bone marrow cell culture with cells carrying mesenchymal stem cells phenotype markers and stimulated expression of ido gene, which can confer new therapeutic potencies to these cells and extend their application in clinical practice.

Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings

“…Their application enabled to enrich during two passages the total bone marrow pool with the cells possessing MSCs markers, and the expression rate of ido gene transcripts in the cells as well [13,26]. These findings have confirmed the effect of the culture surface coating on MSCs' functional potential, in particular, the IDO production, determining an immune suppressive activity of these cells.…”

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“…Our preliminary studies for MSCs culturing involved the Al 2 O 3 oxide coatings. Their application enabled to enrich during two passages the total bone marrow pool with the cells possessing MSCs markers, and the expression rate of ido gene transcripts in the cells as well [13,26]. These findings have confirmed the effect of the culture surface coating on MSCs' functional potential, in particular, the IDO production, determining an immune suppressive activity of these cells.…”