The use of naturally occurring selectively isolated bacteria for inhibiting paraffin deposition

Lazăr, Ioan; Voicu, Anca M.; Nicolescu, Cristian; Mucenica, D; Dobrota, S.; Petrisor, Ioana G.; Stefanescu, M.; Sandulescu, L.

doi:10.1016/s0920-4105(98)00065-5

Cited by 63 publications

(29 citation statements)

References 5 publications

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“…Microbial processes to treat wax deposition problems involve two main mechanisms. The first one is in situ conversion of long-chain hydrocarbons to short-chain hydrocarbons by hydrocarbondegrading microorganisms (Brown et al 2005;Lazar et al 1999). The second one is alteration of the physical properties of crude oil by the products of emulsifier-producing or biosurfactant-producing bacteria (Banat 1995;Banat et al 2010; Barkay et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir

Zhang

She

et al. 2011

Appl Microbiol Biotechnol

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Based on preliminary investigation of microbial populations in a high pour-point oil reservoir, an indigenous microbial enhanced oil recovery (MEOR) field trial was carried out. The purpose of the study is to reveal the impact of the indigenous MEOR process on microbial community structure in the oil reservoir using 16Sr DNA clone library technique. The detailed monitoring results showed significant response of microbial communities during the field trial and large discrepancies of stimulated microorganisms in the laboratory and in the natural oil reservoir. More specifically, after nutrients injection, the original dominant populations of Petrobacter and Alishewanella in the production wells almost disappeared. The expected desirable population of Pseudomonas aeruginosa, determined by enrichment experiments in laboratory, was stimulated successfully in two wells of the five monitored wells. Unexpectedly, another potential population of Pseudomonas pseudoalcaligenes which were not detected in the enrichment culture in laboratory was stimulated in the other three monitored production wells. In this study, monitoring of microbial community displayed a comprehensive alteration of microbial populations during the field trial to remedy the deficiency of culture-dependent monitoring methods. The results would help to develop and apply more MEOR processes.

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Section: Introductionmentioning

confidence: 99%

Impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir

Zhang

She

et al. 2011

Appl Microbiol Biotechnol

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“…Since precipitation cannot be completely halted, necessary measures should be taken in order to remove deposition. In general, the following methods are used to resolve the precipitation-induced problems: mechanical, thermal, chemical (solvent application), electromagnetic, chemical surfactants, crystal modifiers, ultrasound, and biological degradation [5][6][7]. These methods have been used so far to remove precipitation, including the common method of organic aromatic solvents such as toluene and hot xylene which exert numerous environmental problems due to their carcinogenesis.…”

Section: Common Ways Of Coping With Precipitationmentioning

confidence: 99%

Protocol for Isolation, Screening, and Cultivation of Asphaltene-Degrading Microorganism

Zamir

Tavassoli

Shojaosadati

2015

Springer Protocols Handbooks

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Precipitation of heavy hydrocarbons, especially wax and asphaltenes, is a major problem in oil-related industries. The presence of asphaltenes results in higher viscosity, emulsion formation, coke production, and consequently clogging of pipelines. These compounds contain metals such as iron, nickel, and vanadium and hence are poisonous for catalysts in refinery processes. This has led to a strong incentive to find microorganisms which are able to cleave asphaltene compounds and thus promote facile exploitation of these resources.

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“…Lazar et al (1999) achieved 95.3% petroleum hydrocarbon degradation from paraffinic crude oil at 28°C in 10 to 12 days by using a consortium of 15 bacterial isolates. The reduction in wax appearance temperature and heavy hydrocarbon fractions by biodegradation of paraffinic hydrocarbons using Pseudomonas and Actinomyces species was noticed (Etoumi, 2007).…”

Section: Biodegradation Of Hydrocarbonmentioning

confidence: 99%

In vitro screening for oil degrading bacteria and evaluation of their biodegradation potential for hydrocarbon

ndash¹,

Tarrs²,

Shahaby³

et al. 2012

Afr. J. Microbiol. Res.

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Twenty seven potential hydrocarbon degrader bacterial isolates were isolated from soil that has been exposed to crude petroleum oil. The hydrocarbon degradation potentialities of these isolates were assessed against n-tetradecane. Four isolates (AT3, AT5, AT11 and AT15) were the most potent isolates. These isolates were identified via morphological, biochemical and 16S-rRNA gene sequencing techniques as (Pseudomonas stutzeri, Bacillus thuringiensis, Bacillus pumilus and Bacillus cereus), respectively. AT3 isolate was the highest n-tetradecane degrader. This isolate exhibited specific growth rate in Bushnell-Hass medium amended with hydrocarbon, n-tetradecane of 1.213 h -1 and growth yield of 1.311 g cells g hydrocarbon -1. An efficient biodegradation up to 90% was performed by AT3 bacterial isolate. Genetic fingerprinting was carried out using RAPD-PCR and SDS-PAGE methods to characterize and conduct phylogenetic relationship among the four most potent isolates. Thirty six specific markers were obtained and used to characterize the four studied isolates. Different specific markers for AT3 isolate were generated. The closest genetic distance was found between isolate AT11 and AT15 (0.55), while the lowest genetic similarity was between AT3 and AT5 (0.41). The introduced markers could be used for tracking the most potent isolates.

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The use of naturally occurring selectively isolated bacteria for inhibiting paraffin deposition

Cited by 63 publications

References 5 publications

Impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir

Impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir

Protocol for Isolation, Screening, and Cultivation of Asphaltene-Degrading Microorganism

In vitro screening for oil degrading bacteria and evaluation of their biodegradation potential for hydrocarbon

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