The use of pimaricin as an additive to improve the aerobic stability of silage

Abstract

Pimaricin was added to samples from each of five crop species to give the following nominal rates of application: 0, 0.01 I , 0.022, 0.034, 0.045, 0.09 and 0.27 kg t fresh weight and the materials were ensiled in 100 g and 10 kg fresh weight quantities.Pimaricin applied at ensiling at 0.01 1 or 0.022 kg t 1 reduced aerobic deterioration in three of the five crops-ryegrass, lucerne and tall fescue; a much higher rate of 0.27 kg t was required with silages made from red clover and maize. This beneficial effect occurred even though only from 1-66?; of the pimaricin initially applied was recovered after the period of anaerobic storage in the silos. Pimaricin had no apparent effect on the course of fermentation nor on the composition of the silages, all of which were well preserved.

Effects of natamycin and <i>Lactobacillus buchneri</i> on the fermentative process and aerobic stability of maize silage

“…Furthermore, Schmidt et al (2012) did not find a decrease in fermentative losses in natamycin treated maize silage. However, Woolford et al (1980) found a decrease of aerobic deterioration of maize silage after natamycin treatment but only at a high application rate (270 g/t). Therefore, the combination of additives may reduce the amount of used additives simultaneously increasing the recovery of DM from the final product.…”

“…In the present study no silage treatment showed efficient control of yeast growth after five days of aerobic exposure. Woolford et al (1980) found 34.6 and 3.8% of natamycin recovery rates after 7 and 100 days of fermentation, respectively. It suggests that natamycin acts only during the first stage of the forage fermentation.…”

“…A decrease in yeast counts and an increase in aerobic stability were observed in sugarcane silage treated with chemicals (sodium benzoate, potassium sorbate) or bacterial additives (Pedroso et al, 2008). Natamycin (pimaricin) has not yet been fully evaluated as an inhibitor of the growth of specific microorganisms during fermentation and aerobic exposure of silage, despite its potential to improve silage aerobic stability (Woolford et al, 1980). Natamycin is a bacteriocin obtained from Streptomyces natalensis culture.…”

“…Until now, the aerobic stability of silage was investigated after the forage treatment with only bacterial inoculant or only a chemical additive, there is no research examining the combination of these two types of silage additives (Knicky and Spörndly, 2011). Woolford et al (1980) observed a small decrease in the number of yeast and mould during the aerobic exposure of grass and maize silage with the addition of natamycin. New additives reducing fermentation losses while improving the aerobic stability of silage remain an important subject in forage conservation research (Muck et al, 2018).…”

“…New additives reducing fermentation losses while improving the aerobic stability of silage remain an important subject in forage conservation research (Muck et al, 2018). Based on current knowledge (Woolford et al, 1980;Knicky and Spörndly, 2011;Muck et al, 2018), studies on the association of L. buchneri with bacteriocins such as natamycin (Woolford et al, 1980, D'Urso et al, 1990, especially in low doses, still remain missing in the forage preservation literature. The current study combines L. buchneri and natamycin and assesses the effect of their mixture on the fermentative process and aerobic stability of maize silage to fill this gap.…”