The use of pentoxifylline to improve motility of cryopreserved equine spermatozoa

Gradil, Carlos M.; Ball, B. A.

doi:10.1016/s0093-691x(00)00412-x

Cited by 34 publications

(32 citation statements)

References 11 publications

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“…Under the present experimental conditions, 4, 8, 16 and 32 mM pentoxifylline added to the freezing extender decreased the postthaw motility and the viability of spermatozoa. This deleterious effect has also been observed in ovine (Maxwell et al 1995) and equine (Gradil & Ball 2000) species. Moreover, a detrimental effect of pentoxifylline on human spermatozoa was also observed at a concentration of 10 mM (Tournaye et al 1994) suggesting a concentration-dependent toxic effect when the substance is added to semen before freezing.…”

Section: Discussionmentioning

confidence: 66%

“…Moreover, pentoxifylline appears to be useful for improving the motility of spermatozoa in suboptimal human (Paul et al 1995) and equine ejaculates (Gradil & Ball 2000). Therefore, in the present study we investigated the effect of pentoxifylline added to the post-thaw extender on the survival and IVF rate of spermatozoa from ejaculates classified as 'good' or 'poor', according to their freezability characteristics.…”

Section: Discussionmentioning

confidence: 95%

“…It has been also used to enhance the motility of fresh and frozen spermatozoa from a number of domestic animals (Maxwell et al 1995, Stachecki et al 1995, Koutsarova et al 1997, Gradil & Ball 2000, Numabe et al 2001, Huang et al 2003. However, to the best of our knowledge, this is the first report on the effect of pentoxifylline on the quality of frozen-thawed boar spermatozoa and their ability to fertilise in vitro-matured porcine oocytes.…”

Section: Discussionmentioning

confidence: 96%

“…The range of pentoxifylline concentrations tested (from 1 to 32 mM) includes those that had been previously used with bovine (Numabe et al 2001), ovine (Maxwell et al 1995), equine (Gradil & Ball 2000, Marques et al 2002, human (Brennan & Holden 1995, Nassar et al 1999, Huang et al 2003 and boar (Glogowski et al 2002) spermatozoa.…”

Section: Experimental Designmentioning

confidence: 99%

“…Pentoxifylline, a methylxanthine derivate, is an inhibitor of phosphodiesterase. This substance has also been used to stimulate the motility of fresh and/or frozen-thawed human (Hammitt et al 1989, Brennan & Holden 1995, Nassar et al 1999, Huang et al 2003, equine (Gradil & Ball 2000, Marques et al 2002, bovine (Numabe et al 2001), ovine (Maxwell et al 1995), feline (Stachecki et al 1995) and canine spermatozoa (Koutsarova et al 1997). Also, we have observed that pentoxifylline is able to stimulate the motility of fresh boar spermatozoa (MA Gil, J Roca, JM Vasquez & EA Martinez, unpublished data; shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

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Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Gil¹,

Hernández²,

Roca³

et al. 2010

Reproduction

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This study evaluated whether pentoxifylline added to freezing and thawing extenders influenced the function of boar spermatozoa. In Experiment 1, pooled ejaculated sperm-rich fractions were frozen in 0.5 ml straws after dilution in extender supplemented with pentoxifylline to a final concentration of 0, 2, 4, 8, 16 or 32 mM. The addition of 4, 8, 16 and 32 mM pentoxifylline to the freezing extender significantly decreased the progressive and total motility of spermatozoa. The percentage of viable spermatozoa with intact acrosomes as well as the penetration rate and the efficiency of fertilisation were significantly lower in pentoxifylline-treated groups compared with the untreated control. In Experiment 2, a pool of three straws with 'good' post-thaw sperm quality parameters and another three straws with 'poor' sperm quality were diluted in extender with 0, 1, 2, 4, 8, 16 or 32 mM pentoxifylline. Post-thaw samples with both 'good' and 'poor' sperm quality with 0, 2, 4, 8 and 16 mM were used to assess IVF parameters. The addition of pentoxifylline to post-thaw extender did not improve the post-thaw motility or viability of spermatozoa compared with the control. The in vitro penetration was higher (P!0.05) than the control for oocytes fertilised with spermatozoa that were thawed and incubated in extender with 4, 8 and 16 mM pentoxifylline. However, no differences were observed in the efficiency of fertilisation. We conclude that pentoxifylline, as a supplement added to the freezing extender, has a deleterious effect and that it does not improve the survival or in vitro fertilising efficiency of frozen-thawed boar spermatozoa when added after thawing.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 96%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pentoxifylline added to freezing or post-thaw extenders does not improve the survival or in vitro fertilising capacity of boar spermatozoa

Gil¹,

Hernández²,

Roca³

et al. 2010

Reproduction

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Cryopreservation of sperm ofXenopus laevis andXenopus tropicalis

Sargent

Mohun

2005

genesis

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Now that transgenic strains of Xenopus laevis and X. tropicalis can be generated efficiently and with genomic sequence resources available for X. tropicalis, early amphibian development can be studied using integrated biochemical and genetic approaches. However, housing large numbers of animals generated during genetic screens or produced as novel transgenic lines presents a considerable challenge. We describe a method for cryopreserving Xenopus sperm that should facilitate low maintenance, long-term storage of male gametes. By optimising the cryoprotectant, the rates of cooling and thawing, and conditions for fertilisation, sperm from the equivalent of one-eighth of a X. laevis testis or of two X. tropicalis testes have been cryopreserved and used to fertilise eggs of both species after thawing. Sperm undergo a substantial loss of viability during a freeze-thaw cycle, but sufficient survive to fertilise eggs. Gametes of mutagenised frogs are being stored in connection with a screen for developmental mutations.

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