The use of pinned specimens helps uncover patterns of genetic differentiation and signatures of selection in a wild pollinator

Samad-zada, Farida; Rehan, Sandra M.

doi:10.1016/j.gecco.2023.e02593

Cited by 3 publications

(1 citation statement)

References 77 publications

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“…In management practices, conservation units are often defined based on the determination of population distinctiveness, estimation of genetic diversity, test of population bottlenecks, measuring landscape connectivity and consideration of adaptive differentiation (Flanagan et al, 2018;Hendricks et al, 2020;Samad-zada & Rehan, 2023). Benefitted from the use of genomic SNPs and plastome data, we have identified three genetic lineages (i.e.…”

Section: Implication For Conservationmentioning

confidence: 99%

Genetic divergence and ecological adaptation of an eastern North American spring ephemeral Sanguinaria canadensis

Xia,

Luo,

Suyama

et al. 2024

Diversity and Distributions

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AimDescription of the driving forces for genetic divergence is important for understanding spatial pattern of biodiversity and development of conservation plans. Paleo‐climate, geographical barriers and habitat heterogeneity are considered to be the main influential factors; however, an integrative study is still lacking to reveal their interactions.LocationEastern North America.MethodsHere, we generated MIG‐seq SNPs of 403 Sanguinaria canadensis samples and seven plastome data. The methods of phylogeography and landscape genomics were applied to infer their genetic divergence, demography, species distribution modelling, ecological differentiation and local adaptation.ResultsWe identified three distinct genetic lineages corresponding to geographical distributions isolated by the Appalachian Mountains and the Mississippi River. Ecological niche modelling and population demographic inference demonstrated that the response of S. canadensis to Pleistocene climate changes was consistent with the pattern of southward contraction during the ice age and northward recolonization during the inter−/postglacial period. Isolation in multiple southern refugia was a key factor resulting in the genetic divergence, whereas secondary contact triggered by repeated range shifts allowed gene flow among different lineages. A greater effect of isolation‐by‐environment than isolation‐by‐distance was founded, which suggested heterogeneous environment was also a promotor of genetic differentiation. Candidate adaptive loci related to transposable elements were identified under the influence of divergent environmental selection. Ecological niche divergence also reflected adaptation to different environments.Main ConclusionsThis study revealed that genetic divergence has occurred within S. canadensis populations under the combined effect of paleo‐climate, geographical barriers and habitat heterogeneity, and emphasized the necessity of establishing different conservation units in future biological conservation and management work.

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Section: Implication For Conservationmentioning

confidence: 99%

Genetic divergence and ecological adaptation of an eastern North American spring ephemeral Sanguinaria canadensis

Xia,

Luo,

Suyama

et al. 2024

Diversity and Distributions

View full text Add to dashboard Cite

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Large carpenter bees show high dispersal in a tropical semi‐arid region susceptible to desertification

Brasil,

Araujo,

Brelsford

et al. 2024

Ecology and Evolution

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Desertification is a major threat to biodiversity in arid areas of the world, partly because many organisms in these regions already exist at or near the limits of their movement and physiology. Here, we used molecular data to investigate patterns of persistence and dispersal in an ecologically and economically important carpenter bee (Xylocopa grisescens Lepeletier) found throughout the semiarid Caatinga region of Brazil. We used a genome‐wide approach (double digest restriction‐site associated DNA, ddRAD) to gather genetic data from bees sampled from eight sites within a semiarid region subject to desertification in Northeastern Brazil. Across all populations, we observed a consistent heterozygosity and effective population size deficit along with low genetic differentiation. We did not find strong evidence of dispersal limitations caused by desertification in this study system despite data collection from sites up to 300 km distant. Thus, our data suggest that human‐mediated changes in the Caatinga, such as habitat loss, have impacted the population genetic patterns of X. grisescens. However, these impacts have also been softened by the species' biological characteristics, such as its relatively high capacity for movement. This study provides insights into how habitat changes might impact the long‐term survival of large solitary bees.

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Microbiome and floral associations of a wild bee using biodiversity survey collections

Nguyen,

Samad‐zada,

Chau

et al. 2024

Environmental Microbiology

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The health of bees can be assessed through their microbiome, which serves as a biomarker indicating the presence of both beneficial and harmful microorganisms within a bee community. This study presents the characterisation of the bacterial, fungal, and plant composition on the cuticle of adult bicoloured sweat bees (Agapostemon virescens). These bees were collected using various methods such as pan traps, blue vane traps and sweep netting across the northern extent of their habitat range. Non‐destructive methods were employed to extract DNA from the whole pinned specimens of these wild bees. Metabarcoding of the 16S rRNA, ITS and rbcL regions was then performed. The study found that the method of collection influenced the detection of certain microbial and plant taxa. Among the collection methods, sweep net samples showed the lowest fungal alpha diversity. However, minor differences in bacterial or fungal beta diversity suggest that no single method is significantly superior to others. Therefore, a combination of techniques can cater to a broader spectrum of microbial detection. The study also revealed regional variations in bacterial, fungal and plant diversity. The core microbiome of A. virescens comprises two bacteria, three fungi and a plant association, all of which are commonly detected in other wild bees. These core microbes remained consistent across different collection methods and locations. Further extensive studies of wild bee microbiomes across various species and landscapes will help uncover crucial relationships between pollinator health and their environment.

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The use of pinned specimens helps uncover patterns of genetic differentiation and signatures of selection in a wild pollinator

Cited by 3 publications

References 77 publications

Genetic divergence and ecological adaptation of an eastern North American spring ephemeral Sanguinaria canadensis

Genetic divergence and ecological adaptation of an eastern North American spring ephemeral Sanguinaria canadensis

Large carpenter bees show high dispersal in a tropical semi‐arid region susceptible to desertification

Microbiome and floral associations of a wild bee using biodiversity survey collections

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