The use of paraffin wax metabolism in the speciation of mycobacterium avium-intracellulare

Ollar, Robert A.; Dale, Jeremy W.; Felder, Mitchell S.; Favate, A.

doi:10.1016/0041-3879(90)90056-e

Cited by 17 publications

(9 citation statements)

References 7 publications

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“…Nontuberculous mycobacteria, primarily rapidly growing mycobacteria, have been shown to be responsible for degradation of a wide variety of novel compounds, including acetone (61,62), alkanes (368), benzene (61,62), chlorinated phenolics (197), dioxane (61,62), ethene (200), fluoranthene (48), humic and fulvic acids (291), morpholine (59), nitropyrene (212), paraffin (378), pentachlorophenol (506), phenanthrene (48,190), polycyclic aromatic hydrocarbons (529), polyhalogenated phenolics (196,376), propane (84), pyrene (212), pyridines (including isonicotinate) (298,299), styrene (61,62), trichloroethylene (520), trinitrotoluene (518), uric acid (148), and vinyl chloride (199). In addition, nontuberculous mycobacteria are capable of metabolizing and transforming sterols and are used in industrial processes for sterol production (231,249,451,519).…”

Section: Ecology and Physiology Of Nontuberculous Mycobacteriamentioning

confidence: 99%

Epidemiology of infection by nontuberculous mycobacteria

1996

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Section: Ecology and Physiology Of Nontuberculous Mycobacteriamentioning

confidence: 99%

Epidemiology of infection by nontuberculous mycobacteria

1996

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“…In contrast, M. tuberculosis is not found in the environment but is a typical and notorious example of the slow-growing pathogenic Mycobacteria. It is not able to grow on the paraffin-coated slides used in the so-called paraffin baiting method, unlike P. aeruginosa PAO1 and Mycobacterium avium (30,32). P. rugosa NRRL B-2295 was isolated from cow rumen and was selected because it is a useful biocatalyst that converts cumene to 2-phenyl-1-propionic acid (22) if it is grown on n-alkanes.…”

Section: Selection Of Strainsmentioning

confidence: 99%

Functional Analysis of Alkane Hydroxylases from Gram-Negative and Gram-Positive Bacteria

et al. 2002

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We have cloned homologs of the Pseudomonas putida GPo1 alkane hydroxylase from Pseudomonas aeruginosa PAO1, Pseudomonas fluorescens CHA0, Alcanivorax borkumensis AP1, Mycobacterium tuberculosis H37Rv, and Prauserella rugosa NRRL B-2295. Sequence comparisons show that the level of protein sequence identity between the homologs is as low as 35%, and that the Pseudomonas alkane hydroxylases are as distantly related to each other as to the remaining alkane hydroxylases. Based on the observation that rubredoxin, an electron transfer component of the GPo1 alkane hydroxylase system, can be replaced by rubredoxins from other alkane hydroxylase systems, we have developed three recombinant host strains for the functional analysis of the novel alkane hydroxylase genes. Two hosts, Escherichia coli GEc137 and P. putida GPo12, were equipped with pGEc47⌬B, which encodes all proteins necessary for growth on medium-chain-length alkanes (C 6 to C 12 ), except a functional alkane hydroxylase. The third host was an alkB knockout derivative of P. fluorescens CHA0, which is no longer able to grow on C 12 to C 16 alkanes. All alkane hydroxylase homologs, except the Acinetobacter sp. ADP1 AlkM, allowed at least one of the three hosts to grow on n-alkanes.

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“…from NTM (19,20). Kilburn and Kubica further modified the niacin test using strip impregnation (21).Another available method, paraffin slide culture technique for identification of NTM requires 7 days (2,22,23). The lag phase between clinical presentation and confirmed diagnosis is crucial period, as delay in TB treatment results in rapid spread of the disease.…”

Section: Discussionmentioning

confidence: 99%

Potential of mycobacterial excretory secretory protein antigens (sevatb ES-31, ES-43, EST-6 and ES-20) as biomarkers to detect Mycobacterium tuberculosis bacilli

Anindita

Thamke

Mendiratta

et al. 2010

Indian J Clin Biochem

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There is a need for a simple and reliable method to identify Mycobacterium tuberculosis from nontuberculous mycobacteria (NTM). The utility of mycobacterial ES-31, ES-43, EST-6 or ES-20 antigen as a biomarker for differentiation of Mycobacterium tuberculosis bacilli from nontuberculous mycobacteria was explored using Fluorescein isothiocyanate conjugated antibodies against these antigens. Detection of these antigens was done from M.tb H(37)Ra and H(37)Rv DSS antigen. The presence of antigen in bacilli using FITC labelled antibody was indicated by green fluorescence on the cell surface while, its absence by no fluorescence under microscope. In M.tb H(37)Ra and H(37)Rv bacilli, fluorescence was observed on addition of FITC labelled anti ES-31 and anti ES-43 antibody; whereas no fluorescence was observed in case of EST-6 and ES-20 antibody conjugates. However all the antigens were detected in detergent soluble sonicate antigen of tubercle bacilli on addition of FITC conjugates. Fluorescence was not observed for ES-31, ES-43, EST-6 and ES-20 antigen in any of the tested NTM as well as in Escherichia coli. SEVA TB ES-31 and ES-43 may be used as biomarkers to distinguish M.tuberculosis bacilli from NTM.

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The use of paraffin wax metabolism in the speciation of mycobacterium avium-intracellulare

Cited by 17 publications

References 7 publications

Epidemiology of infection by nontuberculous mycobacteria

Epidemiology of infection by nontuberculous mycobacteria

Functional Analysis of Alkane Hydroxylases from Gram-Negative and Gram-Positive Bacteria

Potential of mycobacterial excretory secretory protein antigens (sevatb ES-31, ES-43, EST-6 and ES-20) as biomarkers to detect Mycobacterium tuberculosis bacilli

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